Structural basis for AcrVA4 inhibition of specific CRISPR-Cas12a

Autor: Gavin J Knott, Brady F Cress, Jun-Jie Liu, Brittney W Thornton, Rachel J Lew, Basem Al-Shayeb, Daniel J Rosenberg, Michal Hammel, Benjamin A Adler, Marco J Lobba, Michael Xu, Adam P Arkin, Christof Fellmann, Jennifer A Doudna

Publikováno v: eLife, Vol 8 (2019)

CRISPR-Cas systems provide bacteria and archaea with programmable immunity against mobile genetic elements. Evolutionary pressure by CRISPR-Cas has driven bacteriophage to evolve small protein inhibitors, anti-CRISPRs (Acrs), that block Cas enzyme fu

Externí odkaz: https://doaj.org/article/164dc30ebd294804bf4e661f484ab735

Broad-spectrum enzymatic inhibition of CRISPR-Cas12a

Autor: Marco J. Lobba, Gavin J. Knott, Brittney W. Thornton, Jun-Jie Liu, Basem Al-Shayeb, Kyle E. Watters, Jennifer A. Doudna

Publikováno v: Nature structural & molecular biology, vol 26, iss 4
Knott, Gavin J; Thornton, Brittney W; Lobba, Marco J; Liu, Jun-Jie; Al-Shayeb, Basem; Watters, Kyle E; et al.(2019). Broad-spectrum enzymatic inhibition of CRISPR-Cas12a.. Nature structural & molecular biology, 26(4), 315-321. doi: 10.1038/s41594-019-0208-z. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/72n7n3pp
Nature structural & molecular biology

Cas12a is a bacterial RNA-guided nuclease used widely for genome editing and, more recently, as a molecular diagnostic. In bacteria, Cas12a enzymes can be inhibited by bacteriophage-derived proteins, anti-CRISPRs (Acrs), to thwart clustered regularly

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33b1124d75a71b561581bf7c713cb76a
https://doi.org/10.1038/s41594-019-0208-z

Structural basis for AcrVA4 inhibition of specific CRISPR-Cas12a

Autor: Michal Hammel, Marco J. Lobba, Gavin J. Knott, Basem Al-Shayeb, Rachel J. Lew, Daniel J. Rosenberg, Adam P. Arkin, Brittney W. Thornton, Benjamin A. Adler, Jennifer A. Doudna, Brady F. Cress, Christof Fellmann, Michael Xu, Jun-Jie Liu

Publikováno v: eLife, Vol 8 (2019)
eLife

CRISPR-Cas systems provide bacteria and archaea with programmable immunity against mobile genetic elements. Evolutionary pressure by CRISPR-Cas has driven bacteriophage to evolve small protein inhibitors, anti-CRISPRs (Acrs), that block Cas enzyme fu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3cd1afcd8cfb990c82936601658a4ab0
https://escholarship.org/uc/item/05w32397

DFT study of reductive functionalization in cis and trans cobalt–methyl–bipyridine complexes

Autor: Brittney W. Thornton, Dale R. Pahls, Franklin Jia, Shannon L. Teaw, Thomas R. Cundari, Justin Qian

Publikováno v: Computational and Theoretical Chemistry. 1073:102-105

Density functional theory (DFT) calculations were utilized to study the reductive functionalization (RF) of a bipyridine Co(III)–methyl complex, an integral step in metal-catalyzed hydrocarbon functionalization. In general, for the different X co-l

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc6952c8ff6db7b7cef6df906b42a51d
https://doi.org/10.1016/j.comptc.2015.09.021