Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Brittany N, Sprecher"'
Autor:
Brittany N. Sprecher, Jochen M. Buck, L. Loraine Ropella, Annette Ramsperger, Peter G. Kroth, Norico Yamada
Endosymbiosis is a widespread and ecologically significant phenomenon in the marine environment. How these endosymbiotic partners evolve into an organism with a new organelle is still mostly unknown and requires investigation into modern symbioses. D
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44400526b5e4d8ae40beb7e911875d1f
Publikováno v:
Microorganisms, Vol 8, Iss 1, p 126 (2020)
The lack of a robust gene transformation tool that allows proper expression of foreign genes and functional testing for the vast number of nuclear genes in dinoflagellates has greatly hampered our understanding of the fundamental biology in this ecol
Externí odkaz:
https://doaj.org/article/d4b3236af82a407493b5051401f6ab5d
Introducing CRISPR/Cas9 machinery into the cell as RNP complexes reduces the number of steps necessary to edit the genome, does not require codon optimization or a strong promoter, can result in higher editing efficiencies with fewer off target effec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2a7e1412542d53f3fe87b78171e7aa86
https://doi.org/10.17504/protocols.io.bei2jcge
https://doi.org/10.17504/protocols.io.bei2jcge
Autor:
Deepak Nanjappa, Iñaki Ruiz-Trillo, Christopher J. Howe, Chris Bowler, Mariusz Nowacki, Anastasios D. Tsaousis, Noelia Lander, Christopher L. Dupont, Shinichiro Maruyama, Fulei Luan, Andrew E. Allen, Anna M. G. Novák Vanclová, Pamela A. Silver, Nastasia J. Freyria, Peter von Dassow, Elisabeth Hehenberger, Konomi Fujimura-Kamada, Julius Lukeš, Roberto Docampo, Deborah L. Robertson, Thomas E. Clemente, Sebastián R. Najle, Kathryn J. Coyne, Cristina Aresté, Brittany N. Sprecher, Lu Wang, Eleanna Kazana, Verónica Freire-Benéitez, Vladimír Hampl, Cecilia Balestreri, Isabel C. Nimmo, Mariana Rius, Yoshihisa Hirakawa, Arnab Pain, Jorge Ibañez, Elin Einarsson, Lev Tsypin, Heriberto Cerutti, Adrian C. Barbrook, G. Jason Smith, Alexandra Z. Worden, Jian Guo, Jean Claude Lozano, Virginia P. Edgcomb, Huan Zhang, Andrea Highfield, Isaac Núñez, Fatma Gomaa, Jan Pyrih, Natalia Ewa Janowicz, Sara J. Bender, Ross F. Waller, Tobias von der Haar, François-Yves Bouget, Matus Valach, Amanda Hopes, Luke M. Noble, Paulo A. Garcia, Nicholas A.T. Irwin, Tamara Matute, Jernej Turnšek, Ian Hu, Sandra Pucciarelli, Fernán Federici, Valérie Vergé, R. Ellen R. Nisbet, Miguel Angel Chiurillo, Mark Moosburner, Monika Abedin Sigg, Aaron P. Turkewitz, Albane Ruaud, Angela Piersanti, Sebastian G. Gornik, Peter R. Girguis, Adam C. Jones, Lawrence A. Klobutcher, Claudio H. Slamovits, Elena Casacuberta, Imen Lassadi, Elizabeth C. Cooney, Kodai Fukuda, Nicole King, Manuel Ares, Jonathan Z. Kaye, Lisa Sudek, Patrick Beardslee, Cristina Miceli, Xiaoxue Wen, Estienne C. Swart, Yuu Ishii, Ambar Kachale, Pia A. Elustondo, Esteban R. Haro-Contreras, Patrick J. Keeling, José A. Fernández Robledo, Glen L. Wheeler, Colin Brownlee, Rowena Stern, Joshua S. Rest, Susana A. Breglia, Senjie Lin, Duncan B. Coles, Jackie L. Collier, Drahomíra Faktorová, David S. Booth, April Woods, Binnypreet Kaur, Thomas Mock, Gertraud Burger, Jun Minagawa, Yutaka Hanawa, Zhu-Hong Li, Rachele Cesaroni
Publikováno v:
Nature Methods
Nature Methods, Nature Publishing Group, 2020, 17 (5), pp.481-494. ⟨10.1038/s41592-020-0796-x⟩
Nature Methods, Nature Publishing Group, 2020, ⟨10.1038/s41592-020-0796-x⟩
Digital.CSIC. Repositorio Institucional del CSIC
instname
Nature Methods, Nature Publishing Group, 2020, 17 (5), pp.481-494. ⟨10.1038/s41592-020-0796-x⟩
Nature Methods, Nature Publishing Group, 2020, ⟨10.1038/s41592-020-0796-x⟩
Digital.CSIC. Repositorio Institucional del CSIC
instname
Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2626a2a63747a5e98954e7333b8e730
http://hdl.handle.net/11581/428891
http://hdl.handle.net/11581/428891
Publikováno v:
PLoS ONE, Vol 7, Iss 5, p e36744 (2012)
BACKGROUND: The microaerophilic bacterium Azorhizobium caulinodans, when fixing N(2) both in pure cultures held at 20 µM dissolved O(2) tension and as endosymbiont of Sesbania rostrata legume nodules, employs a novel, respiratory-membrane endo-hydro
Externí odkaz:
https://doaj.org/article/d9fe903625dd414e861b93378dcc3c69
Publikováno v:
Harmful Algae. 110:102136
First found in Korean coastal water, the dinoflagellate Gyrodinium jinhaense is a recently established species with unclear global distribution and unexplored genomic characteristics. From a laboratory fish mortality event off Long Island Sound, USA,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f4931b516d21285df5f7ee54788a999
https://doi.org/10.1016/j.hal.2021.102136
https://doi.org/10.1016/j.hal.2021.102136
This protocol is intended to work for many dinoflagellates but focusses on the method used to successfully transform Karlodinium veneficum.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5be2bb54bd03f3bbf5d3fcecb2bb4f9c
https://doi.org/10.17504/protocols.io.7n8hmhw
https://doi.org/10.17504/protocols.io.7n8hmhw
The lack of a robust gene transformation tool that allows functional testing of the vast number of nuclear genes in dinoflagellates has greatly hampered our understanding of fundamental biology in this ecologically important and evolutionarily unique
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f9923030c53ae6e764181cf4866a748
https://doi.org/10.1101/602821
https://doi.org/10.1101/602821
Autor:
Claudio H. Slamovits, Gertraud Burger, Patrick J. Keeling, Jian Guo, Fatma Gomaa, Jan Pyrih, Jun Minagawa, Christopher L. Dupont, Verónica Freire-Benéitez, Nastasia J. Freyria, Rachele Cesaroni, Elizabeth C. Cooney, Eleanna Kazana, Aaron P. Turkewitz, Peter R. Girguis, Konomi Fujimura-Kamada, Chris Bowler, Anastasios D. Tsaousis, Pamela A. Silver, Lawrence A. Klobutcher, Tamara Matute, Shinichiro Maruyama, Nicole King, Virginia P. Edgcomb, Roberto Docampo, Isaac Núñez, Deborah L. Robertson, Xiaoxue Wen, Zhu-Hong Li, Huan Zhang, Jorge Ibañez, Kathryn J. Coyne, Jonathan Z. Kaye, Christopher J. Howe, Andrew E. Allen, Ross F. Waller, Brittany N. Sprecher, R. Ellen R. Nisbet, Mark Moosburner, Alexandra Z. Worden, Thomas Mock, Angela Piersanti, Sebastian G. Gornik, Paulo A. Garcia, Amanda Hopes, Isabel C. Nimmo, Mariana Rius, Estienne C. Swart, Nicholas A.T. Irwin, Elisabeth Hehenberger, Valérie Vergé, Andrea Highfield, Sandra Pucciarelli, François-Yves Bouget, Yutaka Hanawa, Matus Valach, April Woods, Elin Einarsson, Anna M. G. Novák Vanclová, Lu Wang, Fernán Federici, Monika Abedin Sigg, Adrian C. Barbrook, Yoshihisa Hirakawa, G. Jason Smith, Adam C. Jones, Deepak Nanjappa, Vladimír Hampl, Binnypreet Kaur, Thomas E. Clemente, Jernej Turnšek, Jean Claude Lozano, Sara J. Bender, Ian Hu, Sebastián R. Najle, Imen Lassadi, Glen L. Wheeler, Cecilia Balestreri, Fulei Luan, Lev Tsypin, Heriberto Cerutti, Miguel Angel Chiurillo, Colin Brownlee, Rowena Stern, Julius Lukeš, Manuel Ares, Drahomíra Faktorová, Kodai Fukuda, David S. Booth, Peter von Dassow, Elena Casacuberta, Patrick Beardslee, Cristina Miceli, Cristina Aresté, Natalia Ewa Janowicz, Joshua S. Rest, Susana A. Breglia, Senjie Lin, Luke M. Noble, Albane Ruaud, Duncan B. Coles, Pia A. Elustondo, Jackie L. Collier, Lisa Sudek, Yuu Ishii, José A. Fernández Robledo, Noelia Lander, Tobias von der Haar, Ambar Kachale, Esteban R. Haro-Contreras, Arnab Pain, Iñaki Ruiz-Trillo, Mariusz Nowacki
Publikováno v:
Nature Methods
Author(s): Faktorova, Drahomira; Nisbet, R Ellen R; Fernandez Robledo, Jose A; Casacuberta, Elena; Sudek, Lisa; Allen, Andrew E; Ares, Manuel; Areste, Cristina; Balestreri, Cecilia; Barbrook, Adrian C; Beardslee, Patrick; Bender, Sara; Booth, David S
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c47aeb9d6357c08f30d7086fe183e393
http://europepmc.org/articles/PMC7200595
http://europepmc.org/articles/PMC7200595