Zobrazeno 1 - 10
of 229
pro vyhledávání: '"BrisSynBio"'
Autor:
Francesco Aulicino, Martin Pelosse, Christine Toelzer, Julien Capin, Erwin Ilegems, Parisa Meysami, Ruth Rollarson, Per-Olof Berggren, Mark Simon Dillingham, Christiane Schaffitzel, Moin A Saleem, Gavin I Welsh, Imre Berger
Publikováno v:
Aulicino, F, Pelosse, M, Toelzer, C, Capin, J M, Meysami, P, Dillingham, M S, Berger-Schaffitzel, C H, Saleem, M, Welsh, G I, Berger, I & et, A 2022, ' Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus ', Nucleic Acids Research, vol. 50, no. 13, pp. 7783–7799 . https://doi.org/10.1093/nar/gkac587
CRISPR-based precise gene-editing requires simultaneous delivery of multiple components into living cells, rapidly exceeding the cargo capacity of traditional viral vector systems. This challenge represents a major roadblock to genome engineering app
Autor:
William M. Dawson, Kathryn L. Shelley, Jordan M. Fletcher, D. Arne Scott, Lucia Lombardi, Guto G. Rhys, Tania J. LaGambina, Ulrike Obst, Antony J. Burton, Jessica A. Cross, George Davies, Freddie J. O. Martin, Francis J. Wiseman, R. Leo Brady, David Tew, Christopher W. Wood, Derek N. Woolfson
Publikováno v:
Dawson, W M, Shelley, K L, Fletcher, J M, Scott, D A, Lombardi, L, Rhys, G G, LaGambina, T J, Obst, U, Burton, A J, Cross, J A, Davies, G, Martin, F J O, Wiseman, F J, Brady, R L, Tew, D, Wood, C W & Woolfson, D N 2023, ' Differential sensing with arrays of de novo designed peptide assemblies ', Nature Communications, vol. 14, no. 1, 383 . https://doi.org/10.1038/s41467-023-36024-y
Differential sensing attempts to mimic the mammalian senses of smell and taste to identify analytes and complex mixtures. In place of hundreds of complex, membrane-bound G-protein coupled receptors, differential sensors employ arrays of small molecul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::540732c89dba6731409adb2cae642176
https://research-information.bris.ac.uk/en/publications/971c6c98-608d-4a82-a57c-d8e354786145
https://research-information.bris.ac.uk/en/publications/971c6c98-608d-4a82-a57c-d8e354786145
Publikováno v:
Bowen, J I, Wang, L, Crump, M P & Willis, C L 2022, ' Synthetic and Biosynthetic Methods for Selective Cyclisations of 4,5-Epoxy Alcohols to Tetrahydropyrans ', Organic and Biomolecular Chemistry, vol. 20, no. 6, 20, pp. 1150-1175 . https://doi.org/10.1039/D1OB01905H
Tetrahydropyrans (THPs) are common structural motifs found in natural products and synthetic therapeutic molecules. In Nature these 6-membered oxygen heterocycles are often assembled via intramolecular reactions involving either oxy-Michael additions
Autor:
Alice Halliday, Anna E. Long, Holly E. Baum, Amy C. Thomas, Kathryn L. Shelley, Elizabeth Oliver, Kapil Gupta, Ore Francis, Maia Kavanagh Williamson, Natalie Di Bartolo, Matthew J. Randell, Yassin Ben-Khoud, Ilana Kelland, Georgina Mortimer, Olivia Ball, Charlie Plumptre, Kyla Chandler, Ulrike Obst, Massimiliano Secchi, Lorenzo Piemonti, Vito Lampasona, Joyce Smith, Michaela Gregorova, Lea Knezevic, Jane Metz, Rachael Barr, Begonia Morales-Aza, Jennifer Oliver, Lucy Collingwood, Benjamin Hitchings, Susan Ring, Linda Wooldridge, Laura Rivino, Nicholas Timpson, Jorgen McKernon, Peter Muir, Fergus Hamilton, David Arnold, Derek N. Woolfson, Anu Goenka, Andrew D. Davidson, Ashley M. Toye, Imre Berger, Mick Bailey, Kathleen M. Gillespie, Alistair J. K. Williams, Adam Finn
Publikováno v:
Halliday, A, Long, A E, Baum, H E, Thomas, A, Shelley, K L, Oliver, E H, Gupta, K, Francis, O, Kavanagh Williamson, M, Di Bartolo, N D, Randell, M J, Ben Khoud, Y, Kelland, I, Mortimer, G L M, Ball, O E, Plumptre, C, Chandler, M A, Obst, U, Secchi, M, Piemonti, L, Lampasona, V, Smith, J, Gregorova, M, Knezevic, L, Metz, J, Barr, R S, Morales-Aza, M B, Oliver, J L, Collingwood, L, Hitchings, B E, Ring, S M, Wooldridge, L, Rivino, L, Timpson, N J, McKernon, J, Muir, P, Hamilton, F W, Arnold, D T, Woolfson, D N, Goenka, A, Davidson, A D, Toye, A M, Berger, I, Bailey, M, Gillespie, K M, Williams, A J K & Finn, A 2022, ' Development and evaluation of low-volume tests to detect and characterize antibodies to SARS-CoV-2 ', Frontiers in Immunology, vol. 13, 968317 . https://doi.org/10.3389/fimmu.2022.968317
Low-volume antibody assays can be used to track SARS-CoV-2 infection rates in settings where active testing for virus is limited and remote sampling is optimal. We developed 12 ELISAs detecting total or antibody isotypes to SARS-CoV-2 nucleocapsid, s
Publikováno v:
Ciurkot, K W, Gorochowski, T E, Roubos, J A & Verwaal, R 2021, ' Efficient multiplexed gene regulation in Saccharomyces cerevisiae using dCas12a ', Nucleic Acids Research, vol. 49, no. 13, gkab529, pp. 7775-7790 . https://doi.org/10.1093/nar/gkab529
Nucleic Acids Research
Nucleic Acids Research
CRISPR Cas12a is an RNA-programmable endonuclease particularly suitable for gene regulation. This is due to its preference for T-rich PAMs that allows it to more easily target AT-rich promoter sequences, and built-in RNase activity which can process
Publikováno v:
Stennett, H L, Back, C & Race, P R 2022, ' Derivation of A Precise and Consistent Timeline for Antibiotic Development ', Antibiotics, vol. 11, no. 9, 1237, pp. 1-12 . https://doi.org/10.3390/antibiotics11091237
Antibiotic resistance is a global health crisis. New classes of antibiotics that can treat drug-resistant infections are urgently needed. To communicate this message, researchers have used antibiotic development timelines, but these are often contrad
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2572f7f8d3c2e422a65318c104dc751c
https://research-information.bris.ac.uk/en/publications/9308e376-cb8d-48ed-90ad-cb496afd051e
https://research-information.bris.ac.uk/en/publications/9308e376-cb8d-48ed-90ad-cb496afd051e
Autor:
Ashley J. Winter, Matthew T. Rowe, Angus N. M. Weir, Nahida Akter, Sbusisiwe Z. Mbatha, Paul D. Walker, Christopher Williams, Zhongshu Song, Paul R. Race, Christine L. Willis, Matthew P. Crump
Publikováno v:
Winter, A J, Rowe, M T, Weir, A N M, Akter, N, Mbatha, S Z, Walker, P D, Williams, C, Song, Z, Race, P R, Willis, C L & Crump, M P 2022, ' Programmed Iteration Controls the Assembly of the Nonanoic Acid Side Chain of the Antibiotic Mupirocin ', Angewandte Chemie-International Edition, vol. 61, no. 50, e202212393 . https://doi.org/10.1002/anie.202212393
Mupirocin is a clinically important antibiotic produced by Pseudomonas fluorescens NCIMB 10586 that is assembled by a complex trans-AT polyketide synthase. The polyketide fragment, monic acid, is esterified by a 9-hydroxynonanoic acid (9HN) side chai
Autor:
Elise A. Naudin, Katherine I. Albanese, Abigail J. Smith, Bram Mylemans, Emily G. Baker, Orion D. Weiner, David M. Andrews, Natalie Tigue, Nigel J. Savery, Derek N. Woolfson
Publikováno v:
Naudin, E A, Albanese, K I, Smith, A J, Mylemans, B, Baker, E G, Savery, N J, Woolfson, D N & et, A 2022, ' From peptides to proteins : coiled-coil tetramers to single-chain 4-helix bundles ', Chemical Science, vol. 13, no. 38, pp. 11330-11340 . https://doi.org/10.1039/D2SC04479J
The design of completely synthetic proteins from first principles—de novo protein design—is challenging. This is because, despite recent advances in computational protein-structure prediction and design, we do not understand fully the sequence-to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49de030d354e6b82f0d5fa2d6401d9fd
https://doi.org/10.1101/2022.08.04.502660
https://doi.org/10.1101/2022.08.04.502660
Publikováno v:
Morris, N M, Blee, J & Hauert, S 2022, ' Developing a Computational Pharmacokinetic Model of Systemic Snakebite Envenomation and Antivenom Treatment ', Toxicon, vol. 215, pp. 77-90 . https://doi.org/10.1016/j.toxicon.2022.06.006
Snakebite envenomation is responsible for over 100,000 deaths and 400,000 cases of disability annually, most of which are preventable through access to safe and effective antivenoms. Snake venom toxins span a wide molecular weight range, influencing
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b39a82e527bafea06ccc5ba17d8bb5f
https://hdl.handle.net/1983/c512e436-c04d-4e59-9297-06bbc91410c4
https://hdl.handle.net/1983/c512e436-c04d-4e59-9297-06bbc91410c4
Publikováno v:
Elseginy, S A, Oliveira, S, Shoemark, D K & Sessions, R B 2022, ' Identification and validation of novel microtubule suppressors with an imidazopyridine scaffold through structure-based virtual screening and docking ', RSC medicinal chemistry, vol. 13, no. 8, pp. 929-943 . https://doi.org/10.1039/D1MD00392E
Targeting the colchicine binding site of α/β tubulin microtubules can lead to suppression of microtubule dynamics, cell cycle arrest and apoptosis. Therefore, the development of microtubule (MT) inhibitors is considered a promising route to antican
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::548d2e2b29d45180b670ba1a6be51b5c
https://research-information.bris.ac.uk/ws/files/333784347/d1md00392e.pdf
https://research-information.bris.ac.uk/ws/files/333784347/d1md00392e.pdf