Validation of Golden Gate assemblies using highly multiplexed Nanopore amplicon sequencing

Autor: Rojas, Adan A. Ramirez, Brinkmann, Cedric K., Schindler, Daniel

Golden Gate cloning has revolutionized synthetic biology. Its concept of modular, highly characterized libraries of parts that can be combined into higher order assemblies allows engineering principles to be applied to biological systems. The basic p

Externí odkaz: http://arxiv.org/abs/2401.14191

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DuBA.flowA Low-Cost, Long-Read Amplicon Sequencing Workflow for the Validation of Synthetic DNA Constructs.

Autor: Ramírez Rojas, Adán A., Brinkmann, Cedric K., Köbel, Tania S., Schindler, Daniel

Publikováno v: ACS Synthetic Biology; 2/16/2024, Vol. 13 Issue 2, p457-465, 9p

Validation of Golden Gate Assemblies Using Highly Multiplexed Nanopore Amplicon Sequencing.

Autor: Ramírez Rojas AA; Max Planck Institute for Terrestrial Microbiology, Marburg, Germany., Brinkmann CK; Max Planck Institute for Terrestrial Microbiology, Marburg, Germany., Schindler D; Max Planck Institute for Terrestrial Microbiology, Marburg, Germany. daniel.schindler@mpi-marburg.mpg.de.; Center for Synthetic Microbiology (SYNMIKRO), Philipps-University Marburg, Marburg, Germany. daniel.schindler@mpi-marburg.mpg.de.

Publikováno v: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2850, pp. 171-196.