Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Brigitte von Wilcken-Bergmann"'
Autor:
Bernard Révet, Benno Müller-Hill, Andrew Barker, Brigitte von Wilcken-Bergmann, Heike Bessert
Publikováno v:
Current Biology. 9:151-154
Transcription factors that are bound specifically to DNA often interact with each other over thousands of base pairs [1] [2]. Large DNA loops resulting from such interactions have been observed in Escherichia coli with the transcription factors deoR
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed4dbc9a717ac5940b1f4a3593d8ac9b
https://doi.org/10.1016/s0960-9822(99)80069-4
https://doi.org/10.1016/s0960-9822(99)80069-4
Autor:
Benno Müller-Hill, Brigitte von Wilcken-Bergmann, Manfred Schmidt, Harald Sobek, Daniel Schlieper
Publikováno v:
Analytical Biochemistry. 257:203-209
We have constructed a cloning vector with a tight positive selection for recombinant clones inEscherichia coli.The positive selection pressure results from a lethal mutation within theE. coligene coding for the catabolite gene activator protein CAP,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a6ae96ad4fd7e02f9397289e07154d7
https://doi.org/10.1006/abio.1997.2558
https://doi.org/10.1006/abio.1997.2558
Publikováno v:
Molecular Microbiology. 20:375-384
Summary The α-centred trp operator binds one dimer of the Trp repressor, whereas the β-centred trp operator binds two dimers of the Trp repressor (Carey et al., 1991; Haran et al., 1992). The Trp repressor with a Tyr-Gly-7 substitution binds almost
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3c148285d5e6007fb5fd50f22ddff37e
https://doi.org/10.1111/j.1365-2958.1996.tb02624.x
https://doi.org/10.1111/j.1365-2958.1996.tb02624.x
Autor:
Manfred Suckow, Brigitte von Wilcken-Bergmann, Benno Müller-Hill, Brigitte Kisters-Woike, Klaus Schwamborn
Publikováno v:
Nucleic Acids Research. 22:4395-4404
Two residues are invariant in all bZip basic regions: asparagine -18 and arginine -10 (we define the first leucine of the leucine zipper of GCN4 as +1). X-ray structures of two specific GCN4-DNA complexes (Ellenberger et al., Cell, 71, 1223-1237, 199
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a4f7745a01c342c08991ab8ed2186b0
https://doi.org/10.1093/nar/22.21.4395
https://doi.org/10.1093/nar/22.21.4395
Publikováno v:
Nucleic Acids Research. 21:2081-2086
The X-ray structure of a GCN4 DNA complex (1) shows, that specific DNA binding of the GCN4 basic region is mediated by a complicated network of base pair and DNA backbone contacts. According to the X-ray structure, alanine -14 of the basic region of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81466588d0ddc1c47e62a326b2f268e8
https://doi.org/10.1093/nar/21.9.2081
https://doi.org/10.1093/nar/21.9.2081
Autor:
Norbert Lehming, Brigitte von Wilcken-Bergmann, Benno Müller-Hill, Brigitte Kisters-Woike, J. Sartorius
Publikováno v:
Journal of Molecular Biology. 218:313-321
We constructed expression libraries for Lac repressor mutants with amino acid exchanges in positions 1, 2, 5 and 9 of the recognition helix. We then analysed the interactions of residues 5 and 9 with operator variants bearing single or multiple symme
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97457d16617e49c785fe06f23cb0913c
https://doi.org/10.1016/0022-2836(91)90714-h
https://doi.org/10.1016/0022-2836(91)90714-h
Linker mutagenesis in the lacZ gene of Escherichia coli yields variants of active beta-galactosidase
Publikováno v:
European Journal of Biochemistry. 195:191-194
Synthetic octameric oligonucleotides that code for a unique restriction site were cloned into a randomly linearized plasmid that carries the lacZ gene. The insertions were mapped by digestion with appropriate restriction endonucleases. 12 mutants wer
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::409777986cb30e0c8b3e5718125e03e2
https://doi.org/10.1111/j.1432-1033.1991.tb15694.x
https://doi.org/10.1111/j.1432-1033.1991.tb15694.x
Autor:
Barbara Koldin, Brigitte von Wilcken-Bergmann, Anke Seydel, Benno Müller-Hill, Manfred Suckow
The bZip proteins GCN4 and C/EBP differ in their DNA binding specificities: GCN4 binds well to the pseudopalindromic AP1 site 5'-A4T3G2A1C0T1C2'A3'T4'-3' and to the palindromic ATF/CREB sequence 5'-A4T3G2A1-C0*G0'T1'C2'A3'T4'-3'; C/EBP preferentially
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::345c154cc143033918ed2d2cfc3103fb
https://europepmc.org/articles/PMC307358/
https://europepmc.org/articles/PMC307358/
Autor:
Brigitte von Wilcken-Bergmann, Manfred Suckow, Anup Madan, Benno Müller-Hill, Brigitte Kisters-Woike
Publikováno v:
Nucleic acids research. 22(12)
The specificity of the GCN4/DNA complex is mediated by a complicated network of interactions between the basic regions of both GCN4 monomers and their target halfsites. According to X-ray analyses (1, 2) one particular thymine of the target sequence
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a3e1894426a2410b5b47cc75eb3e741
https://pubmed.ncbi.nlm.nih.gov/8036145
https://pubmed.ncbi.nlm.nih.gov/8036145
Autor:
Benno Müller-Hill, J. Sartorius, Norbert Lehming, Brigitte Kisters-Woike, Brigitte von Wilcken-Bergmann
Publikováno v:
European journal of biochemistry. 198(2)
Computer graphics were used to build a molecular model of the complex of Lac repressor and lac operator. The model is based (a) on the NMR data of the Kaptein group [Boelens, R., Lamerichs, R. M. J. N., Rullmann, J. A. C., van Boom, J. H. & Kaptein,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a42d4fbf45a585532107be3d6a6f15d
https://pubmed.ncbi.nlm.nih.gov/2040302
https://pubmed.ncbi.nlm.nih.gov/2040302