Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Brigitte Barg-Kues"'
Autor:
Khursheed Iqbal, Brigitte Barg-Kues, Sandra Broll, Jürgen Bode, Heiner Niemann, Wilfried A. Kues
Publikováno v:
BioTechniques, Vol 47, Iss 5, Pp 959-968 (2009)
Injection of linearized DNA constructs into the pronuclei of fertilized mammalian eggs is a standard method for producing transgenic embryos and animals. Here, we show that injection of covalently closed circular (ccc) plasmids into the cytoplasm of
Externí odkaz:
https://doaj.org/article/cc3b7210f9f6432eafd81cb404af0030
Autor:
Wiebke Garrels, Stephanie Holler, Ulrike Taylor, Doris Herrmann, Christina Struckmann, Sabine Klein, Brigitte Barg-Kues, Monika Nowak-Imialek, Christine Ehling, Detlef Rath, Zoltán Ivics, Heiner Niemann, Wilfried A Kues
Publikováno v:
PLoS ONE, Vol 6, Iss 11, p e27563 (2011)
Recently, we generated transposon-transgenic boars (Sus scrofa), which carry three monomeric copies of a fluorophore marker gene. Amazingly, a ubiquitous fluorophore expression in somatic, as well as in germ cells was found. Here, we characterized th
Externí odkaz:
https://doaj.org/article/4580cb0f34b24cce8022bca57e445be0
Autor:
Karin Kühnel, Janina Metje-Sprink, Johannes Groffmann, Ralf Ficner, Amanda M. Schalk, Brigitte Barg-Kues, Beyenech Binotti, Piotr Neumann
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
Scientific Reports
Scientific Reports
The Atg12-Atg5/Atg16L1 complex is recruited by WIPI2b to the site of autophagosome formation. Atg16L1 is an effector of the Golgi resident GTPase Rab33B. Here we identified a minimal stable complex of murine Rab33B(30–202) Q92L and Atg16L1(153–21
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b5162a0be3f76b15ba8026aabfabcb9
https://doi.org/10.1038/s41598-020-69637-0
https://doi.org/10.1038/s41598-020-69637-0
Autor:
Mikkel A. Rasmussen, Ivana Grabundzija, Zoltán Ivics, Poul Hyttel, Vanessa Jane Hall, Monika Nowak-Imialek, Brigitte Barg-Kues, Ulrich Martin, Heiner Niemann, Wilfried A. Kues, Sylvia Merkert, Antonia Grebe, Miriam Streeck, Thomas Buchholz, Doris Herrmann, Srividiameena Haridoss
Publikováno v:
Stem Cells and Development; Vol 22
Kues, W A, Herrmann, D, Barg-Kues, B, Haridoss, S, Nowak-Imialek, M, Buchholz, T, Streeck, M, Grebe, A, Grabundzija, I, Merkert, S, Martin, U, Hall, V J, Rasmussen, M A, Ivics, Z, Hyttel, P & Niemann, H 2013, ' Derivation and characterization of sleeping beauty transposon-mediated porcine induced pluripotent stem cells ', Stem Cells and Development, vol. 22, no. 1, pp. 124-135 . https://doi.org/10.1089/scd.2012.0382
Kues, W A, Herrmann, D, Barg-Kues, B, Haridoss, S, Nowak-Imialek, M, Buchholz, T, Streeck, M, Grebe, A, Grabundzija, I, Merkert, S, Martin, U, Hall, V J, Rasmussen, M A, Ivics, Z, Hyttel, P & Niemann, H 2013, ' Derivation and characterization of sleeping beauty transposon-mediated porcine induced pluripotent stem cells ', Stem Cells and Development, vol. 22, no. 1, pp. 124-135 . https://doi.org/10.1089/scd.2012.0382
The domestic pig is an important large animal model for preclinical testing of novel cell therapies. Recently, we produced pluripotency reporter pigs in which the Oct4 promoter drives expression of the enhanced green fluorescent protein (EGFP). Here,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2c4e35d1cb278ac03411afc6a5da64f
https://doi.org/10.1089/scd.2012.0382
https://doi.org/10.1089/scd.2012.0382
Autor:
Doris Herrmann, Wolf Ramackers, Andreas Tiede, Reinhard Schwinzer, Lars Friedrich, Andrea Lucas-Hahn, Anna-Lisa Queißer, Wiebke Baars, Joseph Wallace Carnwath, Brigitte Barg-Kues, Johannes Klose, Heiner Niemann, Michael Winkler, Erika Lemme, Petra Hassel, Björn Petersen
Publikováno v:
Xenotransplantation. 18:355-368
Background: The major immunological hurdle to successful porcine-to-human xenotransplantation is the acute vascular rejection (AVR), characterized by endothelial cell (EC) activation and perturbation of coagulation. Heme oxygenase-1 (HO-1) and its de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c70a6cb5f44c09deb31a79032c5d5894
https://doi.org/10.1111/j.1399-3089.2011.00674.x
https://doi.org/10.1111/j.1399-3089.2011.00674.x
Autor:
Sandra Broll, Jürgen Bode, Heiner Niemann, Brigitte Barg-Kues, Wilfried A. Kues, Khursheed Iqbal
Publikováno v:
BioTechniques. 47:959-968
Injection of linearized DNA constructs into the pronuclei of fertilized mammalian eggs is a standard method for producing transgenic embryos and animals. Here, we show that injection of covalently closed circular (ccc) plasmids into the cytoplasm of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5198b8efdfc8d75459a82cbbe8dcbdc2
https://doi.org/10.2144/000113270
https://doi.org/10.2144/000113270
Autor:
Zsuzsanna Izsvák, Stephanie Holler, Wilfried A. Kues, Heiner Niemann, Thirumala Rao Talluri, Björn Petersen, Ayan Mukherjee, Brigitte Barg-Kues, Peter Köhler, Zoltán Ivics, Wiebke Garrels, Andrea Lucas-Hahn, Mike Diederich, Nicole Cleve
Publikováno v:
Transgenic research. 25(1)
Recently, we established the Sleeping Beauty transposon system for germ line competent transgenesis in the pig. Here, we extend this approach to re-target a transposon-tagged locus for a site-specific gene knock-in, and generated a syngeneic cohort o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::394c79aaee68cecaed677b4543f80681
https://pubmed.ncbi.nlm.nih.gov/26515985
https://pubmed.ncbi.nlm.nih.gov/26515985
Autor:
Wilfried A. Kues, Reinhard Schwinzer, Dagmar Wirth, Els Verhoeyen, Erika Lemme, Doris Herrmann, Brigitte Barg-Kues, Hansjörg Hauser, Kurt Wonigeit, Heiner Niemann, Hansjorg Hauser
Publikováno v:
The FASEB Journal. 20:1200-1202
The applicability of tightly regulated transgenesis in domesticated animals is severely hampered by the present lack of knowledge of regulatory mechanisms and the long generation intervals. To capitalize on the tightly controlled expression of mammal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b3ae4c2b28a21c5a6200c76f5118a90
https://doi.org/10.1096/fj.05-5415fje
https://doi.org/10.1096/fj.05-5415fje
Autor:
Christian Weissgerber, Ulrich Baulain, Thomas Haaf, Wilfried A. Kues, Julia Heinzmann, Brigitte Barg-Kues, Mike Diederich, Richard Reinhard, Heiner Niemann, Tamara Hansmann, Doris Herrmann, Patrick Aldag
Publikováno v:
Reproduction (Cambridge, England). 144(3)
The developmental capacity of oocytes from prepubertal cattle is reduced compared with their adult counterparts, and epigenetic mechanisms are thought to be involved herein. Here, we analyzed DNA methylation in three developmentally important, nonimp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7bd3644bf2185d3b445082c2aa5fe6b2
https://pubmed.ncbi.nlm.nih.gov/22733804
https://pubmed.ncbi.nlm.nih.gov/22733804
Autor:
Björn, Petersen, Wolf, Ramackers, Andrea, Lucas-Hahn, Erika, Lemme, Petra, Hassel, Anna-Lisa, Queisser, Doris, Herrmann, Brigitte, Barg-Kues, Joseph W, Carnwath, Johannes, Klose, Andreas, Tiede, Lars, Friedrich, Wiebke, Baars, Reinhard, Schwinzer, Michael, Winkler, Heiner, Niemann
Publikováno v:
Xenotransplantation. 18(6)
The major immunological hurdle to successful porcine-to-human xenotransplantation is the acute vascular rejection (AVR), characterized by endothelial cell (EC) activation and perturbation of coagulation. Heme oxygenase-1 (HO-1) and its derivatives ha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::852af14c142ca7b5dd8709510f9e2eeb
https://pubmed.ncbi.nlm.nih.gov/22168142
https://pubmed.ncbi.nlm.nih.gov/22168142