Zobrazeno 1 - 10
of 44
pro vyhledávání: '"Brian Y Chow"'
Autor:
Xue eHan, Brian Y Chow, Huihui eZhou, Nathan C Klapoetke, Amy eChuong, Reza eRajimehr, Aimei eYang, Michael V Baratta, Jonathan eWinkle, Robert eDesimone, Edward S Boyden
Publikováno v:
Frontiers in Systems Neuroscience, Vol 5 (2011)
Technologies for silencing the electrical activity of genetically-targeted neurons in the brain are important for assessing the contribution of specific cell types and pathways towards behaviors and pathologies. Recently we found that archaerhodopsin
Externí odkaz:
https://doaj.org/article/cb928562f61f49cc819b7f7a5e1ce68b
Autor:
William Benman, Saachi Datta, David Gonzalez-Martinez, Gloria Lee, Juliette Hooper, Grace Qian, Gabrielle Leavitt, Lana Salloum, Gabrielle Ho, Sharvari Mhatre, Michael S. Magaraci, Michael Patterson, Sevile G. Mannickarottu, Saurabh Malani, Jose L. Avalos, Brian Y. Chow, Lukasz J. Bugaj
Publikováno v:
Communications Biology, Vol 6, Iss 1, Pp 1-14 (2023)
Abstract The ability to perform sophisticated, high-throughput optogenetic experiments has been greatly enhanced by recent open-source illumination devices that allow independent programming of light patterns in single wells of microwell plates. Howe
Externí odkaz:
https://doaj.org/article/3efa70a4a8c0485dbec94aa00969e823
Autor:
Erin E. Berlew, Keisuke Yamada, Ivan A. Kuznetsov, Eleanor A. Rand, Chandler C. Ochs, Zaynab Jaber, Kevin H. Gardner, Brian Y. Chow
Publikováno v:
ACS Synth Biol
We describe the efficient creation of single-component optogenetic tools for membrane recruitment-based signaling perturbation using BcLOV4 technology. The workflow requires two plasmids to create six different domain arrangements of the dynamic memb
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6775151a882ee31f0d97615218d51b9
https://doi.org/10.1021/acssynbio.1c00604
https://doi.org/10.1021/acssynbio.1c00604
Optogenetic clustering is a versatile method to control protein activity in living cells, tissues, and organisms. Here we show that the BcLOV4 photoreceptor both clusters and translocates to the plasma membrane in response to blue light, representing
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4c6968065bdf00bc9cb0f67b2d85441c
https://doi.org/10.1101/2022.12.12.520131
https://doi.org/10.1101/2022.12.12.520131
Autor:
Saachi Datta, William Benman, David Gonzalez-Martinez, Gloria Lee, Juliette Hooper, Grace Qian, Gabrielle Leavitt, Lana Salloum, Gabrielle Ho, Sharvari Mhatre, Michael S. Magaraci, Michael Patterson, Sevile G. Mannickarottu, Saurabh Malani, Jose L. Avalos, Brian Y. Chow, Lukasz J. Bugaj
The ability to perform sophisticated, high-throughput optogenetic experiments has been greatly enhanced by recent open-source illumination devices that allow independent programming of light patterns in single wells of microwell plates. However, ther
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::258f4b0b97e930efc6a21365e2e1651d
https://doi.org/10.1101/2022.07.13.499906
https://doi.org/10.1101/2022.07.13.499906
Publikováno v:
Cell reports methods. 2(7)
We describe a modular computational framework for analyzing cell-wide spatiotemporal signaling dynamics in single-cell microscopy experiments that accounts for the experiment-specific geometric and diffractive complexities that arise from heterogeneo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c976c9ca98697eac7230cfe12bdf9dcf
https://pubmed.ncbi.nlm.nih.gov/35880018
https://pubmed.ncbi.nlm.nih.gov/35880018
Publikováno v:
Curr Opin Struct Biol
Optical induction of intracellular signaling by membrane-associated and integral membrane proteins allows spatiotemporally precise control over second messenger signaling and cytoskeletal rearrangements that are important to cell migration, developme
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74bcfb49a7d1ca2cac4046bd3377ba6b
https://doi.org/10.1016/j.sbi.2019.01.017
https://doi.org/10.1016/j.sbi.2019.01.017
Autor:
Karol P. Szymula, Andrew Clark, Sevile G. Mannickarottu, Michael S. Magaraci, Brian Y. Chow, Michael Patterson
Publikováno v:
Biochemistry. 58:468-473
Microplate readers are foundational instruments in experimental biology and bioengineering that enable multiplexed spectrophotometric measurements. To enhance their accessibility, we here report the design, construction, validation, and benchmarking
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::88f5956f365cd9761054848f5724d78b
https://doi.org/10.1021/acs.biochem.8b00952
https://doi.org/10.1021/acs.biochem.8b00952
Autor:
Keisuke Yamada, Brian Y. Chow, Joel D. Boerckel, Ivan A. Kuznetsov, Erin E. Berlew, Lukasz J. Bugaj
We created optogenetic tools to control RhoA GTPase, a central regulator of actin organization and actomyosin contractility. RhoA GTPase, or its upstream activating GEF effectors, were fused to BcLOV4, a photoreceptor that can be dynamically recruite
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a9c13f4ef2ecab8f0e078207d53ae464
https://doi.org/10.1101/2021.02.01.429147
https://doi.org/10.1101/2021.02.01.429147
Optogenetic Rac1 engineered from membrane lipid-binding RGS-LOV for inducible lamellipodia formation
Publikováno v:
Photochem Photobiol Sci
We report the construction of a single-component optogenetic Rac1 (opto-Rac1) to control actin polymerization by dynamic membrane recruitment. Opto-Rac1 is a fusion of wildtype human Rac1 small GTPase to the C-terminal region of BcLOV4, a LOV (light-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a81db0d3ca82fdcc1afbf0a16b9b9f6
https://pubmed.ncbi.nlm.nih.gov/32048687
https://pubmed.ncbi.nlm.nih.gov/32048687