Zobrazeno 1 - 10
of 84
pro vyhledávání: '"Brian R. Rabin"'
Publikováno v:
Enzyme and Microbial Technology. 16:281-285
Nonradioactive immunoassays incorporating an element of amplification in their detection system require the use of components that are highly purified. Flavin adenine dinucleotide-3′-phosphate (FADP) is the primary substrate used in such an amplifi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::506791b2b292336a52de0ef7f672b798
https://doi.org/10.1016/0141-0229(94)90167-8
https://doi.org/10.1016/0141-0229(94)90167-8
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1161:311-316
A mathematical model describing the behaviour of a new class of prosthetogenic enzyme amplification assays is described. The predictions of the model are favourably compared with an enzyme amplification assay for alkaline phosphatase. The model is us
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1db499cbe4c22b2b101bc37159ccb7b4
https://doi.org/10.1016/0167-4838(93)90230-o
https://doi.org/10.1016/0167-4838(93)90230-o
Autor:
Henny J. Eggelte, Stuart Harbron, Brian R. Rabin, Christopher John Taylorson, Antonio Tarragona-Fiol, John M. Ward, E. Sanchez
Publikováno v:
"Protein Engineering, Design and Selection". 6:901-906
In addition to hydrolysing RNA, bovine pancreatic ribonuclease splits esters of pyrimidine nucleoside 3'-phosphates, including dinucleotides. For a series of 3':5'-linked dinucleotides of general structure CpN, where N is a 5' linked nucleoside, kcat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ce59945d4162f352884aa89a372a08d5
https://doi.org/10.1093/protein/6.8.901
https://doi.org/10.1093/protein/6.8.901
Publikováno v:
Gene. 118:239-245
The coding sequence for the bovine pancreatic ribonuclease (RNase) precursor has been cloned and produced in Escherichia coli using the polymerase chain reaction (PCR) technique. A PCR amplification has been carried out utilizing as template the reco
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f0db94817fe5e3cf53fb95bad696dda
https://doi.org/10.1016/0378-1119(92)90194-t
https://doi.org/10.1016/0378-1119(92)90194-t
Publikováno v:
Biotechnology Techniques. 6:55-60
Dissociation of FAD from D-aminoacid oxidase occurred most rapidly at pH 6.0 in the presence of 1 M KBr. Diafiltration of 0.6 g of enzyme under these conditions yielded apoenzyme containing 1.3% of residual holoenzyme activity, which was subsequently
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8aca8d7f5d86e92c89e953bef49bbf0b
https://doi.org/10.1007/bf02438689
https://doi.org/10.1007/bf02438689
Publikováno v:
Analytical biochemistry. 227(1)
An amplification assay for the measurement of alkaline phosphatase has been combined with a luminescent end point using the luminol-peroxidase system to produce the first enzyme-amplified chemiluminescent assay based on the principle of prosthetogene
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9d45d4b93a1472be6d0083b9c24835b
https://pubmed.ncbi.nlm.nih.gov/7668394
https://pubmed.ncbi.nlm.nih.gov/7668394
Publikováno v:
Analytical biochemistry. 206(1)
A simple to use, robust, quantitative, and extremelysensitive colorimetric assay for alkaline phosphatase (EC 3.1.3.1), designed to be used as a detection system in diagnostic assays employing antibodies or gene probes, is described. This technology
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dedabe8cd67523c482f5f602ff6b6aba
https://pubmed.ncbi.nlm.nih.gov/1360771
https://pubmed.ncbi.nlm.nih.gov/1360771
Publikováno v:
Analytical biochemistry. 198(1)
Alkaline phosphatase hydrolyzes riboflavin 4'-phosphate to produce riboflavin. This is converted to riboflavin 5'-phosphate, using riboflavin kinase, which reconstitutes apoglycolate oxidase to give hologlycolate oxidase. This enzyme catalyzes the ox
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c31c04ba42ea3d66c8a5231dc2a36559
https://pubmed.ncbi.nlm.nih.gov/1789431
https://pubmed.ncbi.nlm.nih.gov/1789431
Publikováno v:
FEBS Letters. 59:225-229
It has been suggested that constitutive membrane proteins are synthesised on rough endoplasmic reticulum with subsequent translocation, where appropriate, to smooth membranes, [l-4]. However, alternative models may be possible and indeed evidence has
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a09b9b4ba6ac4b47e64cd23dea9b2f70
https://doi.org/10.1016/0014-5793(75)80380-2
https://doi.org/10.1016/0014-5793(75)80380-2
Publikováno v:
Biochemical Journal. 194:691-697
1. Cytochrome P-450 has been detected in preparations of golgi apparatus from the livers of phenobarbital-induced rats. 2. Newly biosynthesized cytochrome P-450 is also present in preparations of golgi apparatus. By using three different techniques t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c4c4f955039215e832a7c2821e8f57d
https://doi.org/10.1042/bj1940691
https://doi.org/10.1042/bj1940691