High-Level Production of Recombinant Snowdrop Lectin in Sugarcane and Energy Cane

Autor: Carmen S. Padilla, Mona B. Damaj, Zhong-Nan Yang, Joe Molina, Brian R. Berquist, Earl L. White, Nora Solís-Gracia, Jorge Da Silva, Kranthi K. Mandadi

Publikováno v: Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)

Sugarcane and energy cane (Saccharum spp. hybrids) are ideal for plant-based production of recombinant proteins because their high resource-use efficiency, rapid growth and efficient photosynthesis enable extensive biomass production and protein accu

Externí odkaz: https://doaj.org/article/c614ea5403614df09b68c10a5b358285

Plant‐made E2 glycoprotein single‐dose vaccine protects pigs against classical swine fever

Autor: Sylvain Marcel, Yair Peres, Chungwon J. Chung, John G. Neilan, Jishu Shi, L. Garry Adams, Lihua Wang, Erica Martel, Sreenath R. Palle, Rachel Madera, Sara D. Lawhon, David A. Brake, Yulia Burakova, Brian R. Berquist, Richard Laughlin, Sterling Buist, Max V. Rasmussen

Publikováno v: Plant Biotechnology Journal

Classical Swine Fever Virus (CSFV) causes classical swine fever, a highly contagious hemorrhagic fever affecting both feral and domesticated pigs. Outbreaks of CSF in Europe, Asia, Africa and South America had significant adverse impacts on animal he

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28744b2f9acd8157fbfa8af2640073e0
http://europepmc.org/articles/PMC6335066

Implementation of Glycan Remodeling to Plant-Made Therapeutic Antibodies

Autor: Zhongjie Ren, Lindsay Bennett, Barry R. Holtz, Lai-Xi Wang, Sylvain Marcel, Ryan P Murray, John P. Giddens, Brian R. Berquist, Earl L White, Qiang Yang, Vally Kommineni

Publikováno v: International Journal of Molecular Sciences, Vol 19, Iss 2, p 421 (2018)
International Journal of Molecular Sciences
International Journal of Molecular Sciences; Volume 19; Issue 2; Pages: 421

N-glycosylation profoundly affects the biological stability and function of therapeutic proteins, which explains the recent interest in glycoengineering technologies as methods to develop biobetter therapeutics. In current manufacturing processes, N-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90a9562fe146606ea4b6b83741f3979b
http://www.mdpi.com/1422-0067/19/2/421

CSB interacts with SNM1A and promotes DNA interstrand crosslink processing

Autor: David M. Wilson, Sook Y. Lee, Opher Gileadi, Peter J. McHugh, Michael M. Seidman, Vilhelm A. Bohr, Brian R. Berquist, Teruaki Iyama

Publikováno v: Nucleic Acids Research

Cockayne syndrome (CS) is a premature aging disorder characterized by photosensitivity, impaired development and multisystem progressive degeneration, and consists of two strict complementation groups, A and B. Using a yeast two-hybrid approach, we i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85e6212c963f52468e295039335669dc
https://ora.ox.ac.uk/objects/uuid:f9ae871b-4b9a-4ff2-85de-9c8095b80a89

Functional capacity of XRCC1 protein variants identified in DNA repair-deficient Chinese hamster ovary cell lines and the human population

Autor: Vilhelm A. Bohr, Jinshui Fan, Brian R. Berquist, Eric J. Ackerman, Dharmendra Kumar Singh, Alan E. Tomkinson, Avanti Kulkarni, Daemyung Kim, Elizabeth Gillenwater, David M. Wilson

Publikováno v: Nucleic Acids Research
Nucleic acids research, vol 38, iss 15

XRCC1 operates as a scaffold protein in base excision repair, a pathway that copes with base and sugar damage in DNA. Studies using recombinant XRCC1 proteins revealed that: a C389Y substitution, responsible for the repair defects of the EM-C11 CHO c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01ff45a40cefbf159d35a6ba6f5e57d5
http://europepmc.org/articles/PMC2926592