Uracil DNA N-glycosylase promotes assembly of human centromere protein A.

Autor: Samantha G Zeitlin, Brian R Chapados, Norman M Baker, Caroline Tai, Geir Slupphaug, Jean Y J Wang

Publikováno v: PLoS ONE, Vol 6, Iss 3, p e17151 (2011)

Uracil is removed from DNA by the conserved enzyme uracil DNA N-glycosylase (UNG). Previously, we observed that inhibiting UNG in Xenopus egg extracts blocked assembly of CENP-A, a histone H3 variant. CENP-A is an essential protein in all species, si

Externí odkaz: https://doaj.org/article/daa6d77585eb4b38b51f181627e19706

Double-strand DNA breaks recruit the centromeric histone CENP-A

Autor: Brian R. Chapados, Samantha G. Zeitlin, Evi Soutoglou, Michael W. Berns, Norman M. Baker, Don W. Cleveland, Jean Y. J. Wang

Publikováno v: Zeitlin, SG; Baker, NM; Chapados, BR; Soutoglou, E; Wang, JYJ; Berns, MW; et al.(2009). Double-strand DNA breaks recruit the centromeric histone CENP-A. Proceedings of the National Academy of Sciences of the United States of America, 106(37), 15762-15767. doi: 10.1073/pnas.0908233106. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/3z3776mp

The histone H3 variant CENP-A is required for epigenetic specification of centromere identity through a loading mechanism independent of DNA sequence. Using multiphoton absorption and DNA cleavage at unique sites by I- Sce I endonuclease, we demonstr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3cd87a4709da1776293190d0949d7992
https://doi.org/10.1073/pnas.0908233106

The C-terminal domain of yeast PCNA is required for physical and functional interactions with Cdc9 DNA ligase

Autor: John A. Tainer, Brian R. Chapados, Sangeetha Vijayakumar, Kristina H. Schmidt, Alan E. Tomkinson, Richard D. Kolodner

Publikováno v: Nucleic Acids Research

There is compelling evidence that proliferating cell nuclear antigen (PCNA), a DNA sliding clamp, co-ordinates the processing and joining of Okazaki fragments during eukaryotic DNA replication. However, a detailed mechanistic understanding of functio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4a0ba0528dcc8fcaf12c8bd6a419186
https://doi.org/10.1093/nar/gkm006

Dual modes of RNA-silencing suppression by Flock House virus protein B2

Autor: Brian R. Chapados, June Hyung Lee, Erik W. Debler, James R. Williamson, Anette Schneemann, Jeffrey A. Chao

Publikováno v: Nature Structural & Molecular Biology. 12:952-957

As a counter-defense against antiviral RNA silencing during infection, the insect Flock House virus (FHV) expresses the silencing suppressor protein B2. Biochemical experiments show that B2 binds to double-stranded RNA (dsRNA) without regard to lengt

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f753f9051d75a2f2503990a59c5e2b67
https://doi.org/10.1038/nsmb1005

Archaeoglobus fulgidus RNase HII in DNA Replication: Enzymological Functions and Activity Regulation via Metal Cofactors

Autor: Qing Chai, Junzhuan Qiu, Binghui Shen, Brian R. Chapados

Publikováno v: Biochemical and Biophysical Research Communications. 286:1073-1081

RNA primer removal during DNA replication is dependent on ribonucleotide- and structure-specific RNase H and FEN-1 nuclease activities. A specific RNase H involved in this reaction has long been sought. RNase HII is the only open reading frame in Arc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b47f41b025c38ec5b7874b9e4975469
https://doi.org/10.1006/bbrc.2001.5523