Zobrazeno 1 - 10
of 190
pro vyhledávání: '"Brian R, Crane"'
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103171- (2024)
Summary: Here, we present a protocol to quantify interactions among difficult-to-express proteins from Drosophila cells using the select western blot-free tagged-protein interaction (SWFTI) assay. We describe steps for plasmid design, cell plating, p
Externí odkaz:
https://doaj.org/article/63f979aa131f4ea3a13b457ed2186e02
Autor:
Cristina C. DeOliveira, Brian R. Crane
Publikováno v:
Frontiers in Chemistry, Vol 12 (2024)
Cryptochromes (CRYs), which are signaling proteins related to DNA photolyases, play pivotal roles in sensory responses throughout biology, including growth and development, metabolic regulation, circadian rhythm entrainment and geomagnetic field sens
Externí odkaz:
https://doaj.org/article/8647306035d042eb9c39cd82b23afb9a
Autor:
Daniyal Tariq, Nicole Maurici, Bradley M Bartholomai, Siddarth Chandrasekaran, Jay C Dunlap, Alaji Bah, Brian R Crane
Publikováno v:
eLife, Vol 12 (2024)
Circadian clocks are composed of transcription-translation negative feedback loops that pace rhythms of gene expression to the diurnal cycle. In the filamentous fungus Neurospora crassa, the proteins Frequency (FRQ), the FRQ-interacting RNA helicase
Externí odkaz:
https://doaj.org/article/cad2483ee3074190926abe1e0ea9cbce
Autor:
Ching Wooen Sze, Kai Zhang, Michael J Lynch, Radha Iyer, Brian R Crane, Ira Schwartz, Chunhao Li
Publikováno v:
PLoS Pathogens, Vol 19, Iss 11, p e1011752 (2023)
As an enzootic pathogen, the Lyme disease bacterium Borrelia burgdorferi possesses multiple copies of chemotaxis proteins, including two chemotaxis histidine kinases (CHK), CheA1 and CheA2. Our previous study showed that CheA2 is a genuine CHK that i
Externí odkaz:
https://doaj.org/article/720fc91ad121415f886661ccd209dc69
Autor:
Sahar Foroutannejad, Lydia L. Good, Changfan Lin, Zachariah I. Carter, Mahlet G. Tadesse, Aaron L. Lucius, Brian R. Crane, Rodrigo A. Maillard
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)
Characterizing folding pathways of large proteins that bind complex cofactors is challenging. The authors use optical tweezers to study the 542-residue FAD-binding lightsensor protein dCRY, identifying several intermediates and cofactor binding steps
Externí odkaz:
https://doaj.org/article/30dd6d9302b84ee08c76dff6d29b567b
Autor:
Siddarth Chandrasekaran, Connor M. Schneps, Robert Dunleavy, Changfan Lin, Cristina C. DeOliveira, Abir Ganguly, Brian R. Crane
Publikováno v:
Communications Biology, Vol 4, Iss 1, Pp 1-12 (2021)
Chandrasekaran et al. engineered the Drosophila circadian clock protein Cryptochrome (dCRY) to form the neutral semiquinone, which serves as a dark-state proxy. They find that the C-terminal tail of dCRY remains docked when the flavin ring is reduced
Externí odkaz:
https://doaj.org/article/239433bfd7aa4c559db2c188b3ff245d
Autor:
Alise R. Muok, Davi R. Ortega, Kurni Kurniyati, Wen Yang, Zachary A. Maschmann, Adam Sidi Mabrouk, Chunhao Li, Brian R. Crane, Ariane Briegel
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
The main components of the prokaryotic chemotaxis system, chemoreceptors, organize into a hexagonal (P6 symmetry) extended array. Here authors use cryo-ET and report an alternative symmetry (P2) of the chemotaxis apparatus that emerges from a strict
Externí odkaz:
https://doaj.org/article/0e767d14fc304f158716af0f1c3d659f
Publikováno v:
Nature. 617:194-199
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4deb56b18ff44d236e8c8c7cce555c2a
https://doi.org/10.1038/s41586-023-06009-4
https://doi.org/10.1038/s41586-023-06009-4
Publikováno v:
Acta Crystallographica Section D Structural Biology. 78:975-985
Fixed-target serial crystallography allows the high-throughput collection of diffraction data from small crystals at room temperature. This methodology is particularly useful for difficult samples that have sensitivity to radiation damage or intolera
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd4a24e3eef254717434222cfe2528ff
https://doi.org/10.1107/s2059798322007008
https://doi.org/10.1107/s2059798322007008
Enzymatic Spin-Labeling of Protein N- and C-Termini for Electron Paramagnetic Resonance Spectroscopy
Publikováno v:
Bioconjugate Chemistry.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3467b5d6a52bcfd987878ec0c41c9959
https://doi.org/10.1021/acs.bioconjchem.3c00029
https://doi.org/10.1021/acs.bioconjchem.3c00029