Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Brian J. Koebmann"'
Publikováno v:
Microbial Physiology. 18:174-180
The glycolytic enzyme phosphoglycerate mutase (PGM), which catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate, was examined in Lactococcus lactis with respect to its function, kinetics and glycolytic flux control. A library of strai
Publikováno v:
Technical University of Denmark Orbit
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has previously been suggested to have almost absolute control over the glycolytic flux in Lactococcus lactis (B. Poolman, B. Bosman, J. Kiers, and W. N. Konings, J. Bacteriol. 169:5887-5890, 1987). Tho
Publikováno v:
Applied and Environmental Microbiology. 68:5249-5257
Lactococcus lactis MBP71 Δ thyA (thymidylate synthase) cannot synthesize dTTP de novo, and DNA replication is dependent on thymidine in the growth medium. In the nonreplicating state acidification by MBP71 was completely insensitive to bacteriophage
Autor:
Hans V. Westerhoff, Barbara M. Bakker, Jacky L. Snoep, Klaas Krab, Johanna G. Koster, Frank J. Bruggeman, Rob J.M. van Spanning, Peter Ruhdal Jensen, M.F. Otten, Henk W. van Verseveld, Brian J. Koebmann, Sarah Koefoed
Publikováno v:
Koefoed, S, Otten, M F, Koebmann, B J, Bruggeman, F J, Bakker, B M, Snoep, J L, Krab, K, van Spanning, R J M, van Verseveld, H W, Jensen, P R, Koster, J G & Westerhoff, H V 2002, ' A turbo engine with automatic transmission? How to marry chemicomotion to the subtleties and robustness of life. ', Biochimica et Biophysica Acta (BBA)-Bioenergetics, vol. 1555, pp. 75-82 . https://doi.org/10.1016/S0005-2728(02)00258-X
Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1555, 75-82. Elsevier
Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1555, 75-82. Elsevier
Most genomes are much more complex than required for the minimum chemistry of life. Evolution has selected sophistication more than life itself. Could this also apply to bioenergetics? We first examine mechanisms through which bioenergetics could del
Publikováno v:
Koebmann, B J, Westerhoff, H V, Snoep, J L, Nilsson, D & Jensen, P R 2002, ' The glycolytic flux in Escherichia coli is controlled by the demand for ATP. ', Journal of Bacteriology, vol. 184, pp. 3909-3916 . https://doi.org/10.1128/JB.184.14.3909-3916.2002
Journal of Bacteriology, 184, 3909-3916. American Society for Microbiology
Technical University of Denmark Orbit
Journal of Bacteriology, 184, 3909-3916. American Society for Microbiology
Technical University of Denmark Orbit
The nature of the control of glycolytic flux is one of the central, as-yet-uncharacterized issues in cellular metabolism. We developed a molecular genetic tool that specifically induces ATP hydrolysis in living cells without interfering with other as
Publikováno v:
Antonie van Leeuwenhoek. 82:237-248
The understanding of control of metabolic processes requires quantitative studies of the importance of the different enzymatic steps for the magnitude of metabolic fluxes and metabolite concentrations. An important element in such studies is the modu
Autor:
Dan Nilsson, Jacky L. Snoep, Martin Bo Uhre Pedersen, Brian J. Koebmann, Ole Michelsen, Peter Ruhdal Jensen, Christian Solem, Hans V. Westerhoff
Publikováno v:
Molecular Biology Reports. 29:41-45
Using molecular genetics we have introduced uncoupled ATPase activity in two different bacterial species, Escherichia coli and Lactococcus lactis, and determined the elasticities of the growth rate and glycolytic flux towards the intracellular [ATP]/
Publikováno v:
Applied Microbiology and Biotechnology
Applied Microbiology and Biotechnology, Springer Verlag, 2011, 90 (2), pp.615-23. ⟨10.1007/s00253-010-3018-0⟩
Applied Microbiology and Biotechnology, 2011, 90 (2), pp.615-23. ⟨10.1007/s00253-010-3018-0⟩
Applied Microbiology and Biotechnology, Springer Verlag, 2011, 90 (2), pp.615-23. ⟨10.1007/s00253-010-3018-0⟩
Applied Microbiology and Biotechnology, 2011, 90 (2), pp.615-23. ⟨10.1007/s00253-010-3018-0⟩
International audience; Streptomyces are bacteria of industrial interest whose genome contains more than 73% of bases GC. In order to define, in these GC-rich bacteria, specific sequence features of strong promoters, a library of synthetic promoters
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e48954911387897a4b65de2e2c62d781
https://hal.archives-ouvertes.fr/hal-00591940
https://hal.archives-ouvertes.fr/hal-00591940
Publikováno v:
BioTechniques. 45(3)
System-oriented applications of genetic engineering, such as metabolic engineering, often require the serial optimization of enzymatic reaction steps, which can be achieved by transcriptional fine-tuning. However, approaches to changing gene expressi
Publikováno v:
IET systems biology. 2(2)
Triosephosphate isomerase (TPI), which catalyses the conversion of dihydroxyacetone phosphate (DHAP) to glyceraldehyde-3-phosphate (G3P), was studied for its control on glycolysis and mixed acid production in L. lactis subspecies lactis IL1403 and L.