An automated workflow to screen alkene reductases using high-throughput thin layer chromatography

Autor: Taek Soon Lee, Corey W. Meadows, Brett Garabedian, Raya Abourjeily, Joel M. Guenther, Tristan de Rond, Florence Mingardon

Publikováno v: Biotechnology for biofuels, vol 13, iss 1
Biotechnology for Biofuels, Vol 13, Iss 1, Pp 1-14 (2020)
Biotechnology for Biofuels

Background Synthetic biology efforts often require high-throughput screening tools for enzyme engineering campaigns. While innovations in chromatographic and mass spectrometry-based techniques provide relevant structural information associated with e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c9bde217d2d315890136b6ef3635948
https://doi.org/10.21203/rs.3.rs-52267/v3

Chemical Optimization of Whole-Cell Transfer Hydrogenation Using Carbonic Anhydrase as Host Protein

Autor: Thomas R. Ward, Yoann Cotelle, Johannes G. Rebelein, Brett Garabedian

Publikováno v: ACS Catalysis
ACS Catalysis, American Chemical Society, 2019, 9 (5), pp.4173-4178. ⟨10.1021/acscatal.9b01006⟩

[Image: see text] Artificial metalloenzymes combine a synthetic metallocofactor with a protein scaffold and can catalyze abiotic reactions in vivo. Herein, we report on our efforts to valorize human carbonic anhydrase II as a scaffold for whole-cell

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8daf38fce098fe5037b21644b121183
https://pubmed.ncbi.nlm.nih.gov/31080690

Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli

Autor: Brett Garabedian, Jay D. Keasling, Edward E. K. Baidoo, Taek Soon Lee, Christopher J. Petzold, Paul D. Adams, Tianwei Tan, Chun-Li Liu, Shuai Wang, Yan Chen, Tian Tian, Jorge Alonso-Gutierrez, Veronica T. Benites

Publikováno v: Biotechnology for Biofuels
Biotechnology for biofuels, vol 11, iss 1
Liu, CL; Tian, T; Alonso-Gutierrez, J; Garabedian, B; Wang, S; Baidoo, EEK; et al.(2018). Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli. Biotechnology for Biofuels, 11(1), 285. doi: 10.1186/s13068-018-1272-z. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/03s093vn
Biotechnology for Biofuels, Vol 11, Iss 1, Pp 1-15 (2018)
Liu, C-L, Tian, T, Alonso-Gutierrez, J, Garabedian, B, Wang, S, Baidoo, E E K, Benites, V, Chen, Y, Petzold, C J, Adams, P D, Keasling, J D, Tan, T & Lee, T S 2018, ' Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli ', Biotechnology for Biofuels, vol. 11, 285 . https://doi.org/10.1186/s13068-018-1272-z

Background Aviation fuels are an important target of biofuels research due to their high market demand and competitive price. Isoprenoids have been demonstrated as good feedstocks for advanced renewable jet fuels with high energy density, high heat o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4bb542b9c16cde0b7c05dce0534ee961
http://europepmc.org/articles/PMC6195743

Correction: E. coli surface display of streptavidin for directed evolution of an allylic deallylase

Autor: Tillmann Heinisch, Vitor B. Pinheiro, Markus Jeschek, Sven Panke, Thomas R. Ward, Eszter Csibra, Brett Garabedian, Jaicy Vallapurackal, Fabian Schwizer, Philippe Marlière

Publikováno v: Chemical Science

Correction for ‘E. coli surface display of streptavidin for directed evolution of an allylic deallylase’ by Tillmann Heinisch et al., Chem. Sci., 2018, 9, 5383–5388.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::430b173ecefbd5257597bd417a500a9a
https://doi.org/10.1039/c9sc90139f