Výsledky vyhledávání - "Bret R. Miller"

Engineered CRISPR prime editors with compact, untethered reverse transcriptases

Autor: Julian Grünewald, Bret R. Miller, Regan N. Szalay, Peter K. Cabeceiras, Christopher J. Woodilla, Eliza Jane B. Holtz, Karl Petri, J. Keith Joung

Publikováno v: Nature Biotechnology. 41:337-343

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::65fbe46e882add82bb41098d55a07286
https://doi.org/10.1038/s41587-022-01473-1

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CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells

Autor: J. Keith Joung, Ronghao Zhou, Sowmya Iyer, Bret R. Miller, Lukas M. Langner, Ibrahim C. Kurt, Julian Grünewald, Sara P. Garcia

Publikováno v: Nature biotechnology

CRISPR-guided DNA cytosine and adenine base editors (CBEs and ABEs) are widely used for many applications1–4 but primarily create DNA base transitions (i.e., pyrimidine-to-pyrimidine, or purine-to-purine). Here we describe the engineering of two ba

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c55363855f17e643e094eef4db9a52e3
https://doi.org/10.1038/s41587-020-0609-x

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A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing

Autor: Jonathan Y. Hsu, Martin J. Aryee, Ronghao Zhou, J. Keith Joung, Lukas M. Langner, Sara P. Garcia, Bret R. Miller, Julian Grünewald, Sowmya Iyer, Caleb A. Lareau

Publikováno v: Nature biotechnology

Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR-Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that ca

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4372198978a9c533af57b06ded868f53
https://doi.org/10.1038/s41587-020-0535-y

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Engineered CRISPR prime editors with compact, untethered reverse transcriptases

Autor: Julian, Grünewald, Bret R, Miller, Regan N, Szalay, Peter K, Cabeceiras, Christopher J, Woodilla, Eliza Jane B, Holtz, Karl, Petri, J Keith, Joung

Publikováno v: Nature biotechnology.

The CRISPR prime editor PE2 consists of a Streptococcus pyogenes Cas9 nickase (nSpCas9) fused at its C-terminus to a Moloney murine leukemia virus reverse transcriptase (MMLV-RT). Here we show that separated nSpCas9 and MMLV-RT proteins function as e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6ef1528a626858568d2d968d958e16fc
https://pubmed.ncbi.nlm.nih.gov/36163548

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Near-infrared fluorescent northern blot

Autor: Tianqi Wei, Bret R. Miller, Christopher J. Fields, Mingyi Xie, Peike Sheng

Publikováno v: RNA. 24:1871-1877

Northern blot analysis detects RNA molecules immobilized on nylon membranes through hybridization with radioactive 32P-labeled DNA or RNA oligonucleotide probes. Alternatively, nonradioactive northern blot relies on chemiluminescent reactions trigger

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2e1b7f7eccb5c78c247e96072d91907
https://doi.org/10.1261/rna.068213.118

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Northern Blot with IR Fluorescent Probes: Strategies for Probe Preparation

Autor: Peike Sheng, Christopher J. Fields, Tianqi Wei, Mingyi Xie, Bret R. Miller

Publikováno v: Bio-protocol. 9(8)

Northern blot is a molecular biology technique that can detect, quantify, and determine the molecular weight of RNA. Recently, we published a protocol utilizing near-infrared (IR) fluorescent probes in Northern blot (irNorthern). Our method is as sen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3330da4235a694f2c56b1e4f2a3c0c2f
https://pubmed.ncbi.nlm.nih.gov/31428663

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