Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Brenda Grimes"'
Autor:
Dirk Schindelhauer, Howard J. Cooke, Brenda Grimes, Niolette I. McGill, Andrew Ross, Thomas A. Ebersole, Elma Clark
Publikováno v:
Human Molecular Genetics. 9:1623-1631
Mammalian artificial chromosomes (MACs) form in HT1080 cells after transfecting linear yeast artificial chromosome constructs minimally containing competent alphoid arrays, a selectable marker and terminal human telomere repeats. Restrictions on the
Autor:
Hiroshi Masumoto, Tuneko Okazaki, Howard J. Cooke, Megumi Nakano, Kaori Saitoh, Masashi Ikeno, Brenda Grimes, Harumi Hoshino, Niolette I. McGill
Publikováno v:
Nature Biotechnology. 16:431-439
To construct a mammalian artificial chromosome (MAC), telomere repeats and selectable markers were introduced into a 100 kb yeast artificial chromosome (YAC) containing human centromeric DNA. This YAC, which has a regular repeat structure of alpha-sa
Autor:
Brenda Grimes, Brittney-Shea Herbert, Vincent H. Gattone, Heather H. Ward, Christopher J. Ward, Michael Werner, Peter C. Harris, Sandro Rossetti, Robert L. Bacallao, Elsa Bello-Reuss, Wei Min Xu, Angela Wandinger-Ness, Carrie L. Phillips, Caroline Miller
Publikováno v:
PLoS ONE, Vol 8, Iss 1, p e55191 (2013)
PLoS ONE
PLoS ONE
Autosomal dominant polycystic kidney disease (ADPKD) is associated with a variety of cellular phenotypes in renal epithelial cells. Cystic epithelia are secretory as opposed to absorptive, have higher proliferation rates in cell culture and have some
Autor:
Richard S. Hayward, Mary Logan, Helen Williamson, Brenda Grimes, Ashok Kumar, Stephen Wedgwood
Publikováno v:
Journal of Molecular Biology. 246:563-571
Most of the sigma (transcriptional initiation specificity) subunits of RNA polymerase, from a wide range of eubacteria, show strong elements of amino acid sequence similarity. There is evidence that two of the "conserved" regions, 2.4 and 4.2, are in
Autor:
Andrew Ross, Brenda Grimes, Howard J. Cooke, Thomas A. Ebersole, Nio I. McGill, Dirk Schindelhauer
We have investigated the potential of PAC-based vectors as a route to the incorporation of a gene in a mammalian artificial chromosome (MAC). Previously we demonstrated that a PAC (PAC7c5) containing alpha-satellite DNA generated mitotically stable M
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3da3e0bdf9ba48e0b2dbed8c7b91e9c
https://europepmc.org/articles/PMC1084075/
https://europepmc.org/articles/PMC1084075/
Autor:
Brenda Grimes, Megumi Nakano, Nobutaka Suzuki, Masashi Ikeno, Howard J. Cooke, Tuneko Okazaki, Hiroshi Masumoto
Publikováno v:
Chromosoma. 107(6-7)
In order to define a functional human centromere sequence, an artificial chromosome was constructed as a reproducible DNA molecule. Mammalian telomere repeats and a selectable marker were introduced into yeast artificial chromosomes (YACs) containing
Publikováno v:
Development genes and evolution. 208(9)
Differential screening was used to isolate a genomic clone, lambda89Ba located at 89B that hybridised preferentially with female cDNA. On further investigation, a 3.1-kb subfragment, 89Ba(3.1), was shown to contain a gene with male germline-specific
Autor:
Howard J. Cooke, Brenda Grimes
Publikováno v:
Human molecular genetics. 7(10)
Construction of a mammalian artificial chromosome (MAC) will develop our understanding of the requirements for normal chromosome maintenance, replication and segregation while offering the capacity for introducing genes into cells. Construction of MA
Autor:
Brenda Grimes, David Kipling, Claudia Teschke, Christine J. Farr, Niolette I. McGill, Howard J. Cooke, Patricia Malloy, Helen E. Wilson, Sally H. Cross
Publikováno v:
Genomic Instability and Immortality in Cancer ISBN: 9781461374480
The ends of linear mammalian chromosomes consist of a simple repeated sequence (T2AG3)n [reviewed in 1,2]. These terminal repeats play a role in prevention of end to end fusion and prot7ection from exonucleolytic degradation [3]. A ribonucleoprotein
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6f72fd3dc9427394e22db528a0751d39
https://doi.org/10.1007/978-1-4615-5365-6_9
https://doi.org/10.1007/978-1-4615-5365-6_9
Autor:
Kozo Makino, Brenda Grimes, Akira Ishihama, Richard A. Malloch, Nobuyuki Fujita, Richard S. Hayward, Ashok Kumar
Publikováno v:
Journal of molecular biology. 235(2)
The role of the sigma 70 subunit of Escherichia coli RNA polymerase in transcription activation by positive transcription factors was investigated. For this purpose, we constructed a nested set of E. coli rpoD deletion mutants generating carboxy-term