A multi-angular view on the impact of protein unfolding on biophysical structural data

Autor: Husniye Yigit-Gercek, Yunus Saricay, Eef H.C. Dirksen, Bregje J. de Kort

Publikováno v: Analytical biochemistry. 630

The performance of biophysical methods used for the characterization of protein higher order structure (HOS) is key to ensure reliable structural data for drug applications, as these methods are not routinely validated. To assess the analytical perfo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::048e44d5e49786cb7cad3178d0f30280
https://pubmed.ncbi.nlm.nih.gov/34389298

Potential of capillary electrophoresis with wavelength-resolved fluorescence detection for protein unfolding studies using beta-lactoglobulin B as a test compound

Autor: Bregje J. de Kort, Gerhardus J. de Jong, Govert W. Somsen

Publikováno v: Analyst, 138, 4550-4557. Royal Society of Chemistry
de Kort, B J, de Jong, G J & Somsen, G W 2013, ' Potential of capillary electrophoresis with wavelength-resolved fluorescence detection for protein unfolding studies using beta-lactoglobulin B as a test compound ', Analyst, vol. 138, pp. 4550-4557 . https://doi.org/10.1039/c3an00357d

Capillary electrophoresis (CE) with wavelength-resolved fluorescence detection (wrFlu) was evaluated for the study of protein unfolding using non-reduced and reduced β-lactoglobulin B (β-LGB) as model compounds. Protein unfolding was achieved by in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da2f15663d63b01167db0385a72f5f38
https://hdl.handle.net/1871.1/d5f65c84-0529-45be-a67a-d8a32892bec7

Lamp-based wavelength-resolved fluorescence detection for protein capillary electrophoresis: Setup and detector performance

Autor: Bregje J. de Kort, Gerhardus J. de Jong, Govert W. Somsen

Publikováno v: Electrophoresis, 31(17), 2861-2868. Wiley-VCH Verlag
de Kort, B J, de Jong, G J & Somsen, G W 2010, ' Lamp-based wavelength-resolved fluorescence detection for protein capillary electrophoresis: Setup and detector performance ', Electrophoresis, vol. 31, no. 17, pp. 2861-2868 . https://doi.org/10.1002/elps.201000246

A lamp-based fluorescence detection (Flu) system for CE was extended with a wavelength-resolved (WR) detector to allow recording of full protein emission spectra. WRFlu was achieved using a fluorescence cell that employs optical fibres to lead excita

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1333d749e9b13d855e556ca4aa04b2ee
https://doi.org/10.1002/elps.201000246

Capillary electrophoresis with lamp-based wavelength-resolved fluorescence detection for the probing of protein conformational changes

Autor: Bregje J. de Kort, Geert A. ten Kate, Govert W. Somsen, Gerhardus J. de Jong

Publikováno v: Analytical Chemistry, 83(15), 6060-6067. American Chemical Society
de Kort, B J, ten Kate, G A, de Jong, G J & Somsen, G W 2011, ' Capillary Electrophoresis with Lamp-Based Wavelength-Resolved Fluorescence Detection for the Probing of Protein Conformational Changes ', Analytical Chemistry, vol. 83, no. 15, pp. 6060-6067 . https://doi.org/10.1021/ac201136y

Native protein fluorescence spectra encompass information on protein conformation. In this study, capillary electrophoresis (CE) combined with lamp-based wavelength-resolved fluorescence detection (wrFlu) is presented as a novel tool for the analysis

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31c680546f3d226b1b01eebae99e0543
https://pubmed.ncbi.nlm.nih.gov/21699162