Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Braeden L, Butler"'
Autor:
Michelle E. Boursier, Thomas Machleidt, Christopher T. Eggers, Keith V. Wood, Robin Hurst, Kris Zimmerman, Thomas A. Kirkland, Braeden L. Butler, Rachel Friedman Ohana, Sergiy Levin
Publikováno v:
J Biol Chem
G protein-coupled receptors (GPCRs) are prominent targets to new therapeutics for a range of diseases. Comprehensive assessments of their cellular interactions with bioactive compounds, particularly in a kinetic format, are imperative to the developm
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7589f7a061f6ba269a4ec6f01033956
https://doi.org/10.1074/jbc.ra119.011952
https://doi.org/10.1074/jbc.ra119.011952
Publikováno v:
Methods in cell biology. 165
Autophagy is an important intracellular pathway for the degradation of superfluous or harmful subcellular materials, thereby playing a critical role in the maintenance of cell health under normal and stress-related conditions. Researchers interrogati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::2e0ad41d3aeaa789153d08ec06890579
https://pubmed.ncbi.nlm.nih.gov/34311870
https://pubmed.ncbi.nlm.nih.gov/34311870
Publikováno v:
Methods in Cell Biology ISBN: 9780128244876
Autophagy is an important intracellular pathway for the degradation of superfluous or harmful subcellular materials, thereby playing a critical role in the maintenance of cell health under normal and stress-related conditions. Researchers interrogati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f2ed03190d632fb62bc719fbf36262df
https://doi.org/10.1016/bs.mcb.2021.02.004
https://doi.org/10.1016/bs.mcb.2021.02.004
Autor:
Stefanie Galbán, Yong Hyun Jeon, Brittany M Bowman, James Stevenson, Katrina A Sebolt, Lisa M Sharkey, Michael Lafferty, Benjamin A Hoff, Braeden L Butler, Susan S Wigdal, Brock F Binkowski, Paul Otto, Kris Zimmerman, Gediminas Vidugiris, Lance P Encell, Frank Fan, Keith V Wood, Craig J Galbán, Brian D Ross, Alnawaz Rehemtulla
Publikováno v:
PLoS ONE, Vol 8, Iss 6, p e66248 (2013)
In addition to their degradative role in protein turnover, proteases play a key role as positive or negative regulators of signal transduction pathways and therefore their dysregulation contributes to many disease states. Regulatory roles of protease
Externí odkaz:
https://doaj.org/article/92a47bc9999a43418aeead5d0ff96642
Autor:
Brock Binkowski, Mary P. Hall, Monika G. Wood, Kristopher Zimmerman, Paul Otto, Keith V. Wood, Gediminas Vidugiris, Dieter Klaubert, Hélène A Benink, Matthew B. Robers, James Unch, Lance P. Encell, Michael P. Valley, Frank Fan, Poncho Meisenheimer, Braeden L. Butler, Thomas Machleidt, Christopher T. Eggers, Michael R. Slater
Publikováno v:
ACS Chemical Biology
Bioluminescence methodologies have been extraordinarily useful due to their high sensitivity, broad dynamic range, and operational simplicity. These capabilities have been realized largely through incremental adaptations of native enzymes and substra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b14760e5049c02c7d69b1883d21b6ca
https://doi.org/10.1021/cb3002478
https://doi.org/10.1021/cb3002478
Autor:
James J. Cali, Braeden L. Butler, Christopher T. Eggers, Ryan Kessens, Brock Binkowski, Gediminas Vidugiris, Michael R. Slater, Dan Lazar, Amani A. Gillette, Dongping Ma
Publikováno v:
Cancer Research. 78:1340-1340
The importance of autophagy in normal cell health and diseased states, including cancer, immunology and inflammation, has become quite clear. Given the growing interest in screening for autophagy modulators, we have developed a homogeneous plate-base
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d2480044cb9e9baabf009b093e7b72d0
https://doi.org/10.1158/1538-7445.am2018-1340
https://doi.org/10.1158/1538-7445.am2018-1340
Autor:
Martin Pule, Amit P. Jathoul, Audrey L. Davis, Frank Fan, Tara L. Southworth, Danielle M. Ablamsky, Braeden L. Butler, Bruce R. Branchini
Publikováno v:
Analytical Biochemistry. 396:290-297
North American firefly Photinus pyralis luciferase, which emits yellow-green light (557nm), has been adapted for a variety of applications, including gene reporter assays, whole-cell biosensor measurements, and in vivo imaging. Luciferase variants wi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c41b7ee2c23b79d9d9b9b312a4d8c31e
https://doi.org/10.1016/j.ab.2009.09.009
https://doi.org/10.1016/j.ab.2009.09.009
NanoLuc Complementation Reporter Optimized for Accurate Measurement of Protein Interactions in Cells
Autor:
Marie K. Schwinn, Thomas H. Lubben, Lance P. Encell, Thomas Machleidt, Christopher T. Eggers, Thomas A. Kirkland, Mary P. Hall, Brock Binkowski, Keith V. Wood, Braeden L. Butler, Paul Otto, Kris Zimmerman, Andrew S. Dixon, Monika G. Wood
Publikováno v:
ACS chemical biology. 11(2)
Protein-fragment complementation assays (PCAs) are widely used for investigating protein interactions. However, the fragments used are structurally compromised and have not been optimized nor thoroughly characterized for accurately assessing these in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d7c531afccfd9736db86d62401f90fa
https://pubmed.ncbi.nlm.nih.gov/26569370
https://pubmed.ncbi.nlm.nih.gov/26569370
Autor:
Julie M. Ferrell, Jinzhu Li, Alexandra C. Vrazo, Braeden L. Butler, Brock Binkowski, Adrianne E. Hontz, Kimberly A. Risma, Sarah K. Figueira
Publikováno v:
Blood. 126(8)
Growing interest in natural killer (NK) cell-based therapy for treating human cancer has made it imperative to develop new tools to measure early events in cell death. We recently demonstrated that protease-cleavable luciferase biosensors detect gran
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95aea04c49b1ac1c6679ecfcbb815405
https://pubmed.ncbi.nlm.nih.gov/26567157
https://pubmed.ncbi.nlm.nih.gov/26567157
Autor:
Keith V. Wood, Brock Binkowski, Frank Fan, Marie K. Schwinn, Robin Hurst, Thomas Machleidt, Christopher T. Eggers, Braeden L. Butler, Lance P. Encell, Mary P. Hall
Publikováno v:
Cancer Research. 77:4013-4013
Dysregulation of protein expression is a key mechanism of tumorigenesis. The most commonly used approach to monitor changes in expression is to perform SDS-PAGE, followed by immunoblotting, a labor-intensive process that requires high-quality antibod
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::172def52922110c953c1fe582fbb354b
https://doi.org/10.1158/1538-7445.am2017-4013
https://doi.org/10.1158/1538-7445.am2017-4013