Distinct mechanisms regulating mechanical force-induced Ca2+signals at the plasma membrane and the ER in human MSCs

Autor: Kim, TJ, Joo, C, Seong, J, Vafabakhsh, R, Botvinick, EL, Berns, MW, Palmer, AE, Wang, N, Ha, T, Jakobsson, E, Sun, J, Wang, Y

Publikováno v: Kim, TJ; Joo, C; Seong, J; Vafabakhsh, R; Botvinick, EL; Berns, MW; et al.(2015). Distinct mechanisms regulating mechanical force-induced Ca2+signals at the plasma membrane and the ER in human MSCs. eLife, 2015(4). doi: 10.7554/eLife.04876. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/3nn1s97b

© 2015, eLife Sciences Publications Ltd. All rights reserved. It is unclear that how subcellular organelles respond to external mechanical stimuli. Here, we investigated the molecular mechanisms by which mechanical force regulatesCa2+signaling at en

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http://www.escholarship.org/uc/item/3nn1s97b

Shrink-Film Configurable Multi-scale Wrinkles to Align Human Embryonic Stem Cells

Autor: Botvinick, EL, Chen, A, Freschauf, L, Lew, V, Sharma, H, Nguyen, D, Gopinathan, A, Fowlkes, CC, Khine, M

Publikováno v: Botvinick, EL; Chen, A; Freschauf, L; Lew, V; Sharma, H; Nguyen, D; et al.(2011). Shrink-Film Configurable Multi-scale Wrinkles to Align Human Embryonic Stem Cells. Advanced Materials. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/6gp0p3p8

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http://www.escholarship.org/uc/item/6gp0p3p8

'RoboLase’: Internet-accessible robotic laser scissors and laser tweezers microscope systems

Autor: Botvinick, EL, Shi, LZ, Berns, MW

Publikováno v: Botvinick, EL; Shi, LZ; & Berns, MW. Bozovic, V ed. (2008). 'RoboLase’: Internet-accessible robotic laser scissors and laser tweezers microscope systems. In Medical Robots. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/1rj1274t

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http://www.escholarship.org/uc/item/1rj1274t

Laser-Based Measurements in Cell Biology

Autor: Botvinick, EL, Shah, JV

Publikováno v: Botvinick, EL; & Shah, JV. (2007). Laser-Based Measurements in Cell Biology. Methods in Cell Biology, 82, 81-109. doi: 10.1016/S0091-679X(06)82003-0. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/5wc1731f

In this chapter, we review the imaging techniques and methods of molecular interrogation made possible by integrating laser light sources with microscopy. We discuss the advantages of exciting fluorescence by laser illumination and review commonly us

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A real-time single sperm tracking, laser trapping and ratiometic fluorescent imaging system

Autor: Shi, LZ, Botvinick, EL, Nascimento, J, Chandsawangbhuwana, C, Berns, MW

Publikováno v: Shi, LZ; Botvinick, EL; Nascimento, J; Chandsawangbhuwana, C; & Berns, MW. (2006). A real-time single sperm tracking, laser trapping and ratiometic fluorescent imaging system. Proceedings of SPIE-The International Society for Optical Engineering, 6326. doi: 10.1117/12.680912. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/04h3h6mf

Sperm cells from a domestic dog were treated with oxacarbocyanine DiOC2(3), a ratiometrically-encoded membrane potential fluorescent probe in order to monitor the mitochondria stored in an individual sperm's midpiece. This dye normally emits a red fl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od_______325::1e6d88270cb7eb26de28357cc6042522
http://www.escholarship.org/uc/item/04h3h6mf