Zobrazeno 1 - 10
of 71
pro vyhledávání: '"Borys, Kierdaszuk"'
Publikováno v:
Biochimie. 148:80-86
A steady-state absorption and emission spectroscopy was used to create a comprehensive work and to study the interaction of the wild type Escherichia coli purine nucleoside phosphorylase and its mutants, PNPF159Y and PNPF159A, with a potent E. coli P
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77bc22af18b4e6a1b132fa26e6f4db3d
https://doi.org/10.1016/j.biochi.2018.02.012
https://doi.org/10.1016/j.biochi.2018.02.012
Publikováno v:
Biophysical Chemistry. 230:99-108
The aim of this study is threefold: (1) augmentation of the knowledge of the E. coli PNP binding mechanism; (2) explanation of the previously observed 'lack of FRET' phenomenon and (3) an introduction of the correction (modified method) for FRET effi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1de2302559f43746c4d6ec38a815c6e
https://doi.org/10.1016/j.bpc.2017.09.001
https://doi.org/10.1016/j.bpc.2017.09.001
Autor:
Piotr Świerk, Slawomir Szymanski, Marcin Mielecki, Kamila Kowa, Włodzimierz Zagórski, Irene Kiburu, Wiesław Szeja, Krystyna Grzelak, Bogdan Lesyng, Borys Kierdaszuk, Krzysztof Krawiec, Nicole LaRonde-LeBlanc, Waldemar Priebe, Izabela Fokt
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1834:1292-1301
The RIO kinases are essential protein factors required for the synthesis of new ribosomes in eukaryotes. Conserved in archaeal organisms as well, RIO kinases are among the most ancient of protein kinases. Their exact molecular mechanisms are under in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68998a60e910e7c37d3aa64a16f3c178
https://doi.org/10.1016/j.bbapap.2013.03.012
https://doi.org/10.1016/j.bbapap.2013.03.012
Autor:
Piotr Wilk, Wojciech Rypniewski, Wojciech Rode, Adam Jarmuła, A. Dowiercial, Borys Kierdaszuk
Publikováno v:
Pteridines, Vol 24, Iss 1, Pp 93-98 (2013)
To solve the inhibition mechanism of thymidylate synthase (TS) by N4-hydroxy-dCMP (N4-OH-dCMP), crystallographic studies were undertaken. Structures of three mouse TS (mTS) complexes with the inhibitor were solved, based on crystals formed by the enz
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6115a9f43e245b3cb2d27744334f3cff
https://doi.org/10.1515/pterid-2013-0010
https://doi.org/10.1515/pterid-2013-0010
Autor:
Wojciech Rode, Piotr Maj, Piotr Wilk, Tomasz Frączyk, Jan Ludwiczak, Adam Jarmuła, Borys Kierdaszuk, Tomasz Ruman
Publikováno v:
Molecular bioSystems. 12(4)
Endogenous thymidylate synthases, isolated from tissues or cultured cells of the same specific origin, have been reported to show differing slow-binding inhibition patterns. These were reflected by biphasic or linear dependence of the inactivation ra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81bafa77cc9133fa8dc99715b5553266
https://pubmed.ncbi.nlm.nih.gov/26916840
https://pubmed.ncbi.nlm.nih.gov/26916840
Autor:
Borys Kierdaszuk
Publikováno v:
Journal of Fluorescence. 23:339-347
We examined the emission spectra and steady-state anisotropy of tyrosinate anion fluorescence with one-photon (250–310 nm), two-photon (570–620 nm) and three-photon (750–930 nm) excitation. Similar emission spectra of the neutral (pH 7.2) and a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::80783620f6f3642031db616e2fc31020
https://doi.org/10.1007/s10895-012-1152-z
https://doi.org/10.1007/s10895-012-1152-z
Autor:
Izabela Rumak, Katarzyna Gieczewska, Wiesław I. Gruszecki, Borys Kierdaszuk, Radosław Mazur, Agnieszka Mostowska, Maciej Garstka
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1797:1736-1748
We performed for the first time three-dimensional (3D) modelling of the entire chloroplast structure. Stacks of optical slices obtained by confocal laser scanning microscope (CLSM) provided a basis for construction of 3D images of individual chloropl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0c81d6309bda1444cd73884d5f43905
https://doi.org/10.1016/j.bbabio.2010.07.001
https://doi.org/10.1016/j.bbabio.2010.07.001
Autor:
Borys, Kierdaszuk
Publikováno v:
Postepy biochemii. 61(3)
Environment of human being usually contains a high number of environmental mutagens, which may modify chemically nucleic acid bases into promutagenic analogues. Hydroxylamine (NH2OH) is a strong mutagen which modifies cytosine and adenine to N4-hydro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::161329adbf3d09940b92a5db946d59da
https://pubmed.ncbi.nlm.nih.gov/26677577
https://pubmed.ncbi.nlm.nih.gov/26677577
Autor:
Bogdan Lesyng, M. W. Kalinowski, K. A. Krzyśko, Małgorzata Prokopowicz, Joanna Cieśla, M. Sobieraj, Adrian Gojdź, Borys Kierdaszuk, Adam Jarmuła
Publikováno v:
Journal of Molecular Modeling
Predicting FRET pathways in proteins using computer simulation techniques is very important for reliable interpretation of experimental data. A novel and relatively simple methodology has been developed and applied to purine nucleoside phosphorylase
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::533c55392c3d4978ee8a9c9b8d5fb305
http://europepmc.org/articles/PMC4353879
http://europepmc.org/articles/PMC4353879
Autor:
Borys Kierdaszuk, Jakub Wlodarczyk
Publikováno v:
Biophysical Chemistry. 123:146-153
Fluorescence decays in protein-ligand complexes are described by a new efficient model of continuous distribution of fluorescence lifetimes, and compared with multi-exponential models. Resulted analytical power-like decay function provides good fits
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6cf59d570caf34dece9301248b1b3f3a
https://doi.org/10.1016/j.bpc.2006.05.014
https://doi.org/10.1016/j.bpc.2006.05.014