Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Boris Grego"'
Publikováno v:
Journal of Chromatography A. 511:147-153
A method has been developed for the rapid separation of the medium-molecular-weight aplipoproteins A-IV, A-I and E by high-performance liquid chromatography. Separations were achieved using a commercially available column of very low hydrophobicity (
Autor:
K. Lin Seet, Janet Weinstock, Rosemary Chandler, Graham S. Baldwin, Michael R. Ruibira, Boris Grego
Publikováno v:
The International journal of biochemistry. 26(4)
1. A 78 kDa protein (p78) has been partially purified from washed membranes isolated from the corpus of porcine gastric mucosa. The purification was monitored by covalent cross-linking of iodinated [Nle15]-gastrin. 2. A single N-terminal sequence ext
Autor:
Rosemary Chandler, John Pedersen, Graham S. Baldwin, Boris Grego, Richard J. Simpson, Janet Weinstock, Tony Bacic, Ban-Hock Toh
Publikováno v:
Comparative biochemistry and physiology. B, Comparative biochemistry. 95(2)
1. 1. An iron-binding glycoprotein has been purified to homogeneity from porcine gastric mucosa. 2. 2. The molecular weight (80,000), amino acid composition, carbohydrate content, N-terminal amino acid sequence, tryptic map, stoichiometry of iron bin
Autor:
Richard J. Simpson, Graham S. Baldwin, Boris Grego, Francis J. Morgan, Milton T.W. Hearn, J.A. Knessel
Publikováno v:
Journal of Chromatography A. 297:21-29
Procedures for the purification of native and phosphorylated human growth hormone (hGH), S-carboxymethylated hGH, and hGH tryptic peptides, based exclusively on reversed-phase chromatography have been developed. Combinations of several volatile ion-p
Autor:
Richard J. Simpson, Boris Grego, Peter A. Stearne, Ian R. van Driel, James W. Goding, Edouard C. Nice
Publikováno v:
European Journal of Biochemistry. 148:485-491
We describe herein the use of reversed-phase high-performance liquid chromatography coupled with the novel application of short (10 cm or less) microbore columns (2 mm internal diameter) to fractionate and purify a number of tryptic peptides generate
Publikováno v:
Journal of Liquid Chromatography. 4:1725-1744
The semi-preparative separation of underivatised peptide mixtures from the tryptic digestion of thyroid and pituitary proteins has been accomplished on a 10dp spherical octadecyl-silica stationary phase, Radial Pak A/C18, packed into flexible-walled
Publikováno v:
Journal of Chromatography A. 266:89-103
The chromatographic behaviour of bovine and human thyrotropin, radiolabelled stoichiometrically with lactoperoxidase, on octadecylsilicas and other reversed-phase n -alkylsilicas has been investigated. As part of this investigation the effects of a v
Autor:
Graham S. Baldwin, Boris Grego, Francis J. Morgan, M. T. W. Hearn, J. A. Knesel, Richard J. Simpson
Publikováno v:
Proceedings of the National Academy of Sciences. 80:5276-5280
In the present study, we have demonstrated that human growth hormone (hGH) can be phosphorylated by the epidermal growth factor (EGF)-stimulated tyrosine kinase of A431 cell membranes. Phosphotyrosine was the predominant phosphoamino acid released fr
Autor:
Mitsuo Murata, Edouard C. Nice, Boris Grego, Richard J. Simpson, Fuminori Yoshizaki, Yasutomo Sugimura, Hans C. Freeman, Robert L. Moritz
Publikováno v:
European Journal of Biochemistry. 157:497-506
The complete amino acid sequence of the plastocyanin from the green alga Enteromorpha prolifera has been determined by Edman degradation of the intact molecule and fragments produced by enzymatic cleavage of the polypeptide chain with chymotrypsin, S
Publikováno v:
European Journal of Biochemistry. 165:21-29
A high-performance liquid chromatographic procedure for recovering subnanomole amounts of protein from SDS/polyacrylamide gel electroeluates in a form suitable for gas-phase sequence analysis has been developed. By a judicious choice of reversed-phas