Výsledky vyhledávání - "Boris Galunsky"

Original enzyme-catalyzed synthesis of chalcones: Utilization of hydrolase promiscuity

Autor: Boris Galunsky, Andreas Liese, Yavor Mitrev, Daniela I. Batovska, Aleksander Y Mehandzhiyski

Publikováno v: Journal of the Serbian Chemical Society, Vol 81, Iss 11, Pp 1231-1237 (2016)

An E -chalcone was obtained with very high stereoselectivity for the first time by an enzyme-catalyzed Claisen–Schmidt condensation between benzaldehyde and acetophenone. From a set of lipases, only that from hog pancreas demonstrated promiscuity,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9af497643f53e97790ced825119ff05d
http://www.doiserbia.nb.rs/img/doi/0352-5139/2016/0352-51391600069M.pdf

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A highly enantioselective aminopeptidase from sunflower seed—Kinetic studies, substrate mapping and application to biocatalytic transformations

Autor: Nikolina Stambolieva, Kiril Tishinov, Boris Galunsky, Peter Nedkov, Stanislav G. Bayryamov

Publikováno v: Journal of Molecular Catalysis B: Enzymatic. 59:106-110

The sunflower seed ( Helianthus annuus L.) major aminopeptidase primary specificity has been assessed with a number of specially designed amino acid amides and alkyl esters. Studies with amino acid 4-nitroanilides with straight alkyl side chains sugg

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::aa29f3eafbde81fb9d6d682be7f081e9
https://doi.org/10.1016/j.molcatb.2009.01.007

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Do N-terminal nucleophile hydrolases indeed have a single amino acid catalytic center?

Autor: Nicolina Stambolieva, Diana Zhiryakova, Ivaylo Ivanov, Sonya Ilieva, Boris Galunsky, Maya Guncheva

Publikováno v: FEBS Journal. 276:2589-2598

A new set of experimental kinetic data on the hydrolysis of a series of phenylacetyl p-substituted anilides catalyzed by penicillin G acylase from Escherichia coli (PGA) is presented in this article. The Hammett plot of log(k(cat,R)/k(cat,H)) versus

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ba6982eb0b9220c2cbfc054866772d6
https://doi.org/10.1111/j.1742-4658.2009.06987.x

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Purification and characterization of the sunflower seed (Helianthus annuus L.) major aminopeptidase

Autor: Boris Galunsky, Peter Nedkov, Nikolina Stambolieva, Kiril Tishinov, Svetla Petrova

Publikováno v: Acta Physiologiae Plantarum. 31:199-205

The sunflower seed (Helianthus annuus L.) major peptidase was purified to molecular homogeneity. It is an 80 kDa enzyme with pI of 4.6 and optimal activity at pH 7.5–8.0 and 45–50°C. It is a thiol-dependent aminopeptidase hydrolyzing peptides in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::bf27bed1a025804689b9b2fbb1a77de9
https://doi.org/10.1007/s11738-008-0220-0

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Application of Mixed-Mode Oasis MCX Adsorbent for Chromatographic Separation of Selenomethionine from Antarctic Krill After Enzymatic Digestion

Autor: Amir Bari Noubar, Mariana Siwek, Bernd Niemeyer, Robert Erdmann, Boris Galunsky

Publikováno v: Chromatographia. 67:305-308

The present communication describes the novel application of mixed-mode adsorbents for bifunctional chromatography. Oasis MCX mixed-mode adsorbent (Waters GmbH, Eschborn, Germany) shows a high binding capacity for Se-Met. The adsorbed selenoamino aci

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::88e2cee8e84b6abcf14e02a45c0af108
https://doi.org/10.1365/s10337-007-0494-1

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Enhancement of enzymatic digestion of Antarctic krill and successive extraction of selenium organic compounds by ultrasound treatment

Autor: Amir Bari Noubar, Jan Bergmann, Bernd Niemeyer, Mariana Siwek, Boris Galunsky

Publikováno v: Analytical and Bioanalytical Chemistry. 384:244-249

In a previous work we described the isolation of selenium organic species from Antarctic krill after enzymatic hydrolysis. In this paper we present the results of the influence of ultrasonication on the enzymatic treatment and the successive isolatio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22cc5c77b7dfa65c5b042f53c79a82e2
https://doi.org/10.1007/s00216-005-0163-x

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Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263

Autor: Jose Kaneti, Ivaylo Ivanov, Nicolina Stambolieva, Boris Galunsky, Maya Guncheva

Publikováno v: European Journal of Biochemistry. 271:2272-2279

Kinetic experiments with a substrate series of phenylacetyl-arylamides reveal that at least one polar group in the amine moiety is required for the proper orientation of the substrate in the large nucleophile-binding subsite of penicillin acylase of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7ab8bbbd85b6382f1ce0f0bf8670a903
https://doi.org/10.1111/j.1432-1033.2004.04170.x

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Fragments of pro-peptide activate mature penicillin amidase of Alcaligenes faecalis

Autor: Boris Galunsky, Volker Kasche, Zoya Ignatova

Publikováno v: European Journal of Biochemistry. 270:4721-4728

Penicillin amidase from Alcaligenes faecalis is a recently identified N-terminal nucleophile hydrolase, which possesses the highest specificity constant (kcat/Km) for the hydrolysis of benzylpenicillin compared with penicillin amidases from other sou

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d8a0806c6a1bde5c8176670cd607163d
https://doi.org/10.1046/j.1432-1033.2003.03871.x

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