Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Bonnie M. Loveless"'
Autor:
Bernard C. Courtney, Philip D. Craw, Bonnie M. Loveless, Gerald B. Howe, M. Louise M. Pitt, David A. Kulesh, David A. Norwood, Marilyn J. England, David M. Waag, John R. Lowe
Publikováno v:
Molecular and Cellular Probes. 23:127-131
Real-time PCR was used to analyze archived blood from non-human primates (NHP) and fluid samples originating from a well-controlled Q fever vaccine efficacy trial. The PCR targets were the IS1111 element and the com1 gene of Coxiella burnetii. Data f
Autor:
Ian Henderson, Ardis Barthuli, Jayanta Sinha, Sarah A. Fanders, Mark A. Gouthro, John T. Ross, Michael M. Meagher, Theresa J. Smith, Mehmet Inan, Leonard A. Smith, Rick Barent, Bonnie M. Loveless, Todd Swanson, Shinichi Taoka
Publikováno v:
Journal of Biotechnology. 127:462-474
A process was developed for production of a candidate vaccine antigen, recombinant C-terminal heavy chain fragment of the botulinum neurotoxin serotype E, rBoNTE(H(c)) in Pichia pastoris. P. pastoris strain GS115 was transformed with the rBoNTE(H(c))
Autor:
Carson Baldwin, Bonnie M. Loveless, Michelle J. Richards, David A. Norwood, Jeffrey Garrison, David A. Kulesh, Rebecca Susan Kaplan, Deanna L. Bridge, Melissa S. Frye, Deanna R. Christensen, Michelle A. Shipley, Laurie J. Hartman
Publikováno v:
Clinical Chemistry. 52:141-145
Background: Rapid detection of biological threat agents is critical for timely therapeutic administration. Fluorogenic PCR provides a rapid, sensitive, and specific tool for molecular identification of these agents. We compared the performance of ass
Autor:
Leonard P. Wasieloski, Jeffrey Garrison, Lori L Miser, Tom Larsen, Chris Hartmann, Carroll Imig, David A. Norwood, Mark J. Martinez, George V. Ludwig, Chris A. Whitehouse, Bonnie M. Loveless, David A. Kulesh, Gregory D. Huhn, Eric M. Mucker, John W. Huggins, Cynthia A. Rossi, David Miller
Publikováno v:
Laboratory Investigation. 84:1200-1208
During the summer of 2003, an outbreak of human monkeypox occurred in the Midwest region of the United States. In all, 52 rodents suspected of being infected with monkeypox virus were collected from an exotic pet dealer and from private homes. The ro
Autor:
Eric M. Mucker, John W. Huggins, Rafael Herrera, David A. Kulesh, David A. Norwood, Bonnie M. Loveless, David M. Miller, Robert O. Baker, Susan H. Zwiers, Chris Hartmann, Peter B. Jahrling, Leonard P. Wasieloski, Deanna R. Christensen
Publikováno v:
Journal of Clinical Microbiology. 42:601-609
We designed, optimized, and extensively tested several sensitive and specific real-time PCR assays for rapid detection of both smallpox and pan -orthopox virus DNAs. The assays are based on TaqMan 3′-minor groove binder chemistry and were performed
Autor:
Bonnie M. Loveless, Rebecca Susan Kaplan, Elizabeth Bode, Melissa S. Frye, William Hurtle, Deanna Bridge, David A. Kulesh, Michelle House, David A. Norwood, Jeff Garrison
Publikováno v:
Journal of Clinical Microbiology. 42:179-185
Identification of chromosomal markers for rapid detection of Bacillus anthracis is difficult because significant chromosomal homology exists among B. anthracis , Bacillus cereus , and Bacillus thuringiensis . We evaluated the bacterial gyrA gene as a
Autor:
Leonard P. Wasieloski, Bonnie M. Loveless, Anastasiya Yermakova, Deanna R. Christensen, John P. Kondig, David A. Kulesh, Henry S. Heine
Publikováno v:
Molecular and cellular probes. 24(3)
The potential for genetic modification of biological warfare agents makes rapid identification of antibiotic resistant strains critical for the implementation of suitable infection control measures. The fluorinated quinolone, ciprofloxacin, is an ant
Autor:
Mehmet Inan, Theresa J. Smith, Michael P. Dux, Jayanta Sinha, Michael M. Meagher, Todd Swanson, Rick Barent, Leonard A. Smith, Robert Webb, Mark A. Gouthro, Bonnie M. Loveless, Ian Henderson, Ardis Barthuli, John T. Ross
Publikováno v:
Protein expression and purification. 45(2)
A recombinant C-terminus heavy chain fragment from botulinum neurotoxin serotype E (BoNT/E) is proposed as a vaccine against the serotype E neurotoxin. This fragment, rBoNTE(Hc), was produced intracellular in Pichia pastoris GS115 by a three-step fer