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Autor:
Vijaykumar M. Baragi, Mahesh Mathrubutham, Howard G. Welgus, Paul J. Kuipers, Bonnie J. Shaw, Jon R. Cohen, Richard R. Renkiewicz, Srinivasa K. Rao
Publikováno v:
Matrix Biology. 19:267-273
A spectrophotometric assay using succinylated gelatin as substrate is described for measuring the catalytic activity of gelatinases. The assay is based on measurement of primary amines exposed as a result of hydrolysis of the substrate by gelatinases