Perspective: Biochemical and Physical Constraints Associated With Preparing Thin Specimens for Single-Particle Cryo-EM

Autor: Bong-Gyoon Han, Max Armstrong, Daniel A. Fletcher, Robert M. Glaeser

Publikováno v: Frontiers in Molecular Biosciences, Vol 9 (2022)

While many aspects of single-particle electron cryo-microscopy (cryo-EM) of biological macromolecules have reached a sophisticated level of development, this is not yet the case when it comes to preparing thin samples on specimen grids. As a result,

Externí odkaz: https://doaj.org/article/7e5f1f79be8b4228b327fb87768af2e5

SIMPLE ASSAY FOR ADSORPTION OF PROTEINS TO THE AIR-WATER INTERFACE

Autor: Bong-Gyoon Han, Robert M. Glaeser

A rapid assay is described, based upon the Marangoni effect, which detects the formation of a denatured-protein film at the air-water interface (AWI) of aqueous samples. This assay requires no more than a 20 μL aliquot of sample, at a protein concen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0f8db80ad39e82804ec389579b369a43
https://doi.org/10.1101/2021.08.16.456550

Microscale Fluid Behavior during Cryo-EM Sample Blotting

Autor: Bong-Gyoon Han, John Turner, Maxim Armstrong, Robert M. Glaeser, Salvador Gomez, Daniel A. Fletcher

Publikováno v: Biophysical journal, vol 118, iss 3

Blotting has been the standard technique for preparing aqueous samples for single-particle electron cryo-microscopy for over three decades. This technique removes the excess solution from a transmission electron microscope grid by pressing absorbent

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4f8a352ff3204b56e2d89f90037db40
https://resolver.caltech.edu/CaltechAUTHORS:20200102-103010460

Micro-scale fluid behavior during cryo-EM sample blotting

Autor: Bong-Gyoon Han, John Turner, Maxim Armstrong, Salvador Gomez, Robert M. Glaeser, Daniel A. Fletcher

Blotting has been the standard technique for preparing aqueous samples for single-particle electron cryo-microscopy (cryo-EM) for over three decades. This technique removes excess solution from a TEM grid by pressing absorbent filter paper against th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6868b26e2186ce501810360e9cdb5c89
https://doi.org/10.1101/791285

Factors that Influence the Formation and Stability of Thin, Cryo-EM Specimens

Autor: Roseann Csencsits, Bong-Gyoon Han, Arto Pulk, Jamie H. D. Cate, Robert M. Glaeser, Alison N. Killilea

Publikováno v: Glaeser, RM; Han, BG; Csencsits, R; Killilea, A; Pulk, A; & Cate, JHD. (2016). Factors that Influence the Formation and Stability of Thin, Cryo-EM Specimens. Biophysical Journal, 110(4), 749-755. doi: 10.1016/j.bpj.2015.07.050. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/7jj2f4zg
Biophysical journal, vol 110, iss 4

© 2016 Biophysical Society. Poor consistency of the ice thickness from one area of a cryo-electron microscope (cryo-EM) specimen grid to another, from one grid to the next, and from one type of specimen to another, motivates a reconsideration of how

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0a0bc1f0f69ef0b49de7ea559ee90fc
https://doi.org/10.1016/j.bpj.2015.07.050