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Enhancing biological signals and detection rates in single-cell RNA-seq experiments with cDNA library equalization.

Autor: Bacher R; Department of Biostatistics, University of Florida, FL, USA., Chu LF; Department of Comparative Biology and Experimental Medicine, University of Calgary, Calgary, AB, Canada.; Morgridge Institute for Research, Madison, WI, USA., Argus C; Morgridge Institute for Research, Madison, WI, USA., Bolin JM; Morgridge Institute for Research, Madison, WI, USA., Knight P; Department of Mathematics, University of Florida, FL, USA., Thomson JA; Morgridge Institute for Research, Madison, WI, USA., Stewart R; Morgridge Institute for Research, Madison, WI, USA., Kendziorski C; Department of Biostatistics, University of Wisconsin-Madison, WI, USA.

Publikováno v: Nucleic acids research [Nucleic Acids Res] 2022 Jan 25; Vol. 50 (2), pp. e12.

Reproducibility across single-cell RNA-seq protocols for spatial ordering analysis.

Autor: Seirup M; Molecular and Environmental Toxicology Program, University of Wisconsin Madison, Madison, Wisconsin, United States of America.; Morgridge Institute for Research, Madison, Wisconsin, United States of America., Chu LF; Morgridge Institute for Research, Madison, Wisconsin, United States of America., Sengupta S; Morgridge Institute for Research, Madison, Wisconsin, United States of America., Leng N; Morgridge Institute for Research, Madison, Wisconsin, United States of America.; Department of Biostatistics and Medical Informatics, University of Wisconsin Madison, Madison, Wisconsin, United States of America., Browder H; Department of Statistics, University of Florida, Gainesville, Florida, United States of America., Kapadia K; Department of Statistics, University of Florida, Gainesville, Florida, United States of America., Shafer CM; Morgridge Institute for Research, Madison, Wisconsin, United States of America., Duffin B; Morgridge Institute for Research, Madison, Wisconsin, United States of America., Elwell AL; Morgridge Institute for Research, Madison, Wisconsin, United States of America., Bolin JM; Morgridge Institute for Research, Madison, Wisconsin, United States of America., Swanson S; Morgridge Institute for Research, Madison, Wisconsin, United States of America., Stewart R; Morgridge Institute for Research, Madison, Wisconsin, United States of America., Kendziorski C; Department of Biostatistics and Medical Informatics, University of Wisconsin Madison, Madison, Wisconsin, United States of America., Thomson JA; Morgridge Institute for Research, Madison, Wisconsin, United States of America.; Department of Cell & Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, United States of America.; Department of Molecular, Cellular, & Developmental Biology, University of California Santa Barbara, Santa Barbara, California, United States of America., Bacher R; Department of Biostatistics, University of Florida, Gainesville, Florida, United States of America.

Publikováno v: PloS one [PLoS One] 2020 Sep 28; Vol. 15 (9), pp. e0239711. Date of Electronic Publication: 2020 Sep 28 (Print Publication: 2020).

Uniform neural tissue models produced on synthetic hydrogels using standard culture techniques.

Autor: Barry C; 1 Morgridge Institute for Research, Madison, WI 53705, USA., Schmitz MT; 1 Morgridge Institute for Research, Madison, WI 53705, USA., Propson NE; 1 Morgridge Institute for Research, Madison, WI 53705, USA., Hou Z; 1 Morgridge Institute for Research, Madison, WI 53705, USA.; 2 Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA (current address)., Zhang J; 1 Morgridge Institute for Research, Madison, WI 53705, USA., Nguyen BK; 1 Morgridge Institute for Research, Madison, WI 53705, USA., Bolin JM; 1 Morgridge Institute for Research, Madison, WI 53705, USA., Jiang P; 1 Morgridge Institute for Research, Madison, WI 53705, USA., McIntosh BE; 1 Morgridge Institute for Research, Madison, WI 53705, USA., Probasco MD; 1 Morgridge Institute for Research, Madison, WI 53705, USA., Swanson S; 1 Morgridge Institute for Research, Madison, WI 53705, USA., Stewart R; 1 Morgridge Institute for Research, Madison, WI 53705, USA., Thomson JA; 1 Morgridge Institute for Research, Madison, WI 53705, USA.; 3 Department of Cell & Regenerative Biology, University of Wisconsin-Madison, Madison, WI 53705, USA.; 4 Department of Molecular, Cellular, & Developmental Biology, University of California, Santa Barbara, CA 93106, USA., Schwartz MP; 5 Center for Sustainable Nanotechnology, Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA., Murphy WL; 6 Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53705, USA.; 7 Materials Science Program, University of Wisconsin-Madison, WI 53705, USA.; 8 Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, Madison, WI 53705, USA.

Publikováno v: Experimental biology and medicine (Maywood, N.J.) [Exp Biol Med (Maywood)] 2017 Nov; Vol. 242 (17), pp. 1679-1689. Date of Electronic Publication: 2017 Jun 09.