Simplified Cas13-based assays for the fast identification of SARS-CoV-2 and its variants.

Autor: Arizti-Sanz J; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Harvard-MIT Program in Health Sciences and Technology, Cambridge, MA, USA., Bradley A; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA., Zhang YB; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA., Boehm CK; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Department of Molecular Biology, Princeton University, Princeton, NJ, USA., Freije CA; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA., Grunberg ME; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Department of Molecular Biology, Princeton University, Princeton, NJ, USA., Kosoko-Thoroddsen TF; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA., Welch NL; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Program in Virology, Harvard Medical School, Boston, MA, USA., Pillai PP; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA., Mantena S; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA., Kim G; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Department of Molecular Biology, Princeton University, Princeton, NJ, USA., Uwanibe JN; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, College of Natural Sciences, Redeemer's University, Ede, Osun State, Nigeria., John OG; Department of Biological Sciences, College of Natural Sciences, Redeemer's University, Ede, Osun State, Nigeria., Eromon PE; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria., Kocher G; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institute of Health, Frederick, MD, USA., Gross R; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institute of Health, Frederick, MD, USA., Lee JS; Biotechnology Cores Facility Branch, Division of Scientific Resources, National Center for Emerging and Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA., Hensley LE; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institute of Health, Frederick, MD, USA., MacInnis BL; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Harvard T.H. Chan School of Public Health, Boston, MA, USA., Johnson J; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA., Springer M; Department of Systems Biology, Harvard Medical School, Boston, MA, USA.; Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA, USA., Happi CT; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, College of Natural Sciences, Redeemer's University, Ede, Osun State, Nigeria.; Harvard T.H. Chan School of Public Health, Boston, MA, USA., Sabeti PC; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.; Harvard T.H. Chan School of Public Health, Boston, MA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA., Myhrvold C; Department of Molecular Biology, Princeton University, Princeton, NJ, USA. cmyhrvol@princeton.edu.

Publikováno v: Nature biomedical engineering [Nat Biomed Eng] 2022 Aug; Vol. 6 (8), pp. 932-943. Date of Electronic Publication: 2022 May 30.

The Origins and Future of Sentinel: An Early-Warning System for Pandemic Preemption and Response.

Autor: Botti-Lodovico Y; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Nair P; Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA., Nosamiefan D; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria., Stremlau M; Equator Labs Incorporated, Washington, DC 20011, USA., Schaffner S; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, MA 02115, USA., Agignoae SV; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Harvard Kennedy School, Cambridge, MA 02138, USA., Aiyepada JO; Institute of Lassa Fever, Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Ajogbasile FV; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, Redeemer's University, Ede, Osun State, Nigeria., Akpede GO; Institute of Lassa Fever, Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria.; Department of Medicine, Faculty of Clinical Sciences, College of Medicine, Ambrose Alli University, Ekpoma, Edo State, Nigeria., Alhasan F; Viral Hemorrhagic Fever Program, Kenema Government Hospital, Ministry of Health and Sanitation, Kenema, Sierra Leone., Andersen KG; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA.; Scripps Research Translational Institute, La Jolla, CA 92037, USA., Asogun DA; Ambrose Alli University, Ekpoma, Edo State, Nigeria., Ayodeji OO; Federal Medical Centre, Owo, Ondo State, Nigeria., Badiane AS; Université Cheikh Anta Diop, BP 5005, Dakar, Senegal., Barnes K; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, MA 02115, USA., Bauer MR; Division of Medical Sciences, Harvard Medical School, Boston, MA 02115, USA., Bell-Kareem A; Tulane University Medical Center, New Orleans, LA 70112, USA., Benard ME; Institute of Lassa Fever, Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Benevolence EO; Institute of Lassa Fever, Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Blessing O; Institute of Lassa Fever, Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Boehm CK; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Molecular Biology, Princeton University, Princeton, NJ 08540, USA., Boisen ML; Zalgen Labs, Germantown, MD 20876, USA., Bond NG; Tulane University Medical Center, New Orleans, LA 70112, USA., Branco LM; Zalgen Labs, Germantown, MD 20876, USA., Butts MJ; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA., Carter A; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Colubri A; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; University of Massachusetts Medical School, Worcester, MA 01605, USA., Deme AB; Université Cheikh Anta Diop, BP 5005, Dakar, Senegal., DeRuff KC; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Diédhiou Y; Université Cheikh Anta Diop, BP 5005, Dakar, Senegal., Edamhande AP; Institute of Lassa Fever, Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Elhamoumi S; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Engel EJ; Tulane University Medical Center, New Orleans, LA 70112, USA., Eromon P; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria., Fallah M; Refuge Place International, Bassa Town, Lower Johnsonville, Liberia., Folarin OA; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, Redeemer's University, Ede, Osun State, Nigeria., Fry B; Fathom Information Design, Boston, MA 02114, USA., Garry R; Tulane University Medical Center, New Orleans, LA 70112, USA., Gaye A; Université Cheikh Anta Diop, BP 5005, Dakar, Senegal., Gbakie M; Viral Hemorrhagic Fever Program, Kenema Government Hospital, Ministry of Health and Sanitation, Kenema, Sierra Leone., Gevao SM; University of Sierra Leone, Freetown, Sierra Leone., Gionet G; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Gladden-Young A; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Goba A; College of Medicine and Allied Health Sciences, University of Sierra Leone, Freetown, Sierra Leone., Gomis JF; Université Cheikh Anta Diop, BP 5005, Dakar, Senegal., Happi AN; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria., Houghton M; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Ihekwuazu C; Institute of Lassa Fever, Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Iruolagbe CO; Department of Internal Medicine, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Jackson J; Dimagi, Inc., Cambridge, MA 02139, USA., Jalloh S; College of Medicine and Allied Health Sciences, University of Sierra Leone, Freetown, Sierra Leone., Johnson J; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Kanneh L; Viral Hemorrhagic Fever Program, Kenema Government Hospital, Ministry of Health and Sanitation, Kenema, Sierra Leone., Kayode A; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, Redeemer's University, Ede, Osun State, Nigeria., Kemball M; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA., Kingsley OC; Department of Medical Microbiology, Alex-Ekwueme Federal University Teaching Hospital Abakaliki, Abakaliki, Ebonyi, Nigeria., Koroma V; Viral Hemorrhagic Fever Program, Kenema Government Hospital, Ministry of Health and Sanitation, Kenema, Sierra Leone., Kotliar D; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA., Mehta S; Department of Critical Care Medicine, University of Maryland Medical Center, Baltimore, MA 21201, USA., Metsky HC; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Michael A; Institute of Lassa Fever, Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Mirhashemi ME; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Modjarrad K; Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA., Momoh M; College of Medicine and Allied Health Sciences, University of Sierra Leone, Freetown, Sierra Leone.; Eastern Polytechnic College, Kenema, Sierra Leone., Myhrvold CA; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Molecular Biology, Princeton University, Princeton, NJ 08540, USA., Naregose OG; Institute of Lassa Fever, Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Ndiaye T; Université Cheikh Anta Diop, BP 5005, Dakar, Senegal., Ndiaye M; Université Cheikh Anta Diop, BP 5005, Dakar, Senegal., Ndiaye A; Université Cheikh Anta Diop, BP 5005, Dakar, Senegal., Normandin E; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA., Odia I; Institute of Lassa Fever, Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Oguzie JU; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, Redeemer's University, Ede, Osun State, Nigeria., Okogbenin SA; Institute of Lassa Fever, Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Okokhere PO; Institute of Lassa Fever, Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria.; Department of Medicine, Faculty of Clinical Sciences, College of Medicine, Ambrose Alli University, Ekpoma, Edo State, Nigeria.; Department of Medicine, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Okolie J; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria., Olawoye IB; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, Redeemer's University, Ede, Osun State, Nigeria., Olumade TJ; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, Redeemer's University, Ede, Osun State, Nigeria., Oluniyi PE; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, Redeemer's University, Ede, Osun State, Nigeria., Omoregie O; Institute of Lassa Fever, Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria.; West African Examinations Council, Yaba, Lagos State, Nigeria., Park DJ; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Paye MF; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Petros B; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Harvard-MIT Health Sciences and Technology, Cambridge, MA 02139, USA.; Harvard/MIT MD-PhD Program, Boston, MA 02115, USA., Philippakis AA; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Priscilla A; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria., Ricks A; MASS Design Group, Boston, MA 02116, USA.; Yale School of Architecture, New Haven, CT 06511, USA., Rimoin A; Department of Epidemiology, Jonathan and Karing Fielding School of Public Health, University of California, Los Angeles, CA 90095, USA., Sandi JD; College of Medicine and Allied Health Sciences, University of Sierra Leone, Freetown, Sierra Leone., Schieffelin JS; Section of Infectious Disease, Department of Pediatrics, Tulane University School of Medicine, New Orleans, LA 70112, USA., Schreiber M; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA., Seck MC; Université Cheikh Anta Diop, BP 5005, Dakar, Senegal., Siddiqui S; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Computational and Systems Biology Program, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Siddle K; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Smither AR; Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA 70112, USA., Sy M; Université Cheikh Anta Diop, BP 5005, Dakar, Senegal., Sy N; Université Cheikh Anta Diop, BP 5005, Dakar, Senegal., Tomkins-Tinch CH; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA., Tomori O; Institute of Lassa Fever, Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Ugwu C; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, Redeemer's University, Ede, Osun State, Nigeria., Uwanibe JN; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, Redeemer's University, Ede, Osun State, Nigeria., Uyigue EA; Institute of Lassa Fever, Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Victoria DI; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Ikorodu General Hospital, Ikorodu, Lagos State, Nigeria., Vinzé A; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Vodzak ME; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA., Welch N; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Division of Medical Sciences, Harvard Medical School, Boston, MA 02115, USA., Wurie HI; College of Medicine and Allied Health Sciences, University of Sierra Leone, Freetown, Sierra Leone., Zoumarou D; Université Cheikh Anta Diop, BP 5005, Dakar, Senegal., Grant DS; Viral Hemorrhagic Fever Program, Kenema Government Hospital, Ministry of Health and Sanitation, Kenema, Sierra Leone.; College of Medicine and Allied Health Sciences, University of Sierra Leone, Freetown, Sierra Leone., Ndiaye D; Department of Biological Sciences, Redeemer's University, Ede, Osun State, Nigeria.; Université Cheikh Anta Diop, BP 5005, Dakar, Senegal., MacInnis B; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA., Sabeti PC; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.; Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, MA 02115, USA.; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114, USA.; Massachusetts Consortium on Pathogen Readiness, Boston, MA 02115, USA., Happi C; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, MA 02115, USA.; Department of Biological Sciences, Redeemer's University, Ede, Osun State, Nigeria.

Publikováno v: Viruses [Viruses] 2021 Aug 13; Vol. 13 (8). Date of Electronic Publication: 2021 Aug 13.

Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time.

Autor: Barnes KG; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. kbarnes@broadinstitute.org.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, USA. kbarnes@broadinstitute.org.; MRC-University of Glasgow Centre for Virus Research, Glasgow, UK. kbarnes@broadinstitute.org., Lachenauer AE; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. annalach@stanford.edu.; Stanford University School of Medicine, Stanford, California, USA. annalach@stanford.edu., Nitido A; Ragon Institute of MGH, MIT, and Harvard, Cambridge, Massachusetts, 02139, USA.; Ph.D. Program in Virology, Division of Medical Sciences, Harvard Medical School, Boston, Massachusetts, 02115, USA., Siddiqui S; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Computational and Systems Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA., Gross R; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA., Beitzel B; Center for Genome Sciences, The United States Army Medical Research Institute for Infectious Disease, 1425 Porter Street, Fort Detrick, Maryland, 21702, USA., Siddle KJ; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA., Freije CA; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Ph.D. Program in Virology, Division of Medical Sciences, Harvard Medical School, Boston, Massachusetts, 02115, USA., Dighero-Kemp B; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA., Mehta SB; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Division of Infectious Diseases, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA., Carter A; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Uwanibe J; African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, College of Natural Sciences, Redeemer's University, Ede, Osun State, Nigeria., Ajogbasile F; African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, College of Natural Sciences, Redeemer's University, Ede, Osun State, Nigeria., Olumade T; African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, College of Natural Sciences, Redeemer's University, Ede, Osun State, Nigeria., Odia I; Institute of Lassa Fever Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Sandi JD; African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Viral Hemorrhagic Fever Program, Kenema Government Hospital, Kenema, Sierra Leone., Momoh M; African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Viral Hemorrhagic Fever Program, Kenema Government Hospital, Kenema, Sierra Leone., Metsky HC; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Department of Electrical Engineering and Computer Science, MIT, Cambridge, Massachusetts, 02139, USA., Boehm CK; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Lin AE; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Ph.D. Program in Virology, Division of Medical Sciences, Harvard Medical School, Boston, Massachusetts, 02115, USA., Kemball M; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA., Park DJ; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Branco L; Zalgen Labs, Germantown, Maryland, USA., Boisen M; Zalgen Labs, Germantown, Maryland, USA., Sullivan B; Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, USA., Amare MF; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland, USA., Tiamiyu AB; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.; Henry M. Jackson Foundation Medical Research International, Abuja, Nigeria., Parker ZF; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland, USA., Iroezindu M; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.; Henry M. Jackson Foundation Medical Research International, Abuja, Nigeria., Grant DS; Viral Hemorrhagic Fever Program, Kenema Government Hospital, Kenema, Sierra Leone.; Ministry of Health and Sanitation, Freetown, Sierra Leone., Modjarrad K; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA., Myhrvold C; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA., Garry RF; Zalgen Labs, Germantown, Maryland, USA.; Tulane University School of Medicine, New Orleans, Los Angeles, 70112, USA., Palacios G; Center for Genome Sciences, The United States Army Medical Research Institute for Infectious Disease, 1425 Porter Street, Fort Detrick, Maryland, 21702, USA., Hensley LE; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA., Schaffner SF; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, USA.; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA., Happi CT; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, USA.; African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Department of Biological Sciences, College of Natural Sciences, Redeemer's University, Ede, Osun State, Nigeria.; Institute of Lassa Fever Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria., Colubri A; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA., Sabeti PC; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, USA.; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA.; Howard Hughes Medical Institute, Chevy Chase, Maryland, USA.

Publikováno v: Nature communications [Nat Commun] 2020 Aug 17; Vol. 11 (1), pp. 4131. Date of Electronic Publication: 2020 Aug 17.

Integrated sample inactivation, amplification, and Cas13-based detection of SARS-CoV-2.

Autor: Arizti-Sanz J; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Harvard-MIT Program in Health Sciences and Technology, Cambridge, MA, USA., Freije CA; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Program in Virology, Harvard Medical School, Boston, MA, USA., Stanton AC; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Program in Virology, Harvard Medical School, Boston, MA, USA., Boehm CK; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA., Petros BA; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Harvard-MIT Program in Health Sciences and Technology, Cambridge, MA, USA.; Harvard-MIT MD-PhD Program, Boston, MA, USA., Siddiqui S; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Computational and Systems Biology PhD program, MIT, Cambridge, MA, USA., Shaw BM; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Adams G; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA., Kosoko-Thoroddsen TF; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA., Kemball ME; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA., Gross R; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD, USA., Wronka L; National Biodefense Analysis and Countermeasures Center, Fort Detrick, MD, USA., Caviness K; National Biodefense Analysis and Countermeasures Center, Fort Detrick, MD, USA., Hensley LE; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD, USA., Bergman NH; National Biodefense Analysis and Countermeasures Center, Fort Detrick, MD, USA., MacInnis BL; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Harvard T.H. Chan School of Public Health, Boston, MA, USA., Lemieux JE; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Sabeti PC; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Harvard T.H. Chan School of Public Health, Boston, MA, USA.; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA.; Massachusetts Consortium on Pathogen Readiness, Boston, MA, USA., Myhrvold C; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.; Massachusetts Consortium on Pathogen Readiness, Boston, MA, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2020 May 28. Date of Electronic Publication: 2020 May 28.

Mature B cells accelerate wound healing after acute and chronic diabetic skin lesions.

Autor: Sîrbulescu RF; Vaccine and Immunotherapy Center, Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts., Boehm CK; Vaccine and Immunotherapy Center, Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts., Soon E; Vaccine and Immunotherapy Center, Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts., Wilks MQ; Gordon Center for Medical Imaging, Nuclear Medicine and Molecular Imaging, Radiology Department, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts., Ilieş I; Healthcare Systems Engineering Institute, Department of Mechanical and Industrial Engineering, College of Engineering, Northeastern University, Boston, Massachusetts., Yuan H; Gordon Center for Medical Imaging, Nuclear Medicine and Molecular Imaging, Radiology Department, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts., Maxner B; Vaccine and Immunotherapy Center, Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts., Chronos N; Vaccine and Immunotherapy Center, Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts., Kaittanis C; Gordon Center for Medical Imaging, Nuclear Medicine and Molecular Imaging, Radiology Department, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts., Normandin MD; Gordon Center for Medical Imaging, Nuclear Medicine and Molecular Imaging, Radiology Department, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts., El Fakhri G; Gordon Center for Medical Imaging, Nuclear Medicine and Molecular Imaging, Radiology Department, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts., Orgill DP; Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts., Sluder AE; Vaccine and Immunotherapy Center, Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts., Poznansky MC; Vaccine and Immunotherapy Center, Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.

Publikováno v: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society [Wound Repair Regen] 2017 Sep; Vol. 25 (5), pp. 774-791. Date of Electronic Publication: 2017 Oct 17.