Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Bodo Holtkamp"'
Publikováno v:
European Journal of Immunology. 13:762-766
The recognition of "self determinants" by an H-2Kk-restricted cytotoxic T (Tc) cell clone (K5) has been investigated as follows: (a) differential inhibition of cytotoxicity by several monoclonal antibodies directed to determinants on the H-2Kk molecu
Autor:
Konrad Beyreuther, Günther Karmann, Bodo Holtkamp, Matthias Cramer, Klaus Rajewsky, Susanne Proksa
Publikováno v:
Immunogenetics. 22:35-48
We have previously selected structural variants of the Kk antigen from a (C3 X D2)F1 T-cell lymphoma. Those mutants were identified by the loss of certain epitopes defined by monoclonal antibodies. The variant Kk molecule from HK13.S3 cells is no lon
Publikováno v:
Immunogenetics. 9:405-421
The expression of H-2Kk antigens in a (C3H × DBA/2)F1 lymphoma cell line growing in vitro was investigated with monoclonal antibodies specific for a public antigen of theH-2K k region (H-2.m3) in fluorescence analysis and microcytotoxicity assays an
Publikováno v:
Nature. 289:66-68
We describe here a method for the isolation of somatic cell variants that express a structurally altered gene product on their cell surface. The method makes it possible to select positively for cells of the variant phenotype which is defined by loss
Publikováno v:
European journal of immunology. 13(10)
The relationship of serologically defined determinants to determinants recognized by cytotoxic T cells on molecules encoded by the Kk gene of the murine major histocompatibility complex (H-2) has been analyzed. For this purpose we used three somatic
Publikováno v:
The EMBO journal. 2(11)
In the heterozygous T lymphoma line LDHB, variants which have lost the expression of individual H-2 class I genes are spontaneously generated in vitro at a frequency of 10(-1)-10(-2). A cell line (HK13) in which the class I gene Kk is stably expresse
Autor:
Christoph Göttlinger, Klaus Gehrke, Walter Weichel, Andreas Radbruch, Klaus Rajewsky, Bernhard Liesegang, Bodo Holtkamp, Thomas K. Stackhouse
Publikováno v:
Cytometry. 6(2)
We have modified a FACS I by addition of a tunable dye laser and an optical system for fluorescence detection that allows physically independent measurement of green and red immunofluorescence. These modifications are inexpensive and should be applic