Endogenous 2μ Plasmid Editing for Pathway Engineering in Saccharomyces cerevisiae

Autor: Bo-Xuan Zeng, Ming-Dong Yao, Wen-Hai Xiao, Yun-Zi Luo, Ying Wang, Ying-Jin Yuan

Publikováno v: Frontiers in Microbiology, Vol 12 (2021)

In Saccharomyces cerevisiae, conventional 2μ-plasmid based plasmid (pC2μ, such as pRS425) have been widely adopted in pathway engineering for multi-copy overexpression of key genes. However, the loss of partition and copy number control elements of

Externí odkaz: https://doaj.org/article/33f9c5fdab1a49329a1b07038a82f759

Metabolic engineering of Saccharomyces cerevisiae for 7-dehydrocholesterol overproduction

Autor: Xiao-Jing Guo, Wen-Hai Xiao, Ying Wang, Ming-Dong Yao, Bo-Xuan Zeng, Hong Liu, Guang-Rong Zhao, Ying-Jin Yuan

Publikováno v: Biotechnology for Biofuels, Vol 11, Iss 1, Pp 1-14 (2018)

Abstract Background 7-Dehydrocholesterol (7-DHC) has attracted increasing attentions due to its great medical value and the enlarging market demand of its ultraviolet-catalyzed product vitamin D3. Microbial production of 7-DHC from simple carbon has

Externí odkaz: https://doaj.org/article/afe7e14b02e6445a82c23f63db21466f

Metabolic Engineering of

Autor: Bo-Xuan, Zeng, Ming-Dong, Yao, Ying, Wang, Wen-Hai, Xiao, Ying-Jin, Yuan

Publikováno v: Frontiers in Bioengineering and Biotechnology

Direct bioproduction of DHAA (dihydroartemisinic acid) rather than AA (artemisinic acid), as suggested by previous work would decrease the cost of semi-biosynthesis artemisinin by eliminating the step of initial hydrogenation of AA. The major challen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::3086b7c4b95d82cadc7595fcefff4d1e
https://pubmed.ncbi.nlm.nih.gov/32258005

MOESM1 of Metabolic engineering of Saccharomyces cerevisiae for 7-dehydrocholesterol overproduction

Autor: Guo, Xiao-Jing, Xiao, Wen-Hai, Wang, Ying, Yao, Ming-Dong, Bo-Xuan Zeng, Liu, Hong, Zhao, Guang-Rong, Ying-Jin Yuan

Additional file 1: Table S1. Oligonucleotides used in this study. Table S2. The Codon-optimized sequences of DHCR24s involved in this study. Table S3 Plasmids used in this study. Figure S1. Relative transcription level of the MVA pathway genes (a–h

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::330020cd9c392676ea86c85ed4268102

'Perfect' designer chromosome V and behavior of a ring derivative

Autor: Ning Zhi Liu, Wen Zheng Zhang, Qiu Hui Lin, Ting Liu, Jing Sheng Cheng, Mei Qing Fu, Kun Yang, Ming Zhu Ding, Ran Tao, J. Andrew Martin, Yue Shen, Xia Li, Jef D. Boeke, Guo Zhen Jiang, Zhi Chao Yu, Si Chen, Wan Su, Jin Gui Liu, Jian Jun Qiao, Yan Wang, Shi Yang Liu, Wen Qian Zhang, Su Wang, Hang Xu, Shi Lei Han, Wei Liu, Ken Chen, Yi Wu, Yue Liu, Ze-Xiong Xie, Juan Zhao, Yi Lin Liu, Roy Walker, Li Xiang Song, Ye Xuan Deng, Xuya Wang, Xia Wang, Rui Guo, Leslie A. Mitchell, Joel S. Bader, Ting Ting Zhang, Ming Hua Shen, Guang Rong Zhao, Xiao Tong Wei, Xiao Ran Xu, Bing-Zhi Li, Jun Qi Zhu, Ying-Jin Yuan, Hao Xing Du, Bo Xuan Zeng, Yi Ran Wang, Bin Jia, Zheng Kuang, Shi Lan Yang, Ting Li, Lan Meng Qu, Jia Qing Zhu, Kai Ren Tian, Ping Sheng Ma, Tian Qing Song, Xue Nan Li, Guang Xin Ye, Cheng Hu, Huanming Yang, Jia Fei Lv, Wei Zhang, Yisha Luo, Qi Feng, Zhu Jin, Zhen Ning Liu, Wen Qi Ding, Fang Zhai, Xin Qi, Jin Hua Zhang, Xiao Le Wu, Yizhi Cai, Meng Zhao, Xue Jiao Guo, Xue Bai, Jun Jun Dai, Meng Long Hu, Fei Fei Li, Si Yu Xin, Xiao Na Yang, En Xu Wang, Giovanni Stracquadanio, Hui Min Liu, Lin Ting Wang, Chun-Ting Zhang, Zheng Bao Xia, Da Shuai Li, Yun Wang, Nan Jia

Publikováno v: Xie, Z-X, Li, B-Z, Mitchell, L A, Wu, Y, Qi, X, Jin, Z, Jia, B, Wang, X, Zeng, B-X, Liu, H-M, Wu, X-L, Feng, Q, Zhang, W-Q, Liu, W, Ding, M-Z, Li, X, Zhao, G-R, Qiao, J-J, Cheng, J-S, Zhao, M, Kuang, Z, Wang, X, Martin, J A, Stracquadanio, G, Yang, K, Bai, X, Zhao, J, Hu, M-L, Lin, Q-H, Zhang, W-Q, Shen, M-H, Chen, S, Su, W, Wang, E-X, Guo, R, Zhai, F, Guo, X-J, Du, H-X, Zhu, J-Q, Song, T-Q, Dai, J-J, Li, F-F, Jiang, G-Z, Han, S-L, Liu, S-Y, Yu, Z-C, Yang, X-N, Chen, K, Hu, C, Li, D-S, Jia, N, Liu, Y, Wang, L-T, Wang, S, Wei, X-T, Fu, M-Q, Qu, L-M, Xin, S-Y, Liu, T, Tian, K-R, Li, X-N, Zhang, J-H, Song, L-X, Liu, J-G, Lv, J-F, Xu, H, Tao, R, Wang, Y, Zhang, T-T, Deng, Y-X, Wang, Y-R, Li, T, Ye, G-X, Xu, X-R, Xia, Z-B, Zhang, W, Yang, S-L, Liu, Y-L, Ding, W-Q, Liu, Z-N, Zhu, J-Q, Liu, N-Z, Walker, R, Luo, Y, Wang, Y, Shen, Y, Yang, H, Cai, Y, Ma, P-S, Zhang, C-T, Bader, J S, Boeke, J D & Yuan, Y-J 2017, ' "Perfect" designer chromosome V and behavior of a ring derivative ', Science, vol. 355, no. 6329 . https://doi.org/10.1126/science.aaf4704

INTRODUCTION The Saccharomyces cerevisiae 2.0 project (Sc2.0) aims to modify the yeast genome with a series of densely spaced designer changes. Both a synthetic yeast chromosome arm (synIXR) and the entirely synthetic chromosome (synIII) function wit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e38923b67576ca15f20973c8d990e9b6
https://doi.org/10.1126/science.aaf4704