Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Blanka Kühnel"'
Publikováno v:
Scopus-Elsevier
Expression of recombinant proteins as translational fusions is commonly employed to enhance stability, increase solubility and facilitate purification of the desired protein. In general, such fusion proteins must be cleaved to release the mature prot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96d2d576fdf92ab7318c3d73dff5d19e
https://doi.org/10.1093/protein/gzg091
https://doi.org/10.1093/protein/gzg091
Publikováno v:
Journal of the American Oil Chemists' Society. 73:1533-1538
The process of immobilizing enzymes is a major cost factor in the utilization of heterogeneous catalysts on an industrial scale. We have developed a new strategy, based on plant genetic manipulation, for the production of foreign peptides associated
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::eccc1db746be247f05286b5ec57a52db
https://doi.org/10.1007/bf02523521
https://doi.org/10.1007/bf02523521
Publikováno v:
Molecular endocrinology (Baltimore, Md.). 4(12)
Analysis of the relative inducibility of an extensive series of mutant glucocorticoid response elements (GREs) defines features critical to the constitution of an active GRE. Assuming that function as a GRE reflects binding of glucocorticoid receptor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e484baa3bdb6125eefc18b3e6506a941
https://pubmed.ncbi.nlm.nih.gov/1964489
https://pubmed.ncbi.nlm.nih.gov/1964489
Publikováno v:
Protein Engineering; Oct2003, Vol. 16 Issue 10, p777, 7p
Publikováno v:
Molecular Endocrinology. 3:1270-1278
Progesterone receptor-containing T47D human breast cancer cells are responsive to progestins but fail to respond to other steroid hormones, in particular dexamethasone, because they have no measurable levels of receptors for estrogens, androgens, or
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::883fa2683e91db9c9b298a896487ff22
https://doi.org/10.1210/mend-3-8-1270
https://doi.org/10.1210/mend-3-8-1270
Publikováno v:
Nucleic Acids Research, vol. 17, no. 8, pp. 3065-78
Using linker-scanning mutagenesis we had previously identified four elements within the MMTV LTR which are necessary for transcriptional stimulation by glucocorticoid hormones. Two of them overlapped with regions to which the glucocorticoid receptor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a6638068a385358767d8021b4bcb421
https://doi.org/10.1093/nar/17.8.3065
https://doi.org/10.1093/nar/17.8.3065
Publikováno v:
Molecular Endocrinology. 3:381-391
Using crude progesterone receptor preparations from T47D human breast cancer cells, we show by immunoprecipitation assay that receptor specifically and with high affinity recognizes the hormone response element (HRE) of the mouse mammary tumor virus
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cce30b8ab3a6fc76223991df0c215423
https://doi.org/10.1210/mend-3-2-381
https://doi.org/10.1210/mend-3-2-381
Autor:
Elena Buetti, Blanka Kühnel
Publikováno v:
Journal of Molecular Biology. 190:379-389
In the proviral DNA of mouse mammary tumor virus (MMTV), sequences up to approximately equal to 200 base-pairs from the RNA start site are required for stimulation of transcription by glucocorticoid hormones in cultured cells. A total of 26 mutant pl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::729a90579792b761c5b56093c572de81
https://doi.org/10.1016/0022-2836(86)90009-4
https://doi.org/10.1016/0022-2836(86)90009-4
Publikováno v:
DNA. 8:703-713
Binding of steroid hormone receptors to specific recognition sites of hormone-inducible genes is one of the events required for hormonal regulation of gene transcription. We have employed an immunoprecipitation assay to map the interaction between un
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78d2f3b1afbbb00a1843439aa9526df9
https://doi.org/10.1089/dna.1989.8.703
https://doi.org/10.1089/dna.1989.8.703
Publikováno v:
Journal of Molecular Biology. 190:367-378
Transcription of mouse mammary tumor virus DNA is stimulated by steroid hormones. The DNA sequences involved in this regulation are located in the viral long terminal repeat between positions −200 and −50 with respect to the transcription initiat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::28aed74dfb70fa8f20a1de8749ec7485
https://doi.org/10.1016/0022-2836(86)90008-2
https://doi.org/10.1016/0022-2836(86)90008-2