Zobrazeno 1 - 10
of 59
pro vyhledávání: '"Blanca López-Méndez"'
Autor:
Francisco Tenjo-Castaño, Nicholas Sofos, Blanca López-Méndez, Luisa S. Stutzke, Anders Fuglsang, Stefano Stella, Guillermo Montoya
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
The cryo-EM structure of the type VK CRISPR-associated TnsB transposase sheds light onto RNA-guided transposition, providing new possibilities to redesign CRISPR-associated transposon systems.
Externí odkaz:
https://doaj.org/article/2a440988650d42a0ba6153e6e62f9a5d
Autor:
Rafael Molina, Stefano Stella, Mingxia Feng, Nicholas Sofos, Vykintas Jauniskis, Irina Pozdnyakova, Blanca López-Méndez, Qunxin She, Guillermo Montoya
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
Type III CRISPR-Cas RNases from the Csm and Csx families are activated by cyclic oligoadenylates (cOA). Here the authors present the cOA bound Sulfolobus islandicus Csx1 structure, which forms a hexamer and reveal an allosteric mechanism for the acti
Externí odkaz:
https://doaj.org/article/a0552bfd06e54104982c7ac8deb8d5c3
Autor:
Gulnahar B. Mortuza, Dario Hermida, Anna-Kathrine Pedersen, Sandra Segura-Bayona, Blanca López-Méndez, Pilar Redondo, Patrick Rüther, Irina Pozdnyakova, Ana M. Garrote, Inés G. Muñoz, Marina Villamor-Payà, Cristina Jauset, Jesper V. Olsen, Travis H. Stracker, Guillermo Montoya
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-17 (2018)
The Tousled-like kinase (TLKs) family belongs to a distinct branch of Ser/Thr kinases that exhibit the highest levels of activity during DNA replication. Here the authors present the crystal structure of the kinase domain from human TLK2 and propose
Externí odkaz:
https://doaj.org/article/5782a4cbd30a44858e898e8e1633bd30
Autor:
Gang Zhang, Thomas Kruse, Blanca López-Méndez, Kathrine Beck Sylvestersen, Dimitriya H. Garvanska, Simone Schopper, Michael Lund Nielsen, Jakob Nilsson
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-12 (2017)
The spindle assembly checkpoint ensures correct chromosome segregation and relies on kinetochore localization of the Bub1 and Mad1/Mad2 checkpoint proteins. Here the authors show that main function of Bub1 is to position Mad1 close to KNL1 MELT repea
Externí odkaz:
https://doaj.org/article/443aced42d614c6d811e9ee26b08fd00
Publikováno v:
PLoS Biology, Vol 7, Iss 12, p e1000257 (2009)
Proteins from halophilic organisms, which live in extreme saline conditions, have evolved to remain folded at very high ionic strengths. The surfaces of halophilic proteins show a biased amino acid composition with a high prevalence of aspartic and g
Externí odkaz:
https://doaj.org/article/2f1aa22bb6984479a8d5ebda25b356ae
Autor:
Rafael Molina, Ricardo Garcia-Martin, Blanca López-Méndez, Anne Louise Grøn Jensen, J Rafael Ciges-Tomas, Javier Marchena-Hurtado, Stefano Stella, Guillermo Montoya
Publikováno v:
Molina, R, Garcia-Martin, R, López-Méndez, B, Jensen, A L G, Ciges-Tomas, J R, Marchena-Hurtado, J, Stella, S & Montoya, G 2022, ' Molecular basis of cyclic tetra-oligoadenylate processing by small standalone CRISPR-Cas ring nucleases ', Nucleic Acids Research, vol. 50, no. 19, pp. 11199-11213 . https://doi.org/10.1093/nar/gkac923
15 pags., 7 figs., 1 tab.
Standalone ring nucleases are CRISPR ancillary proteins, which downregulate the immune response of Type III CRISPR-Cas systems by cleaving cyclic oligoadenylates (cA) second messengers. Two genes with this function have
Standalone ring nucleases are CRISPR ancillary proteins, which downregulate the immune response of Type III CRISPR-Cas systems by cleaving cyclic oligoadenylates (cA) second messengers. Two genes with this function have
Autor:
Lei Wang, Thomas Kruse, Blanca López-Méndez, Yuqing Zhang, Chunlin Song, Lei Zhu, Bing Li, Jing Fang, Zhimin Lu, Jakob Nilsson, Gang Zhang
Publikováno v:
Wang, L, Kruse, T, López-Méndez, B, Zhang, Y, Song, C, Zhu, L, Li, B, Fang, J, Lu, Z, Nilsson, J & Zhang, G 2023, ' Spatial separation of phosphatase and kinase activity within the Bub complex is required for proper mitosis ', Journal of Molecular Cell Biology, vol. 14, no. 11 . https://doi.org/10.1093/jmcb/mjac062
The Bub1 and BubR1 kinetochore proteins support proper chromosome segregation and mitotic checkpoint activity. Bub1 and BubR1 are paralogs with Bub1 being a kinase, while BubR1 localizes the PP2A-B56 protein phosphatase to kinetochores in humans. Whe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54f1d22139b6487f1b3ccfaff078544a
https://curis.ku.dk/ws/files/348203017/mjac062.pdf
https://curis.ku.dk/ws/files/348203017/mjac062.pdf
Autor:
Rafael Molina, Anne Louise Grøn Jensen, Stefano Stella, Guillermo Montoya, Blanca López-Méndez, Javier Marchena-Hurtado
Publikováno v:
Nucleic Acids Research
Molina Monterrubio, R A, Jensen, A L G, Marchena-Hurtado, J, López-Méndez, B, Stella, S & Montoya, G 2021, ' Structural basis of cyclic oligoadenylate degradation by ancillary Type III CRISPR-Cas ring nucleases ', Nucleic Acids Research, vol. 49, no. 21, pp. 12577-12590 . https://doi.org/10.1093/nar/gkab1130
Molina Monterrubio, R A, Jensen, A L G, Marchena-Hurtado, J, López-Méndez, B, Stella, S & Montoya, G 2021, ' Structural basis of cyclic oligoadenylate degradation by ancillary Type III CRISPR-Cas ring nucleases ', Nucleic Acids Research, vol. 49, no. 21, pp. 12577-12590 . https://doi.org/10.1093/nar/gkab1130
Type III CRISPR-Cas effector systems detect foreign RNA triggering DNA and RNA cleavage and synthesizing cyclic oligoadenylate molecules (cA) in their Cas10 subunit. cAs act as a second messenger activating auxiliary nucleases, leading to an indiscri
Autor:
Bertrand Raynal, Maria Garcia-Alai, Kim Remans, Stefan H. Knauer, Stephan Uebel, Ario de Marco, Mario Lebendiker, Annabel H. A. Parret, André Matagne, Blanca López-Méndez, Nick Berrow
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-4 (2021)
de Marco, A, Berrow, N, Lebendiker, M, Garcia-Alai, M, Knauer, S H, Lopez-Mendez, B, Matagne, A, Parret, A, Remans, K, Uebel, S & Raynal, B 2021, ' Quality control of protein reagents for the improvement of research data reproducibility ', Nature Communications, vol. 12, no. 1, 2795 . https://doi.org/10.1038/s41467-021-23167-z
Nature Communications
Nature Communications, 2021, 12 (1), pp.2795. ⟨10.1038/s41467-021-23167-z⟩
de Marco, A, Berrow, N, Lebendiker, M, Garcia-Alai, M, Knauer, S H, Lopez-Mendez, B, Matagne, A, Parret, A, Remans, K, Uebel, S & Raynal, B 2021, ' Quality control of protein reagents for the improvement of research data reproducibility ', Nature Communications, vol. 12, no. 1, 2795 . https://doi.org/10.1038/s41467-021-23167-z
Nature Communications
Nature Communications, 2021, 12 (1), pp.2795. ⟨10.1038/s41467-021-23167-z⟩
Proteins and peptides are amongst the most widely used research reagents but often their quality is inadequate and can result in poor data reproducibility. Here we propose a simple set of guidelines that, when correctly applied to protein reagents sh
Publikováno v:
European Biophysics Journal. 50:653-660
NanoTemper Monolith instruments have gained enormous popularity for measuring molecular interactions both in academia and industry. The underlying technology has been extensively reviewed along with its assumptions, limitations, and applications (Sch