Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Bjarne Stoffer"'
Autor:
Jette S. Kastrup, Bjarne Stoffer, Poul Baad Rasmussen, Anja K. Pedersen, Hans Flodgaard, Lars Fogh Iversen, Søren E. Bjørn, Viggo Linde, Ingrid Kjøller Larsen
Publikováno v:
Proteins: Structure, Function, and Bioinformatics. 42:442-451
Heparin binding protein (HBP) is an inactive serine protease homologue with important implications in host defense during infections and inflammations. Two mutants of human HBP, [R23S,F25E]HBP and [G175Q]HBP, have been produced to investigate structu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6e8b7108432adc2ecfdf0c4a37776f34
https://doi.org/10.1002/1097-0134(20010301)42:4<442::aid-prot30>3.0.co
https://doi.org/10.1002/1097-0134(20010301)42:4<442::aid-prot30>3.0.co
Autor:
Dale R. Cameron, Raymond U. Lemieux, Birte Svensson, Torben P. Frandsen, Monica M. Palcic, Mimi Bach, Bjarne Stoffer, Ulrike Spohr
Publikováno v:
Canadian Journal of Chemistry. 74:319-335
A recently developed technique for the probing of the combining sites of lectins and antibodies, to establish the structure of the epitope that is involved in the binding of an oligosaccharide, is used to study the binding of methyl α-isomaltoside b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ef5c6bfbe1da960603a426ff162a0cf2
https://doi.org/10.1139/v96-036
https://doi.org/10.1139/v96-036
Publikováno v:
Biochemistry. 35:8696-8704
Rational protein engineering based on three-dimensional structure, sequence alignment, and previous mutational analysis served to increase thermostability and modulate bond-type specificity in glucoamylase from Aspergillus awamori. The single free cy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::139ca22cb0ad2b83a928d1febf413cd8
https://doi.org/10.1021/bi960241c
https://doi.org/10.1021/bi960241c
Publikováno v:
Biochemistry. 35:15009-15018
Glucoamylase (1,4-alpha-glucan glucohydrolase, EC 3.2.1.3) from Aspergillus, of which the 3D structure is known, releases beta-D-glucose from the non-reducing ends of starch and other related oligo and polysaccharides, cleaving the alpha-1,4-bond pos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7ad10ae3292241359ae8ddd9c098837
https://doi.org/10.1021/bi9608323
https://doi.org/10.1021/bi9608323
Publikováno v:
Biochemistry. 34:10153-10161
We have investigated the binding of mutant forms of glucoamylase from Aspergillus niger to the inhibitors 1-deoxynojirimycin and acarbose. The mutants studied comprise a group of single amino acid replacements in conserved regions near the active sit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::647ca36f46286c5f197253ea3f43621f
https://doi.org/10.1021/bi00032a008
https://doi.org/10.1021/bi00032a008
Autor:
Torben P. Frandsen, Michael R. Sierks, Jan Lehmbeck, Richard B. Honzatko, Birte Svensson, Bjarne Stoffer, Claude Dupont
Publikováno v:
Biochemistry. 33:13808-13816
Replacement of the catalytic base Glu400 by glutamine in glucoamylase from Aspergillus niger affects both substrates ground-state binding and transition-state stabilization. Compared to those of the wild-type enzyme, Km values for maltose and maltohe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff9fd5c038091b18a94f3d0c70d7c1e8
https://doi.org/10.1021/bi00250a035
https://doi.org/10.1021/bi00250a035
Autor:
David E. Metzler, Alexander E. Aleshin, Birte Svensson, Carol M. Metzler, Trine Christensen, Bjarne Stoffer, Robert D. Scott, Kirill N. Neustroev, Leonid M. Firsov
Publikováno v:
European Journal of Biochemistry. 223:293-302
1H-NMR spectra have been recorded for glucoamylases I and II from Aspergillus awamori var. X100 and from A. niger in the 9-15-ppm region. At least 17 distinct peaks, many of them arising from single protons, are observed. These are designated A-Q, A
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b037bdee36296c16a372783ff168969a
https://doi.org/10.1111/j.1432-1033.1994.tb18994.x
https://doi.org/10.1111/j.1432-1033.1994.tb18994.x
Autor:
Torben P. Frandsen, B. Mario Pinto, Bjarne Stoffer, Birte Svensson, Margaret A. Johnson, Karla D. Randell
Publikováno v:
ChemInform. 31
The synthesis of a series of 5-thio- d -glucopyranosylarylamines by reaction of 5-thio- d -glucopyranose pentaacetate with the corresponding arylamine and mercuric chloride catalyst is reported. The products were obtained as anomeric mixtures of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2127201890949f8c79852dc438f6085a
https://doi.org/10.1002/chin.200015177
https://doi.org/10.1002/chin.200015177
Publikováno v:
Biochemistry. 39(2)
Transferred nuclear Overhauser effect (trNOE) experiments have been performed to investigate the conformations of the competitive inhibitors, methyl 5'-thio-4-N-alpha-maltoside 3a and methyl 5'-thio-4-S-alpha-maltoside 4 when bound to the catalytic s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::431ffb33a160a804e46250a5ea449c4c
https://pubmed.ncbi.nlm.nih.gov/10630989
https://pubmed.ncbi.nlm.nih.gov/10630989
Publikováno v:
Technical University of Denmark Orbit
Presteady and steady-state kinetic results on the interactions of a wild-type, and the mutant glucoamylases Trp52-->Phe and Trp317-->Phe, from Aspergillus niger with maltose, maltotriose and maltotetraose have been obtained and analyzed. The results
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d1b4012faeacca5fd0899b24e69603e
https://pubmed.ncbi.nlm.nih.gov/9461285
https://pubmed.ncbi.nlm.nih.gov/9461285