Plasmid DNA molecules complexed with cationic liposomes are protected from degradation by nucleases and shearing by aerosolisation

Autor: Crook K, Gerry McLachlan, Bj, Stevenson, Dj, Porteous

Publikováno v: Europe PubMed Central

The cationic liposome DOTAP was complexed with plasmid DNA encoding beta-galactosidase in various ratios. As the concentration of DOTAP increased, the DNA became increasingly refractory to staining with ethidium bromide, presumably because the DNA wa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::6aff15ec230cbcfc65f3e107ebb58f19
https://pubmed.ncbi.nlm.nih.gov/8875234

Laboratory and clinical studies in support of cystic fibrosis gene therapy using pCMV-CFTR-DOTAP

Autor: McLachlan G, Ling Pei Ho, Davidson-Smith H, Samways J, Davidson H, Bj, Stevenson, Ad, Carothers, Ew, Alton, Pg, Middleton, Sn, Smith, Kallmeyer G, Michaelis U, Dj, Porteous

Publikováno v: Europe PubMed Central

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::4ddba013d17b89f6ab418eebfc528673
http://europepmc.org/abstract/med/8986438

Evaluation in vitro and in vivo of cationic liposome-expression construct complexes for cystic fibrosis gene therapy

Autor: Gerry McLachlan, Dj, Davidson, Bj, Stevenson, Dickinson P, Davidson-Smith H, Jr, Dorin, Dj, Porteous

Publikováno v: Europe PubMed Central

We have tested the cationic liposome N-(1-(2,3-dioleoyloxy)propyl)-N,N,N-trimethyl-ammoniummethylsul phate, (DOTAP), for gene delivery in vitro and in vivo with a view to clinical use in gene therapy for cystic fibrosis. Delivery of lacZ cDNA-DOTAP c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::d92b92f7ce6d67beebe0269b24ed815a
http://europepmc.org/abstract/med/8548550

A comprehensive proteogenomic pipeline for neoantigen discovery to advance personalized cancer immunotherapy.

Autor: Huber F; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland.; Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland., Arnaud M; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland., Stevenson BJ; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland.; SIB Swiss Institute of Bioinformatics, Quartier Sorge, Bâtiment Amphipôle, Lausanne, Switzerland., Michaux J; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland.; Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland., Benedetti F; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland., Thevenet J; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland., Bobisse S; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland., Chiffelle J; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland., Gehert T; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland., Müller M; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland.; SIB Swiss Institute of Bioinformatics, Quartier Sorge, Bâtiment Amphipôle, Lausanne, Switzerland., Pak H; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland.; Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland., Krämer AI; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland.; Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland., Altimiras ER; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland.; Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland., Racle J; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland.; SIB Swiss Institute of Bioinformatics, Quartier Sorge, Bâtiment Amphipôle, Lausanne, Switzerland., Taillandier-Coindard M; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland.; Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland., Muehlethaler K; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland., Auger A; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland., Saugy D; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland., Murgues B; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland., Benyagoub A; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland., Gfeller D; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland.; SIB Swiss Institute of Bioinformatics, Quartier Sorge, Bâtiment Amphipôle, Lausanne, Switzerland., Laniti DD; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland., Kandalaft L; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland.; Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland., Rodrigo BN; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland., Bouchaab H; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Department of Medical Oncology, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland., Tissot S; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland., Coukos G; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland., Harari A; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.; AGORA Cancer Research Center, Lausanne, Switzerland., Bassani-Sternberg M; Department of Oncology, University of Lausanne (UNIL) and Lausanne University Hospital (CHUV), Lausanne, Switzerland. michal.bassani@chuv.ch.; Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland. michal.bassani@chuv.ch.; AGORA Cancer Research Center, Lausanne, Switzerland. michal.bassani@chuv.ch.; Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland. michal.bassani@chuv.ch.

Publikováno v: Nature biotechnology [Nat Biotechnol] 2024 Oct 11. Date of Electronic Publication: 2024 Oct 11.

Machine learning methods and harmonized datasets improve immunogenic neoantigen prediction.

Autor: Müller M; Ludwig Institute for Cancer Research, University of Lausanne, Agora Center Bugnon 25A, 1005 Lausanne, Switzerland; Department of Oncology, Centre hospitalier universitaire vaudois (CHUV), Rue du Bugnon 46, 1005 Lausanne, Switzerland; Agora Cancer Research Centre, 1011 Lausanne, Switzerland; SIB Swiss Institute of Bioinformatics, Quartier Sorge, Bâtiment Amphipôle, 1015 Lausanne, Switzerland. Electronic address: markus.muller@chuv.ch., Huber F; Ludwig Institute for Cancer Research, University of Lausanne, Agora Center Bugnon 25A, 1005 Lausanne, Switzerland; Department of Oncology, Centre hospitalier universitaire vaudois (CHUV), Rue du Bugnon 46, 1005 Lausanne, Switzerland; Agora Cancer Research Centre, 1011 Lausanne, Switzerland., Arnaud M; Ludwig Institute for Cancer Research, University of Lausanne, Agora Center Bugnon 25A, 1005 Lausanne, Switzerland; Department of Oncology, Centre hospitalier universitaire vaudois (CHUV), Rue du Bugnon 46, 1005 Lausanne, Switzerland; Agora Cancer Research Centre, 1011 Lausanne, Switzerland., Kraemer AI; Ludwig Institute for Cancer Research, University of Lausanne, Agora Center Bugnon 25A, 1005 Lausanne, Switzerland; Department of Oncology, Centre hospitalier universitaire vaudois (CHUV), Rue du Bugnon 46, 1005 Lausanne, Switzerland; Agora Cancer Research Centre, 1011 Lausanne, Switzerland., Altimiras ER; Ludwig Institute for Cancer Research, University of Lausanne, Agora Center Bugnon 25A, 1005 Lausanne, Switzerland; Department of Oncology, Centre hospitalier universitaire vaudois (CHUV), Rue du Bugnon 46, 1005 Lausanne, Switzerland; Agora Cancer Research Centre, 1011 Lausanne, Switzerland., Michaux J; Ludwig Institute for Cancer Research, University of Lausanne, Agora Center Bugnon 25A, 1005 Lausanne, Switzerland; Department of Oncology, Centre hospitalier universitaire vaudois (CHUV), Rue du Bugnon 46, 1005 Lausanne, Switzerland; Agora Cancer Research Centre, 1011 Lausanne, Switzerland., Taillandier-Coindard M; Ludwig Institute for Cancer Research, University of Lausanne, Agora Center Bugnon 25A, 1005 Lausanne, Switzerland; Department of Oncology, Centre hospitalier universitaire vaudois (CHUV), Rue du Bugnon 46, 1005 Lausanne, Switzerland; Agora Cancer Research Centre, 1011 Lausanne, Switzerland., Chiffelle J; Ludwig Institute for Cancer Research, University of Lausanne, Agora Center Bugnon 25A, 1005 Lausanne, Switzerland; Department of Oncology, Centre hospitalier universitaire vaudois (CHUV), Rue du Bugnon 46, 1005 Lausanne, Switzerland; Agora Cancer Research Centre, 1011 Lausanne, Switzerland., Murgues B; Ludwig Institute for Cancer Research, University of Lausanne, Agora Center Bugnon 25A, 1005 Lausanne, Switzerland; Department of Oncology, Centre hospitalier universitaire vaudois (CHUV), Rue du Bugnon 46, 1005 Lausanne, Switzerland; Agora Cancer Research Centre, 1011 Lausanne, Switzerland., Gehret T; Ludwig Institute for Cancer Research, University of Lausanne, Agora Center Bugnon 25A, 1005 Lausanne, Switzerland; Department of Oncology, Centre hospitalier universitaire vaudois (CHUV), Rue du Bugnon 46, 1005 Lausanne, Switzerland; Agora Cancer Research Centre, 1011 Lausanne, Switzerland., Auger A; Ludwig Institute for Cancer Research, University of Lausanne, Agora Center Bugnon 25A, 1005 Lausanne, Switzerland; Department of Oncology, Centre hospitalier universitaire vaudois (CHUV), Rue du Bugnon 46, 1005 Lausanne, Switzerland; Agora Cancer Research Centre, 1011 Lausanne, Switzerland., Stevenson BJ; Agora Cancer Research Centre, 1011 Lausanne, Switzerland; SIB Swiss Institute of Bioinformatics, Quartier Sorge, Bâtiment Amphipôle, 1015 Lausanne, Switzerland., Coukos G; Ludwig Institute for Cancer Research, University of Lausanne, Agora Center Bugnon 25A, 1005 Lausanne, Switzerland; Department of Oncology, Centre hospitalier universitaire vaudois (CHUV), Rue du Bugnon 46, 1005 Lausanne, Switzerland; Agora Cancer Research Centre, 1011 Lausanne, Switzerland; Center of Experimental Therapeutics, Department of Oncology, Centre hospitalier universitaire vaudois (CHUV), Rue du Bugnon 46, 1005 Lausanne, Switzerland., Harari A; Ludwig Institute for Cancer Research, University of Lausanne, Agora Center Bugnon 25A, 1005 Lausanne, Switzerland; Department of Oncology, Centre hospitalier universitaire vaudois (CHUV), Rue du Bugnon 46, 1005 Lausanne, Switzerland; Agora Cancer Research Centre, 1011 Lausanne, Switzerland; Center of Experimental Therapeutics, Department of Oncology, Centre hospitalier universitaire vaudois (CHUV), Rue du Bugnon 46, 1005 Lausanne, Switzerland., Bassani-Sternberg M; Ludwig Institute for Cancer Research, University of Lausanne, Agora Center Bugnon 25A, 1005 Lausanne, Switzerland; Department of Oncology, Centre hospitalier universitaire vaudois (CHUV), Rue du Bugnon 46, 1005 Lausanne, Switzerland; Agora Cancer Research Centre, 1011 Lausanne, Switzerland; Center of Experimental Therapeutics, Department of Oncology, Centre hospitalier universitaire vaudois (CHUV), Rue du Bugnon 46, 1005 Lausanne, Switzerland. Electronic address: michal.bassani@chuv.ch.

Publikováno v: Immunity [Immunity] 2023 Nov 14; Vol. 56 (11), pp. 2650-2663.e6. Date of Electronic Publication: 2023 Oct 09.