Comparison of Isomerase and Weimberg Pathway for γ-PGA Production From Xylose by Engineered Bacillus subtilis

Autor: Birthe Halmschlag, Kyra Hoffmann, René Hanke, Sastia P. Putri, Eiichiro Fukusaki, Jochen Büchs, Lars M. Blank

Publikováno v: Frontiers in Bioengineering and Biotechnology, Vol 7 (2020)

The production of poly-γ-glutamic acid (γ-PGA), a biopolymer consisting of D- and L-glutamic acid monomers, currently relies on L-glutamate, or citrate as carbon substrates. Here we aimed at using plant biomass-derived substrates such as xylose. γ

Externí odkaz: https://doaj.org/article/09b0aa08aea34e30ae620a978b8dc059

Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of Bacillus subtilis with the phosphate‐starvation inducible promoter P pst

Autor: Kyra Hoffmann, Birthe Halmschlag, Simon Briel, Michaela Sieben, Sastia Putri, Eiichiro Fukusaki, Lars M. Blank, Jochen Büchs

Publikováno v: Biotechnology Progress. 39

Poly-γ-glutamic acid (γ-PGA) is a biopolymer with a wide range of applications, mainly produced using Bacillus strains. The formation and concomitant secretion of γ-PGA increases the culture broth viscosity, while enzymatic depolymerisation and de

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae8d2e2d2b869397c96f321cbb756062
https://doi.org/10.1002/btpr.3293

Poly-γ-glutamic acid production by Bacillus subtilis 168 using glucose as the sole carbon source: A metabolomic analysis

Autor: Eiichiro Fukusaki, Lars M. Blank, Birthe Halmschlag, Sastia Prama Putri

Publikováno v: Journal of Bioscience and Bioengineering. 130:272-282

The industrially relevant biopolymer poly-γ-glutamic acid (γ-PGA) is commonly synthesized using glycerol, citrate, and glutamic acid as carbon sources. In this study, two strains capable of utilizing glucose as sole carbon source for γ-PGA synthes

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49ffd1a0b5593f8d080b3e17917a2bb6
https://doi.org/10.1016/j.jbiosc.2020.04.011

Tailor-made poly-γ-glutamic acid production

Autor: Eiichiro Fukusaki, Birthe Halmschlag, Sastia Prama Putri, Lars M. Blank, Xenia Steurer

Publikováno v: Metabolic Engineering. 55:239-248

Poly-γ-glutamic acid (γ-PGA), which is produced by several Bacillus species, is a chiral biopolymer composed of D- and L-glutamate monomers and has various industrial applications. However, synthesized γ-PGA exhibits great structural diversity, an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eef40966033f01659b5b478c64235928
https://doi.org/10.1016/j.ymben.2019.07.009

Identification of Key Metabolites in Poly-γ-Glutamic Acid Production by Tuning γ-PGA Synthetase Expression

Autor: Sastia Prama Putri, Eiichiro Fukusaki, Lars M. Blank, Birthe Halmschlag

Publikováno v: Frontiers in Bioengineering and Biotechnology 8, 38 (2020). doi:10.3389/fbioe.2020.00038 special issue: "Pathway, genetic and process engineering of microbes for biopolymer synthesis"
Frontiers in Bioengineering and Biotechnology
Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)

Frontiers in Bioengineering and Biotechnology 8, 38 (2020). doi:10.3389/fbioe.2020.00038
Published by Frontiers Media, Lausanne

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93479bd617f24971aa539d26ede40f82
https://publications.rwth-aachen.de/record/787483