Zobrazeno 1 - 10
of 26
pro vyhledávání: '"Birger Anspach"'
Publikováno v:
Journal of Membrane Science. 207:253-264
The purification of immunoglobulins was studied by comparing 10 different affinity membranes, prepared by coupling various affinity ligands to different microfiltration membranes. Membranes carrying the synthetic peptide TG19318, histidine, the thiop
Publikováno v:
Journal of Computer-Aided Molecular Design. 16:917-934
Lactoferrin (Lf) is a non-heme, iron binding protein present in many physiological fluids of vertebrates where its main role is the microbicidal activity. It has been isolated by different methods, including dye-affinity chromatography. Red HE-3B is
Publikováno v:
The Canadian Journal of Chemical Engineering. 77:921-930
The adsorption of endotoxin from E.coli O 111:B4 and bovine serum albumin on membrane adsorbers with the ligands polymyxin B, L-histidine, deoxycholate, poly(L-lysine), poly(ethyleneimine) and diethylaminoethyl was studied under equilibrium and dynam
Autor:
Oliver Cromwell, Stefanie Kischnick, Walter Keller, Roland Suck, F. Birger Anspach, Bernhard H. F. Weber, Helmut Fiebig, Petra Verdino, Ernst A. Sanders
Publikováno v:
Protein expression and purification. 47(2)
A process for bacterial expression and purification of the recombinant major wasp allergen Antigen 5 (Ves v 5) was developed to produce protein for diagnostic and therapeutic applications for type 1 allergic diseases. Special attention was focused on
Autor:
Friedrich Birger Anspach
Publikováno v:
Journal of Chromatography Library ISBN: 9780444511072
Summary Among the classical techniques for protein purification, affinity chromatography allows a several-fold purification from dilute preparations in a single step. At this time, the user can choose among a large variety of sorbents with different
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e7c28b7870712976083d372f4daadf3b
https://doi.org/10.1016/s0301-4770(04)80009-5
https://doi.org/10.1016/s0301-4770(04)80009-5
Autor:
F. Birger Anspach, Leda R. Castilho
Publikováno v:
Biotechnology and bioengineering. 83(5)
In the present work, a rotating disk filter was designed for mammalian cell separation with the aim of avoiding both cell damage and membrane fouling. Different geometric and operational variables of the rotating disk filter were studied using comput
Publikováno v:
Journal of computer-aided molecular design. 16(12)
Lactoferrin (Lf) is a non-heme, iron binding protein present in many physiological fluids of vertebrates where its main role is the microbicidal activity. It has been isolated by different methods, including dye-affinity chromatography. Red HE-3B is
Publikováno v:
Biotechnology progress. 18(4)
In the present work, a dynamic filter was employed to develop an integrated perfusion/purification process. A recombinant CHO cell line producing a human anti-HIV IgG was employed in the experiments. In the first part of this work, the dynamic filter
Publikováno v:
Biotechnology and bioengineering. 78(7)
Controlled shear affinity filtration (CSAF) integrates animal cell separation and product isolation in a single unit operation through the use of a specifically designed rotating disk filter with incorporated membrane chromatography column. Because o