A Novel Bacteriophage with Broad Host Range against Clostridioides difficile Ribotype 078 Supports SlpA as the Likely Phage Receptor.

Autor: Whittle MJ; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, The University of Nottinghamgrid.4563.4, Nottingham, United Kingdom., Bilverstone TW; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, The University of Nottinghamgrid.4563.4, Nottingham, United Kingdom., van Esveld RJ; Faculty of Medicine, Leiden University Medical Centre, the Netherlands., Lücke AC; Hannover Medical School, Hannover, Germany., Lister MM; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, The University of Nottinghamgrid.4563.4, Nottingham, United Kingdom.; NIHR Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust and The University of Nottinghamgrid.4563.4, Nottingham, United Kingdom., Kuehne SA; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, The University of Nottinghamgrid.4563.4, Nottingham, United Kingdom.; NIHR Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust and The University of Nottinghamgrid.4563.4, Nottingham, United Kingdom., Minton NP; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, The University of Nottinghamgrid.4563.4, Nottingham, United Kingdom.; NIHR Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust and The University of Nottinghamgrid.4563.4, Nottingham, United Kingdom.

Publikováno v: Microbiology spectrum [Microbiol Spectr] 2022 Feb 23; Vol. 10 (1), pp. e0229521. Date of Electronic Publication: 2022 Feb 02.

The glucosyltransferase activity of C. difficile Toxin B is required for disease pathogenesis.

Autor: Bilverstone TW; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, United kingdom., Garland M; Cancer Biology Program, Stanford School of Medicine, Stanford, CA, United States of America.; Department of Pathology, Stanford School of Medicine, Stanford, CA, United States of America., Cave RJ; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, United kingdom., Kelly ML; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, United kingdom., Tholen M; Department of Pathology, Stanford School of Medicine, Stanford, CA, United States of America., Bouley DM; Department of Comparative Medicine, Stanford School of Medicine, Stanford, CA, United States of America., Kaye P; Department of Histopathology, Nottingham University Hospitals and University of Nottingham NDDC NIHR BRC, Nottingham, United Kingdom., Minton NP; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, United kingdom.; NIHR Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust and the University of Nottingham, Nottingham, United Kingdom., Bogyo M; Department of Pathology, Stanford School of Medicine, Stanford, CA, United States of America.; Department of Microbiology and Immunology, Stanford School of Medicine, Stanford, CA, United States of America., Kuehne SA; Oral Microbiology Group, School of Dentistry and Institute of Microbiology and Infection, College of Medical and Dental Sciences, The University of Birmingham, Birmingham, United Kingdom., Melnyk RA; Department of Biochemistry, University of Toronto, and Program in Molecular Medicine, The Hospital for Sick Children, Toronto, ON, Canada.

Publikováno v: PLoS pathogens [PLoS Pathog] 2020 Sep 22; Vol. 16 (9), pp. e1008852. Date of Electronic Publication: 2020 Sep 22 (Print Publication: 2020).

Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels.

Autor: Bilverstone TW; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK., Kinsmore NL; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK; NIHR Nottingham Digestive Diseases, (NDCC) Biomedical Research Unit, Nottingham University Hospitals NHS Trust and the University of Nottingham, NG7 2RD, UK., Minton NP; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK; NIHR Nottingham Digestive Diseases, (NDCC) Biomedical Research Unit, Nottingham University Hospitals NHS Trust and the University of Nottingham, NG7 2RD, UK. Electronic address: nigel.minton@nottingham.ac.uk., Kuehne SA; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK; NIHR Nottingham Digestive Diseases, (NDCC) Biomedical Research Unit, Nottingham University Hospitals NHS Trust and the University of Nottingham, NG7 2RD, UK.

Publikováno v: Anaerobe [Anaerobe] 2017 Apr; Vol. 44, pp. 51-54. Date of Electronic Publication: 2017 Jan 17.