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pro vyhledávání: '"Bill Brizzard"'
Autor:
Sarah Trimpin, Bill Brizzard
Publikováno v:
BioTechniques, Vol 46, Iss 6, Pp 409-419 (2009)
The analysis of insoluble proteins represents a major technical challenge for the field of proteomics. For example, membrane proteins are often insoluble in common solvents and represent 20–30% of the proteins encoded by the human genome. Chemical
Externí odkaz:
https://doaj.org/article/1c210a59116843a690ded2e1f1d471a7
Publikováno v:
BioTechniques, Vol 28, Iss 4, Pp 789-793 (2000)
Three FLAG epitopes have been incorporated into the mammalian expression vector pCMV-5 to create a transient expression vector, p3XFLAG-CMV-7. The vector was designed to express FLAG fusion proteins that can be detected at tenfold lower expression le
Externí odkaz:
https://doaj.org/article/70e935f6ddab4cdab9ea1e5418451f53
Autor:
Bill Brizzard
Publikováno v:
BioTechniques, Vol 44, Iss 5, Pp 693-695 (2008)
Epitope tagging is widely used in the characterization of newly discovered proteins. This review presents an overview of how the technique evolved and how it is being used today, with a focus on its use in the study of protein-protein interactions. I
Externí odkaz:
https://doaj.org/article/b48c6852fcc94369a4c3bfc65e25744f
Autor:
Bill Brizzard, Sarah Trimpin
Publikováno v:
BioTechniques. 46(6)
The analysis of insoluble proteins represents a major technical challenge for the field of proteomics. For example, membrane proteins are often insoluble in common solvents and represent 20–30% of the proteins encoded by the human genome. Chemical
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a88bf678739540a699aeadcae591afa
https://pubmed.ncbi.nlm.nih.gov/19480635
https://pubmed.ncbi.nlm.nih.gov/19480635
Publikováno v:
BioTechniques. 28(4)
Three FLAG epitopes have been incorporated into the mammalian expression vector pCMV-5 to create a transient expression vector, p3XFLAG-CMV-7. The vector was designed to express FLAG fusion proteins that can be detected at tenfold lower expression le
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9bba269b7c67fe8a0a35d9800b858b43
https://pubmed.ncbi.nlm.nih.gov/10769759
https://pubmed.ncbi.nlm.nih.gov/10769759