Biophysical Studies of the Induced Dimerization of Human VEGF Receptor 1 Binding Domain by Divalent Metals Competing with VEGF-A.

Autor: Jean-François Gaucher, Marie Reille-Seroussi, Nathalie Gagey-Eilstein, Sylvain Broussy, Pascale Coric, Bili Seijo, Marie-Bernard Lascombe, Benoit Gautier, Wang-Quing Liu, Florent Huguenot, Nicolas Inguimbert, Serge Bouaziz, Michel Vidal, Isabelle Broutin

Publikováno v: PLoS ONE, Vol 11, Iss 12, p e0167755 (2016)

Angiogenesis is tightly regulated through the binding of vascular endothelial growth factors (VEGFs) to their receptors (VEGFRs). In this context, we showed that human VEGFR1 domain 2 crystallizes in the presence of Zn2+, Co2+ or Cu2+ as a dimer that

Externí odkaz: https://doaj.org/article/945d9e856baa4145be209985052c44c6

Transcriptional reprogramming by IL-2 variant generates metabolically active stem-like T cells

Autor: Yaquelin Ortiz-Miranda, Maria Masid, Cristina Jiménez-Luna, Galia Magela Montalvo Bereau, Tania Muller, Nicolas Rayroux, Elisabetta Cribioli, Jesús Corría-Osorio, Helen Carrasco Hope, Romain Vuillefroy de Silly, Bili Seijo, Pierpaolo Ginefra, Kalet León, Nicola Vannini, Ping-Chih Ho, Isaac Crespo, Vassily Hatzimanikatis, Melita Irving, George Coukos

SummaryInterleukin-2 receptor (IL-2R)-mediated intracellular signaling is a key regulator of T-cell fate decisions. While the potent signals generated by IL-2 engagement execute effector differentiation, elevated metabolic activities and rapid cellul

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a54cc544864cfa0ccfa381ec77d49301
https://doi.org/10.1101/2023.05.24.541283

Coexpression and Copurification of RNA-Protein Complexes in Escherichia coli

Autor: Margot, El Khouri, Marjorie, Catala, Bili, Seijo, Johana, Chabal, Frédéric, Dardel, Carine, Tisné, Luc, Ponchon

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2323

For structural, biochemical, or pharmacological studies, it is required to have pure RNA in large quantities. We previously devised a generic approach that allows for efficient in vivo expression of recombinant RNA in Escherichia coli. We have extend

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::33cd207276b33f96fef0bcdeda4c792d
https://pubmed.ncbi.nlm.nih.gov/34086274

Expression and Purification of RNA-Protein Complexes in Escherichia coli

Autor: Margueritte, El Khouri, Marjorie, Catala, Bili, Seijo, Johana, Chabal, Carine, Tisné, Frédéric, Dardel, Luc, Ponchon

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1316

For structural, biochemical or pharmacological studies, it is required to have pure RNA in large quantities. We previously devised a generic approach that allows efficient in vivo expression of recombinant RNA in Escherichia coli. We have extended th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::2f6a2a6c7de21b22ae0e7bbd6814b61d
https://pubmed.ncbi.nlm.nih.gov/25967050

In vivo tmRNA protection by SmpB and pre-ribosome binding conformation in solution

Autor: Luc Ponchon, Frédéric Dardel, Bili Seijo, Jean-Michel Saliou, Patrice Vachette, Ehsan Ranaei-Siadat, Sylvie Nonin-Lecomte, Julie Bernauer, Cécile Mérigoux, Sarah Sanglier-Cianférani

Publikováno v: RNA
RNA, Cold Spring Harbor Laboratory Press, 2014, 20 (10), pp.1607-1620. ⟨10.1261/rna.045674.114⟩
RNA, 2014, 20 (10), pp.1607-1620. ⟨10.1261/rna.045674.114⟩
RNA, Cold Spring Harbor Laboratory Press, 2014, 20 (10), pp.1607-20. 〈10.1261/rna.045674.114〉
RNA, Cold Spring Harbor Laboratory Press, 2014, 20 (10), pp.1607-20. ⟨10.1261/rna.045674.114⟩
Europe PubMed Central

TmRNA is an abundant RNA in bacteria with tRNA and mRNA features. It is specialized in trans-translation, a translation rescuing system. We demonstrate that its partner protein SmpB binds the tRNA-like region (TLD) in vivo and chaperones the fold of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::625aecfb5a6852855764deddb28b7183
https://hal.archives-ouvertes.fr/hal-02344587