Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Bikash K. Bhandari"'
Autor:
Bikash K Bhandari, Chun Shen Lim, Daniela M Remus, Augustine Chen, Craig van Dolleweerd, Paul P Gardner
Publikováno v:
PLoS Computational Biology, Vol 17, Iss 10, p e1009461 (2021)
Recombinant protein production is a key process in generating proteins of interest in the pharmaceutical industry and biomedical research. However, about 50% of recombinant proteins fail to be expressed in a variety of host cells. Here we show that t
Externí odkaz:
https://doaj.org/article/8ffe8742eb264ab78676a7e11bd5d096
Publikováno v:
Bioinformatics
MotivationRecombinant protein production is a widely used technique in the biotechnology and biomedical industries, yet only a quarter of target proteins are soluble and can therefore be purified.ResultsWe have discovered that global structural flexi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69a4d919159f6828589661d77dc44c09
http://europepmc.org/articles/PMC7750957
http://europepmc.org/articles/PMC7750957
Publikováno v:
Nucleic Acids Research
Experiments that are planned using accurate prediction algorithms will mitigate failures in recombinant protein production. We have developed TISIGNER (https://tisigner.com) with the aim of addressing technical challenges to recombinant protein produ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b74c867a5a31c09a608bb8f363d977bc
https://pubmed.ncbi.nlm.nih.gov/33744969
https://pubmed.ncbi.nlm.nih.gov/33744969
MotivationSignal peptides are responsible for protein transport and secretion and are ubiquitous to all forms of life. The annotation of signal peptides is important for understanding protein translocation and toxin secretion and evolution.ResultsHer
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bd13459740403099da9a27873afd4c46
https://doi.org/10.1101/2020.11.30.405613
https://doi.org/10.1101/2020.11.30.405613
Planning experiments using accurate prediction algorithms could mitigate failures in recombinant protein production. We have developed TISIGNER.com with the aim of addressing the technical challenges in recombinant protein production. We offer two we
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5417f5c7e3fcb5ae877ad1ea8e3ac027
https://doi.org/10.1101/2020.07.09.196469
https://doi.org/10.1101/2020.07.09.196469
Autor:
Paul P. Gardner, Augustine Chen, Craig J. van Dolleweerd, Chun Shen Lim, Daniela M. Remus, Bikash K. Bhandari
Publikováno v:
PLoS Computational Biology, Vol 17, Iss 10, p e1009461 (2021)
PLoS Computational Biology
PLoS Computational Biology
Recombinant protein production is a key process in generating proteins of interest in the pharmaceutical industry and biomedical research. However, about 50% of recombinant proteins fail to be expressed in a variety of host cells. Here we show that t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b4e4f26b40648f03e797c5a7b104256
https://doi.org/10.1371/journal.pcbi.1009461
https://doi.org/10.1371/journal.pcbi.1009461
Autor:
Daniela M. Remus, Paul P. Gardner, Bikash K. Bhandari, Chun Shen Lim, Craig J. van Dolleweerd, Augustine Chen
Recombinant protein production is a widely used technique, yet half of these experiments fail at the expression phase. Failures are expected for 9difficult-to-express9 proteins, but for others, codon bias and mRNA folding have been proposed explanati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::371b0c2de8ea1a4110dacb1870dd1073
https://doi.org/10.1101/726752
https://doi.org/10.1101/726752