Výsledky vyhledávání - "Bijoy K. Mohanty"

Inactivation of RNase P in Escherichia coli significantly changes post‐transcriptional RNA metabolism

Autor: Sidney R. Kushner, Bijoy K. Mohanty

Publikováno v: Mol Microbiol

Ribonuclease P (RNase P), which is required for the 5'-end maturation of tRNAs in every organism, has been shown to play a limited role in other aspects of RNA metabolism in Escherichia coli. Using RNA-sequencing (RNA-seq), we demonstrate that RNase

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c1742a6638c6f90712a142d33338fd92
https://doi.org/10.1111/mmi.14808

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Regulation of mRNA decay in E. coli

Autor: Sidney R. Kushner, Bijoy K. Mohanty

Publikováno v: Critical Reviews in Biochemistry and Molecular Biology. 57:48-72

Detailed studies of the Gram-negative model bacterium, Escherichia coli, have demonstrated that post-transcriptional events exert important and possibly greater control over gene regulation than tr...

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2d78c754fc358c793de14b40dba712e1
https://doi.org/10.1080/10409238.2021.1968784

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Processing of the alaW alaX operon encoding the Ala2 tRNAs in Escherichia coli requires both RNase E and RNase P

Autor: Bijoy K, Mohanty, Sidney R, Kushner

Publikováno v: Molecular microbiologyREFERENCES. 118(6)

The alaW alaX operon encodes the Ala2 tRNAs, one of the two alanine tRNA isotypes in Escherichia coli. Our previous RNA-seq study showed that alaW alaX dicistronic RNA levels increased significantly in the absence of both RNase P and poly(A) polymera

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c5d71e742630633272263a0543240745
https://pubmed.ncbi.nlm.nih.gov/36268779

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Maturation of the E. coli Glu2, Ile1, and Ala1B tRNAs utilizes a complex processing pathway

Autor: Bijoy K. Mohanty, Keri Nichols, Sidney R. Kushner

Publikováno v: Molecular microbiologyREFERENCES. 118(1-2)

Despite significant progress in understanding the diversity of tRNA processing pathways in Escherichia coli, the mechanism for the maturation of tRNAs encoded within the rRNA operons has not received much attention. Here, we show that the Glu2, Ile1,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6074a719d887cd2865dd91b094b486be
https://pubmed.ncbi.nlm.nih.gov/35652235

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The C nucleotide at the mature 5' end of the Escherichia coli proline tRNAs is required for the RNase E cleavage specificity at the 3' terminus as well as functionality

Autor: Bijoy K Mohanty, Valerie Maples, Sidney R Kushner

Publikováno v: Nucleic acids research. 50(3)

Proline tRNA 3′-maturation in Escherichia coli occurs through a one-step RNase E endonucleolytic cleavage immediately after the CCA determinant. This processing pathway is distinct from the 3′-end maturation of the other tRNAs by avoiding the wid

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f349fc0024e3031af379f7e8ccef404
https://pubmed.ncbi.nlm.nih.gov/35061897

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Regulation of mRNA decay in

Autor: Bijoy K, Mohanty, Sidney R, Kushner

Publikováno v: Crit Rev Biochem Mol Biol

Detailed studies of the Gram-negative model bacterium, Escherichia coli, have demonstrated that post-transcriptional events exert important and possibly greater control over gene regulation than transcription initiation or effective translation. Thus

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::68938a823882098e4faee3c442c27c2b
https://pubmed.ncbi.nlm.nih.gov/34547957

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Generation of pre-tRNAs from polycistronic operons is the essential function of RNase P in Escherichia coli

Autor: Sidney R. Kushner, Ankit Agrawal, Bijoy K. Mohanty

Publikováno v: Nucleic Acids Research

Ribonuclease P (RNase P) is essential for the 5′-end maturation of tRNAs in all kingdoms of life. In Escherichia coli, temperature sensitive mutations in either its protein (rnpA49) and or RNA (rnpB709) subunits lead to inviability at nonpermissive

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0b56371706068461cf7e389dfb678a7
https://doi.org/10.1093/nar/gkz1188

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Analysis of post-transcriptional RNA metabolism in prokaryotes

Autor: Bijoy K. Mohanty, Sidney R. Kushner

Publikováno v: Methods. 155:124-130

Post-transcriptional RNA metabolic pathways play important roles in permitting prokaryotes to operate under a variety of environmental conditions. Although significant progress has been made during the last decade in deciphering RNA processing pathwa

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https://doi.org/10.1016/j.ymeth.2018.11.006

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Regulation of mRNA Decay in Bacteria

Autor: Bijoy K. Mohanty, Sidney R. Kushner

Publikováno v: Annual Review of Microbiology. 70:25-44

Gram-negative and gram-positive bacteria use a variety of enzymatic pathways to degrade mRNAs. Although several recent reviews have outlined these pathways, much less attention has been paid to the regulation of mRNA decay. The functional half-life o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f07518de179d8df23543479bc7597e02
https://doi.org/10.1146/annurev-micro-091014-104515

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