Compressed sensing for electron cryotomography and high-resolution subtomogram averaging of biological specimens

Autor: Böhning, J, Bharat, TAM, Collins, SM

Publikováno v: Structure. 30:408-417.e4

Cryoelectron tomography (cryo-ET) and subtomogram averaging (STA) allow direct visualization and structural studies of biological macromolecules in their native cellular environment, in situ. Often, low signal-to-noise ratios in tomograms, low partic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b55c2d8eee1041b5d3b73188ac63e6b1
https://doi.org/10.1016/j.str.2021.12.010

Rod-shaped tricalbins contribute to PM asymmetry at curved ER-PM contact sites

Autor: Bharat Tam., Patrick C. Hoffmann, Michael R. Wozny, Wanda Kukulski, Elizabeth A. Miller

Lipid flow between cellular organelles occurs via membrane contact sites that form dynamic conduits. Extended-synaptotagmins, known as tricalbins in yeast, mediate lipid transfer between the endoplasmic reticulum (ER) and plasma membrane (PM). How th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d5a6e04a86bcde2fedd47200235b55a

Resolving macromolecular structures from electron cryo-tomography data using sub-tomogram averaging in RELION

Autor: Bharat, TAM, Scheres, SHW

Electron cryo-tomography (cryo-ET) is a technique that is used to produce three-dimensional pictures (tomograms) of complex objects like asymmetric viruses, cellular organelles or whole cells from a series of tilted electron cryo-microscopy (cryo-EM)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::37417685fe171c9241c634c036ee0c74
https://europepmc.org/articles/PMC5215819/

An image processing pipeline for electron cryo-tomography in RELION-5.

Autor: Burt A; MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK.; Department of Structural Biology, Genentech, South San Francisco, CA, USA., Toader B; MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK., Warshamanage R; CCP-EM, Scientific Computing Department, UKRI Science and Technology Facilities Council, Harwell Campus, Didcot, UK.; Department of Psychiatry, University of Pittsburgh, Pittsburgh, PA, USA., von Kügelgen A; MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK., Pyle E; Institute of Structural and Molecular Biology, Birkbeck College, London, UK.; The Francis Crick Institute, London, UK., Zivanov J; MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK., Kimanius D; MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK., Bharat TAM; MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK., Scheres SHW; MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK.

Publikováno v: FEBS open bio [FEBS Open Bio] 2024 Nov; Vol. 14 (11), pp. 1788-1804. Date of Electronic Publication: 2024 Aug 15.

The parasitic lifestyle of an archaeal symbiont.

Autor: Hamm JN; School of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW, 2052, Australia. josh.n.hamm@gmail.com.; Department of Marine Microbiology and Biogeochemistry, Royal Netherlands Institute for Sea Research, Den Hoorn, The Netherlands, 1797 SZ. josh.n.hamm@gmail.com., Liao Y; Australian Institute for Microbiology and Infection, University of Technology Sydney, Ultimo, NSW, 2007, Australia., von Kügelgen A; Structural Studies Division, MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK.; Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, UK., Dombrowski N; Department of Marine Microbiology and Biogeochemistry, Royal Netherlands Institute for Sea Research, Den Hoorn, The Netherlands, 1797 SZ., Landers E; School of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW, 2052, Australia., Brownlee C; Biological Resources Imaging Laboratory, Mark Wainwright Analytical Centre, University of New South Wales, Sydney, NSW, 2052, Australia.; Fluorescence Analysis Facility, Molecular Horizons, University of Wollongong, Keiraville, NSW, 2522, Australia., Johansson EMV; Biological Resources Imaging Laboratory, Mark Wainwright Analytical Centre, University of New South Wales, Sydney, NSW, 2052, Australia., Whan RM; Katharina Gaus Light Microscopy Facility, Mark Wainwright Analytical Centre, The University of New South Wales, Sydney, NSW, 2052, Australia., Baker MAB; School of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW, 2052, Australia., Baum B; Cell Biology Division, MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK., Bharat TAM; Structural Studies Division, MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK.; Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, UK., Duggin IG; Australian Institute for Microbiology and Infection, University of Technology Sydney, Ultimo, NSW, 2007, Australia., Spang A; Department of Marine Microbiology and Biogeochemistry, Royal Netherlands Institute for Sea Research, Den Hoorn, The Netherlands, 1797 SZ.; Department of Evolutionary & Population Biology, Institute for Biodiversity and Ecosystem Dynamics (IBED), University of Amsterdam, Amsterdam, The Netherlands., Cavicchioli R; School of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW, 2052, Australia.

Publikováno v: Nature communications [Nat Commun] 2024 Jul 31; Vol. 15 (1), pp. 6449. Date of Electronic Publication: 2024 Jul 31.