An image processing pipeline for electron cryo-tomography in RELION-5.

Autor: Burt A; MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK.; Department of Structural Biology, Genentech, South San Francisco, CA, USA., Toader B; MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK., Warshamanage R; CCP-EM, Scientific Computing Department, UKRI Science and Technology Facilities Council, Harwell Campus, Didcot, UK.; Department of Psychiatry, University of Pittsburgh, Pittsburgh, PA, USA., von Kügelgen A; MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK., Pyle E; Institute of Structural and Molecular Biology, Birkbeck College, London, UK.; The Francis Crick Institute, London, UK., Zivanov J; MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK., Kimanius D; MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK., Bharat TAM; MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK., Scheres SHW; MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK.

Publikováno v: FEBS open bio [FEBS Open Bio] 2024 Nov; Vol. 14 (11), pp. 1788-1804. Date of Electronic Publication: 2024 Aug 15.

The parasitic lifestyle of an archaeal symbiont.

Autor: Hamm JN; School of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW, 2052, Australia. josh.n.hamm@gmail.com.; Department of Marine Microbiology and Biogeochemistry, Royal Netherlands Institute for Sea Research, Den Hoorn, The Netherlands, 1797 SZ. josh.n.hamm@gmail.com., Liao Y; Australian Institute for Microbiology and Infection, University of Technology Sydney, Ultimo, NSW, 2007, Australia., von Kügelgen A; Structural Studies Division, MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK.; Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, UK., Dombrowski N; Department of Marine Microbiology and Biogeochemistry, Royal Netherlands Institute for Sea Research, Den Hoorn, The Netherlands, 1797 SZ., Landers E; School of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW, 2052, Australia., Brownlee C; Biological Resources Imaging Laboratory, Mark Wainwright Analytical Centre, University of New South Wales, Sydney, NSW, 2052, Australia.; Fluorescence Analysis Facility, Molecular Horizons, University of Wollongong, Keiraville, NSW, 2522, Australia., Johansson EMV; Biological Resources Imaging Laboratory, Mark Wainwright Analytical Centre, University of New South Wales, Sydney, NSW, 2052, Australia., Whan RM; Katharina Gaus Light Microscopy Facility, Mark Wainwright Analytical Centre, The University of New South Wales, Sydney, NSW, 2052, Australia., Baker MAB; School of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW, 2052, Australia., Baum B; Cell Biology Division, MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK., Bharat TAM; Structural Studies Division, MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK.; Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, UK., Duggin IG; Australian Institute for Microbiology and Infection, University of Technology Sydney, Ultimo, NSW, 2007, Australia., Spang A; Department of Marine Microbiology and Biogeochemistry, Royal Netherlands Institute for Sea Research, Den Hoorn, The Netherlands, 1797 SZ.; Department of Evolutionary & Population Biology, Institute for Biodiversity and Ecosystem Dynamics (IBED), University of Amsterdam, Amsterdam, The Netherlands., Cavicchioli R; School of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, NSW, 2052, Australia.

Publikováno v: Nature communications [Nat Commun] 2024 Jul 31; Vol. 15 (1), pp. 6449. Date of Electronic Publication: 2024 Jul 31.

Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus.

Autor: Herdman M; Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, UK., Isbilir B; Structural Studies Division, MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK., von Kügelgen A; Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, UK.; Structural Studies Division, MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK., Schulze U; Cell Biology Division, MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK., Wainman A; Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, UK., Bharat TAM; Structural Studies Division, MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK. tbharat@mrc-lmb.cam.ac.uk.

Publikováno v: Nature communications [Nat Commun] 2024 Apr 18; Vol. 15 (1), pp. 3355. Date of Electronic Publication: 2024 Apr 18.

Cryo-EM of soft-landed β-galactosidase: Gas-phase and native structures are remarkably similar.

Autor: Esser TK; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.; Kavli Institute for NanoScience Discovery, Dorothy Crowfoot Hodgkin Building, Oxford OX1 3QU, UK.; Thermo Fisher Scientific, 1 Boundary Park, Hemel Hempstead, Hertfordshire HP2 7GE, UK., Böhning J; Structural Studies Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK., Önür A; Department of Chemistry, University of Konstanz, Konstanz 78457, Germany., Chinthapalli DK; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.; Kavli Institute for NanoScience Discovery, Dorothy Crowfoot Hodgkin Building, Oxford OX1 3QU, UK., Eriksson L; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.; Kavli Institute for NanoScience Discovery, Dorothy Crowfoot Hodgkin Building, Oxford OX1 3QU, UK., Grabarics M; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.; Kavli Institute for NanoScience Discovery, Dorothy Crowfoot Hodgkin Building, Oxford OX1 3QU, UK., Fremdling P; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Konijnenberg A; Thermo Fisher Scientific, De Schakel 2, 5651GH Eindhoven, Netherlands., Makarov A; Thermo Fisher Scientific, Bremen 28199, Germany.; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, Netherlands., Botman A; Thermo Fisher Scientific, 5350 NE Dawson Creek Drive, Hillsboro, OR 97124, USA., Peter C; Department of Chemistry, University of Konstanz, Konstanz 78457, Germany., Benesch JLP; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.; Kavli Institute for NanoScience Discovery, Dorothy Crowfoot Hodgkin Building, Oxford OX1 3QU, UK., Robinson CV; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.; Kavli Institute for NanoScience Discovery, Dorothy Crowfoot Hodgkin Building, Oxford OX1 3QU, UK., Gault J; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Baker L; Kavli Institute for NanoScience Discovery, Dorothy Crowfoot Hodgkin Building, Oxford OX1 3QU, UK.; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK., Bharat TAM; Structural Studies Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK., Rauschenbach S; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.; Kavli Institute for NanoScience Discovery, Dorothy Crowfoot Hodgkin Building, Oxford OX1 3QU, UK.

Publikováno v: Science advances [Sci Adv] 2024 Feb 16; Vol. 10 (7), pp. eadl4628. Date of Electronic Publication: 2024 Feb 14.

The cell envelope architecture of Deinococcus: HPI forms the S-layer and SlpA tethers the outer membrane to peptidoglycan.

Autor: Bharat TAM; Structural Studies Division, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom., Tocheva EI; Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada., Alva V; Department of Protein Evolution, Max Planck Institute for Biology Tübingen, Tübingen 72076, Germany.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2023 Dec 19; Vol. 120 (51), pp. e2305338120. Date of Electronic Publication: 2023 Dec 12.