Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Beverley A. Yard"'
Autor:
Marine C.C. Raman, Beverley A. Yard, James H. Naismith, Lester G. Carter, Jonathan Lowther, Kenneth A. Johnson, Dominic J. Campopiano
Publikováno v:
Journal of Biological Chemistry. 284:17328-17339
Sphingolipid biosynthesis begins with the condensation of l-serine and palmitoyl-CoA catalyzed by the PLP-dependent enzyme serine palmitoyltransferase (SPT). Mutations in human SPT cause hereditary sensory autonomic neuropathy type 1, a disease chara
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::557bd1c95b680a4a275a57a62559e2b2
https://doi.org/10.1074/jbc.m109.008680
https://doi.org/10.1074/jbc.m109.008680
Autor:
John P. Iredale, Manfred Auer, Xiaozhong Zheng, Damian J. Mole, Margareth Binnie, Scott P. Webster, Kris Wilson, Natalie Z.M. Homer, Beverley A. Yard
Publikováno v:
Wilson, K, Mole, D J, Binnie, M, Homer, N Z M, Zheng, X, Yard, B A, Iredale, J P, Auer, M & Webster, S P 2014, ' Bacterial expression of human kynurenine 3-monooxygenase : Solubility, activity, purification ', Protein Expression and Purification, vol. 95, pp. 96-103 . https://doi.org/10.1016/j.pep.2013.11.015
Protein Expression and Purification
Protein Expression and Purification
Highlights • This is the first report of soluble and active bacterially expressed human KMO protein. • Partial purification of the enzyme was achieved and the two protein co-elutants identified. • Steady state kinetic parameters were comparable
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee2a009729b9b38abbb39a12fde90afc
https://hdl.handle.net/20.500.11820/92c8c248-fc66-4a3d-a187-8ee4da5a717a
https://hdl.handle.net/20.500.11820/92c8c248-fc66-4a3d-a187-8ee4da5a717a
Autor:
Bill Leavens, Jonathan P. Hutchinson, Carl Haslam, Damian J. Mole, John Liddle, Scott P. Webster, Beverley A. Yard, Argyrides Argyrou, Erica Christodoulou, Margaret Binnie, Paul Hissey, P. Hardwicke, Kris Wilson, Denise M. Lowe, Tony W. Dean, Iain Uings, Michelle Gee
Publikováno v:
Journal of biomolecular screening. 19(4)
Kynurenine 3-monooxygenase (KMO) is a therapeutically important target on the eukaryotic tryptophan catabolic pathway, where it converts L-kynurenine (Kyn) to 3-hydroxykynurenine (3-HK). We have cloned and expressed the human form of this membrane pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cfd2b2fee2bad3fa1390a14c9721233e
https://pubmed.ncbi.nlm.nih.gov/24381207
https://pubmed.ncbi.nlm.nih.gov/24381207
Autor:
Dominic J. Campopiano, Britta Ramakers, David Clarke, Venugopal T. Bhat, Marine C.C. Raman, Kenneth A. Johnson, Lester G. Carter, Beverley A. Yard, Stephen A. McMahon, James H. Naismith, Jonathan Lowther
Publikováno v:
Lowther, J, Yard, B A, Johnson, K A, Carter, L G, Bhat, V T, Raman, M C C, Clarke, D J, Ramakers, B, McMahon, S A, Naismith, J H & Campopiano, D J 2010, ' Inhibition of the PLP-dependent enzyme serine palmitoyltransferase by cycloserine: evidence for a novel decarboxylative mechanism of inactivation ', Molecular BioSystems, vol. 6, no. 9, pp. 1682-1693 . https://doi.org/10.1039/c003743e
Cycloserine (CS, 4-amino-3-isoxazolidone) is a cyclic amino acid mimic that is known to inhibit many essential pyridoxal 5'-phosphate (PLP)-dependent enzymes. Two CS enantiomers are known; D-cycloserine (DCS, also known as Seromycin) is a natural pro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c987a737c9f607c33e914a30105e6684
https://europepmc.org/articles/PMC3670083/
https://europepmc.org/articles/PMC3670083/
Autor:
Lester G. Carter, Muse Oke, Kenneth A. Johnson, Ian M. Overton, Dominic J. Campopiano, Daphné Puech, Geoffrey J. Barton, Beverley A. Yard, James H. Naismith, Stephen A. McMahon, Mark Dorward, Huanting Liu
Publikováno v:
Journal of molecular biology. 370(5)
Sphingolipid biosynthesis commences with the condensation of L-serine and palmitoyl-CoA to produce 3-ketodihydrosphingosine (KDS). This reaction is catalysed by the PLP-dependent enzyme serine palmitoyltransferase (SPT; EC 2.3.1.50), which is a membr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50fc390e8c93997ba0788c758d1c5d0a
https://pubmed.ncbi.nlm.nih.gov/17559874
https://pubmed.ncbi.nlm.nih.gov/17559874