Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Bert Top"'
Autor:
Magdi H. Yacoub, Nicholas R. Banner, Mahboob A. Khan, Bert Top, Ghada W. Mikhail, Nicole M. M. Tacken, Nienke Oldenburg, Jaap M. Middeldorp, Kitty M. C. Lam, Anne Mckie, Andrew Wright
Publikováno v:
Transplantation. 70:1209-1215
Background. Human cytomegalovirus (CMV) infection is a major cause of morbidity in transplant patients. Early diagnosis and treatment have been shown to improve outcome. We evaluated the suitability of CMV immediate early, early, and late gene expres
Autor:
Bert Top, Sjoerd Rodenhuis, Wolter J. Mooi, Caro Lambrechts, R. A. Maas, Laura J. vanʼt Veer, Hans Peterse, Majella S. de Lange
Publikováno v:
Diagnostic Molecular Pathology. 6:353-360
A method based on the reverse transcriptase-polymerase chain reaction (RT-PCR) was developed that allows the determination of relative mRNA expression levels in fine-needle aspirates from human tumors. The method was developed for the c-erbB-2 gene,
Autor:
Siegina G. Klaver, Lucie Boerrigter, Sjoerd Visser, Sjoerd Rodenhuis, Peter Wisman, Bert Top, Hans R. J. Elbers, Wolter J. Mooi
Publikováno v:
International Journal of Cancer. 64:83-91
A series of 54 resected primary non-small-cell lung carcinomas was analyzed for p53 gene mutations and for p53 protein accumulation and the findings were correlated with clinical parameters. Mutations in exons 5 through 8 of the p53 gene were identif
Autor:
Richard J. Sinke, Luc Beckers, Peter Huijser, Bert Top, Monique M.P. Hermans, Wolfgang Hennig
Publikováno v:
Chromosoma, 100(1), 48-55
Microdissection of the lampbrush loops "threads" and "pseudonucleolus" of Y chromosomes from primary spermatocytes of Drosophila hydei and subsequent microcloning of the DNA yielded several recombinant DNA clones which cross-hybridized in screening t
Autor:
M. H. L. Christiaans, Marinus J. Blok, Valère J. Goossens, J.P. van Hooff, Cathrien A. Bruggeman, Bert Top, Jaap M. Middeldorp
Publikováno v:
Transplant International, 11 Suppl 1, S107-9. Wiley-Blackwell
Blok, M J, Christiaans, M H, Goossens, V J, van Hooff, J P, Top, B, Middeldorp, J M & Bruggeman, C A 1998, ' Evaluation of a new method for early detection of active cytomegalovirus infections. A study in kidney transplant recipients ', Transplant International, vol. 11 Suppl 1, pp. S107-9 .
Blok, M J, Christiaans, M H, Goossens, V J, van Hooff, J P, Top, B, Middeldorp, J M & Bruggeman, C A 1998, ' Evaluation of a new method for early detection of active cytomegalovirus infections. A study in kidney transplant recipients ', Transplant International, vol. 11 Suppl 1, pp. S107-9 .
Early detection of active cytomegalovirus (CMV) infection after organ transplantation is necessary to start effective antiviral treatment. In the present study, blood specimens of kidney transplant recipients (n = 38) were monitored for the expressio
Publikováno v:
International journal of cancer. Journal international du cancer, 57(3), 362-364. Wiley-Liss Inc.
We have examined 17 adenocarcinomas and 2 mixed tumors of the salivary glands for mutational activation of the oncogenes H-ras, K-ras and N-ras. The presence of mutations was determined by in vitro amplification of gene fragments spanning codons 12,
Autor:
Annemarie Borst, R Schukkink, Nicole M. M. Tacken, Adrienne T.A. Box, Ad C. Fluit, Bob van Gemen, Jan Verhoef, M. N. Widjojoatmodjo, Bert Top
Publikováno v:
Journal of microbiological methods. 38(1-2)
Nucleic Acid Sequence Based Amplification (iNASBA), an isothermal amplification technique for nucleic acids, was evaluated for the identification of medically important Candida species using primers selected from 18S rRNA sequences conserved in fungi
Publikováno v:
Nucleic acids research. 26(9)
NASBA is an isothermal nucleic acid amplification reaction that amplifies mRNA in a dsDNA background. Although similar to the sensitive reverse transcription/polymerase chain reaction (RT-PCR) in mRNA detection, NASBA is not prone to give false posit
Autor:
Valère J. Goossens, Bert Top, Maarten H. L. Christiaans, Sabina J. V. Vanherle, Nicole M. M. Tacken, Cathrien A. Bruggeman, Jaap M. Middeldorp, Johannes P. van Hooff, Marinus J. Blok
Publikováno v:
Journal of Clinical Microbiology, 36(5), 1341-6. American Society for Microbiology
Journal of Clinical Microbiology, 36(5), 1341-1346. American Society for Microbiology
Europe PubMed Central
Blok, M J, Goossens, V J, Vanherle, S J, Top, B, Tacken, N, Middeldorp, J M, Christiaans, M H, van Hooff, J P & Bruggeman, C A 1998, ' Diagnostic value of monitoring human cytomegalovirus late pp67 mRNA expression in renal-allograft recipients by nucleic acid sequence-based amplification ', Journal of Clinical Microbiology, vol. 36, no. 5, pp. 1341-6 .
Journal of Clinical Microbiology, 36(5), 1341-1346. American Society for Microbiology
Europe PubMed Central
Blok, M J, Goossens, V J, Vanherle, S J, Top, B, Tacken, N, Middeldorp, J M, Christiaans, M H, van Hooff, J P & Bruggeman, C A 1998, ' Diagnostic value of monitoring human cytomegalovirus late pp67 mRNA expression in renal-allograft recipients by nucleic acid sequence-based amplification ', Journal of Clinical Microbiology, vol. 36, no. 5, pp. 1341-6 .
Department of Medical Microbiology, University Hospital Maastricht, The Netherlands. mbl@lmib.azm.nlThe diagnostic value of monitoring human cytomegalovirus (HCMV) late pp67 mRNA expression by nucleic acid sequence-based amplification (NASBA) after r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::337fe4055dda49241e2e94e9a26057c3
https://research.vumc.nl/en/publications/d92ac01c-5b6b-45f5-ac7c-d7edb169b939
https://research.vumc.nl/en/publications/d92ac01c-5b6b-45f5-ac7c-d7edb169b939
Publikováno v:
Cancer letters. 97(1)
To find early and sensitive indicators of treatment response in breast cancer, we studied the mRNA levels of proliferation-related genes during growth arrest of the human breast cancer cell lines T47D and MCF7. A sensitive reverse transcriptase-PCR (