Zobrazeno 1 - 10
of 282
pro vyhledávání: '"Bernhard Hauer"'
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-10 (2024)
Abstract Terpene synthesis stands at the forefront of modern synthetic chemistry and represents the state-of-the-art in the chemist’s toolbox. Notwithstanding, these endeavors are inherently tied to the current availability of natural cyclic buildi
Externí odkaz:
https://doaj.org/article/3636d2985a7b4fc0afb096bf98cadb5e
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-9 (2022)
Squalene-hopene cyclase are membrane-bound enzymes used to produce bioactive compounds at industrial scale. Here, the authors show squalene-hopene cyclase spheroplasts, obtained by removing the outer cell membrane, having a higher catalytic activity
Externí odkaz:
https://doaj.org/article/afa26c373bbd4cabb2a772c1810626aa
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Active-site loops are important for catalytic properties of enzymes, but challenging to engineer due to their high flexibility and diversity. Here, the authors identify and engineer hot-spots in the loops of cumene dioxygenase, obtain variants with c
Externí odkaz:
https://doaj.org/article/e4cdf7460e154bfdbc8afe31e3043963
Publikováno v:
MethodsX, Vol 6, Iss , Pp 2484-2491 (2019)
Over the past years, neonicotinoids such as thiacloprid and flupyradifurone have gained considerable scientific and public interest. These molecules used as active compounds in pesticides are known due to cause drastic negative long-time effects on p
Externí odkaz:
https://doaj.org/article/228a93237749486a8541465556937258
Autor:
Sandra Engelskirchen, Stefan Wellert, Olaf Holderer, Henrich Frielinghaus, Michaela Laupheimer, Sven Richter, Bettina Nestl, Bernd Nebel, Bernhard Hauer
Publikováno v:
Frontiers in Chemistry, Vol 8 (2021)
Lipase-catalyzed reactions offer many advantages among which a high degree of selectivity combined with the possibility to convert even non-natural substrates are of particular interest. A major drawback in the applicability of lipases in the convers
Externí odkaz:
https://doaj.org/article/c54d50fbd52947128fff02c91c948849
Publikováno v:
MethodsX, Vol 8, Iss , Pp 101285- (2021)
Phosphorous-NMR is scarcely employed to evaluate enzyme kinetics of kinase driven monophosphorylations, despite of being a powerful and reliable tool to undoubtedly detect the actual phosphoryl transfer to the targeted substrate. Another advantage is
Externí odkaz:
https://doaj.org/article/69c213b94788424082ace9360a745db1
Publikováno v:
MethodsX, Vol 8, Iss , Pp 101323- (2021)
Rieske non-heme iron dioxygenases are a class of intriguing enzymes covering a broad reaction and substrate spectrum and have been studied extensively in the last decades. In nature, these biocatalysts are essential for the production of cis-dihydrox
Externí odkaz:
https://doaj.org/article/aa60603e263e429c9bef15abc8d44f33
Publikováno v:
MethodsX, Vol 8, Iss , Pp 101455- (2021)
In an attempt to establish a biosynthetic route towards isobutene, we faced the problem that the first intermediate isobutyl-monophosphate was not commercially available. In order to overcome this limitation we searched in the literature for protocol
Externí odkaz:
https://doaj.org/article/475438de6af3410f99941d606ee63a85
Publikováno v:
MethodsX, Vol 7, Iss , Pp 101143- (2020)
cis-Dihydrodiendiols are valuable compounds, finding multiple application as chiral synthons in organic chemistry. The biotechnological route for the generation of cis-dihydrodiendiols involves the dihydroxylation of aromatic compounds, catalyzed by
Externí odkaz:
https://doaj.org/article/da6e96710b924158a5220f0e0fbe2a04
Publikováno v:
Catalysts, Vol 9, Iss 3, p 252 (2019)
A novel approach for the synthesis of vanillin employing a three-step two-enzymatic cascade sequence is reported. Cytochrome P450 monooxygenases are known to catalyse the selective hydroxylation of aromatic compounds, which is one of the most challen
Externí odkaz:
https://doaj.org/article/11a0baa8a0bd4269aa0551f05077cc84