A modular platform for automated cryo-FIB workflows

Autor: Sven Klumpe, Herman KH Fung, Sara K Goetz, Ievgeniia Zagoriy, Bernhard Hampoelz, Xiaojie Zhang, Philipp S Erdmann, Janina Baumbach, Christoph W Müller, Martin Beck, Jürgen M Plitzko, Julia Mahamid

Publikováno v: eLife, Vol 10 (2021)

Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB) has matured into a preparation method widely used for cellular cryo-electron tomography. Due to the limited ablation rates of low Ga+ ion beam currents required to

Externí odkaz: https://doaj.org/article/dac8598bb5fe4b05b4258e93085e26ee

A modular platform for automated cryo-FIB workflows

Autor: Sara K Goetz, Herman KH Fung, Sven Klumpe, Ievgeniia Zagoriy, Bernhard Hampoelz, Xiaojie Zhang, Philipp S Erdmann, Janina Baumbach, Christoph W Müller, Martin Beck, Jürgen M Plitzko, Julia Mahamid

Publikováno v: eLife
eLife, Vol 10 (2021)
elife

Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB) has matured into a preparation method widely used for cellular cryo-electron tomography. Due to the limited ablation rates of low Ga+ ion beam currents required to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::957649178edea395e1b22cbddd671d81
https://pubmed.ncbi.nlm.nih.gov/34951584

Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis

Autor: Andre Schwarz, Bernhard Hampoelz, Helena Bragulat-Teixidor, Imre Gaspar, Anne Ephrussi, Christian Tischer, Yannick Schwab, Paolo Ronchi, Martin Beck

Publikováno v: Cell

Summary The molecular events that direct nuclear pore complex (NPC) assembly toward nuclear envelopes have been conceptualized in two pathways that occur during mitosis or interphase, respectively. In gametes and embryonic cells, NPCs also occur with

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17101874b1c5d843c29d11864a3d50d3

Pre-assembled Nuclear Pores Insert into the Nuclear Envelope during Early Development

Autor: Bernhard Hampoelz, Khanh Huy Bui, Thomas Lecuit, Yannick Schwab, Aleksandar Necakov, Amparo Andres-Pons, Paolo Ronchi, Martin Beck, Nicole L. Schieber, Jean Philippe, Rachel Santarella-Mellwig, Marie-Therese Mackmull, Pedro Machado

Publikováno v: Cell
Cell, Elsevier, 2016, 166 (3), pp.664-78. ⟨10.1016/j.cell.2016.06.015.Epub2016Jul7.⟩
Cell, 2016, 166 (3), pp.664-78. ⟨10.1016/j.cell.2016.06.015.Epub2016Jul7⟩

Summary Nuclear pore complexes (NPCs) span the nuclear envelope (NE) and mediate nucleocytoplasmic transport. In metazoan oocytes and early embryos, NPCs reside not only within the NE, but also at some endoplasmic reticulum (ER) membrane sheets, term

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21cb0c71a3fae37e23b197ef39d7b952
https://hal.archives-ouvertes.fr/hal-01445583

Heterotrimeric G Proteins

Autor: Juergen A. Knoblich, Bernhard Hampoelz

Publikováno v: Cell. 119:453-456

Heterotrimeric G proteins are well known for their function in signal transduction downstream of seven transmembrane receptors. More recently, however, genetic analysis in C. elegans and in Drosophila has revealed a second, essential function of thes

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::564f0f9e1215dd7b9fff3cb21ddd073f
https://doi.org/10.1016/j.cell.2004.10.025