Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Bernd Zetsche"'
Autor:
Jonathan Strecker, Sara Jones, Balwina Koopal, Jonathan Schmid-Burgk, Bernd Zetsche, Linyi Gao, Kira S. Makarova, Eugene V. Koonin, Feng Zhang
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-8 (2019)
The Cas12b family of CRISPR nucleases has been underutilized in mammalian cells due to the high temperature requirement of known members. Here the authors engineer BhCas12b to overcome this limitation for robust and specific genome editing applicatio
Externí odkaz:
https://doaj.org/article/e6ea12311957487294bb0381d2fecb20
Autor:
Winston X. Yan, Reza Mirzazadeh, Silvano Garnerone, David Scott, Martin W. Schneider, Tomasz Kallas, Joaquin Custodio, Erik Wernersson, Yinqing Li, Linyi Gao, Yana Federova, Bernd Zetsche, Feng Zhang, Magda Bienko, Nicola Crosetto
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-9 (2017)
Double-strand breaks are a major DNA lesion that can occur by endogenous and exogenous processes. Here the authors present BLISS—Breaks LabellingIn Situand Sequencing—to map breaks across the genome.
Externí odkaz:
https://doaj.org/article/09a5cb9f162d419e8462fcfa6062aa33
Autor:
Takashi Yamano, Hiroshi Nishimasu, Bernd Zetsche, Hisato Hirano, Ian M. Slaymaker, Yinqing Li, Iana Fedorova, Takanori Nakane, Kira S. Makarova, Eugene V. Koonin, Ryuichiro Ishitani, Feng Zhang, Osamu Nureki
Publikováno v:
Cell. 165:949-962
Cpf1 is an RNA-guided endonuclease of a type V CRISPR-Cas system that has been recently harnessed for genome editing. Here, we report the crystal structure of Acidaminococcus sp. Cpf1 (AsCpf1) in complex with the guide RNA and its target DNA, at 2.8
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ae1a6f271e6c59a3d2596a220de4cf1
https://doi.org/10.1016/j.cell.2016.04.003
https://doi.org/10.1016/j.cell.2016.04.003
Autor:
Eugene V. Koonin, Jonathan L. Schmid-Burgk, Kira S. Makarova, Jonathan Strecker, Feng Zhang, Balwina Koopal, Bernd Zetsche, Linyi Gao, Sara R. Jones
Publikováno v:
Nature Communications
Nature Communications, Vol 10, Iss 1, Pp 1-8 (2019)
Nature Communications, Vol 10, Iss 1, Pp 1-8 (2019)
The type-V CRISPR effector Cas12b (formerly known as C2c1) has been challenging to develop for genome editing in human cells, at least in part due to the high temperature requirement of the characterized family members. Here we explore the diversity
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ccf39cf7fe66db4fdac7c7263f8b2523
https://pubmed.ncbi.nlm.nih.gov/30670702
https://pubmed.ncbi.nlm.nih.gov/30670702
Autor:
Yinqing Li, Arisa Kurabayashi, Winston X. Yan, Osamu Nureki, Ryuichiro Ishitani, Feng Zhang, F. Ann Ran, Hiroshi Nishimasu, Bernd Zetsche, Le Cong
Publikováno v:
PMC
Summary The RNA-guided DNA endonuclease Cas9 cleaves double-stranded DNA targets with a protospacer adjacent mot if (PAM) and complementarity to the guide RNA. Recently, we harnessed Staphylococcus aureus Cas9 (SaCas9), which is significantly smaller
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a46efb56eaed4f3fd2815acf664d37f5
https://doi.org/10.1016/j.cell.2015.08.007
https://doi.org/10.1016/j.cell.2015.08.007
Autor:
Phillip A. Sharp, Xuebing Wu, Jonathan S. Gootenberg, Winston X. Yan, Andrea J. Kriz, David A. Scott, Feng Zhang, Kira S. Makarova, Le Cong, Bernd Zetsche, Eugene V. Koonin, F. Ann Ran, Ophir Shalem
Publikováno v:
Nature
The RNA-guided endonuclease Cas9 has emerged as a versatile genome-editing platform. However, the size of the commonly used Cas9 from Streptococcus pyogenes (SpCas9) limits its utility for basic research and therapeutic applications that use the high
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9216b7a945011fcb40ebc763482e67c2
https://doi.org/10.1038/nature14299
https://doi.org/10.1038/nature14299
Publikováno v:
Nature Biotechnology. 33:139-142
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2eb4e16d472014f52c19234285701e3e
https://doi.org/10.1038/nbt.3149
https://doi.org/10.1038/nbt.3149
Autor:
Reza Mirzazadeh, Winston X. Yan, Silvano Garnerone, David Scott, Martin W. Schneider, Tomasz Kallas, Joaquin Custodio, Erik Wernersson, Yinqing Li, Linyi Gao, Yana Federova, Bernd Zetsche, Feng Zhang, Magda Bienko, Nicola Crosetto
Publikováno v:
Protocol Exchange.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::babc7ae59cb6dd619f3c3183926a96b5
https://doi.org/10.1038/protex.2017.018
https://doi.org/10.1038/protex.2017.018
Autor:
Omar O. Abudayyeh, David A. Scott, Jonathan Strecker, Feng Zhang, Bernd Zetsche, Jonathan S. Gootenberg
SummaryThe recently discovered class 2 CRISPR-Cas endonuclease Cpf1 offers several advantages over Cas9, including the ability to process its own array and requirement for just a single RNA guide. These attributes make Cpf1 promising for many genome
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::885d6729d5da32be31a3d340c9ebbfd0
https://doi.org/10.1101/134015
https://doi.org/10.1101/134015
Autor:
Omar O. Abudayyeh, Konstantin Severinov, Jonathan S. Gootenberg, Nerges Winblad, John van der Oost, Wen Y Wu, David A. Scott, Matthias Heidenreich, Jeroen Kneppers, Sourav R Choudhury, Iana Fedorova, Feng Zhang, Ellen M DeGennaro, Prarthana Mohanraju, Bernd Zetsche
Publikováno v:
Nature biotechnology
Nat. Biotechnol. 35, 31–34 (2017); published online 5 December 2016; corrected after print 12 January 2017 In the version of this article initially published, in Fig. 2j, the percentage for the targets Mecp2, Nlgn3, and Drd1 should be 15.2%, not 16
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10e69a6eda3e0da3bf8ed381f5a759a7
https://pubmed.ncbi.nlm.nih.gov/27918548
https://pubmed.ncbi.nlm.nih.gov/27918548