Zobrazeno 1 - 10
of 480
pro vyhledávání: '"Bernd, Nidetzky"'
Publikováno v:
Communications Chemistry, Vol 7, Iss 1, Pp 1-12 (2024)
Abstract Glycosylated derivatives of natural product polyphenols display a spectrum of biological activities, rendering them critical for both nutritional and pharmacological applications. Their enzymatic synthesis by glycosyltransferases is frequent
Externí odkaz:
https://doaj.org/article/8757e95a5aa04497b6a99324b924d712
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-10 (2024)
Abstract Understanding enzyme catalysis as connected to protein motions is a major challenge. Here, based on temperature kinetic studies combined with isotope effect measurements, we obtain energetic description of C-H activation in NAD-dependent UDP
Externí odkaz:
https://doaj.org/article/60207c4885d144d78f7ce81400ecb88f
Autor:
Johannes Bitter, Martin Pfeiffer, Annika J. E. Borg, Kirill Kuhlmann, Tea Pavkov-Keller, Pedro A. Sánchez-Murcia, Bernd Nidetzky
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-17 (2023)
Abstract Biological degradation of natural product glycosides involves, alongside hydrolysis, β-elimination for glycosidic bond cleavage. Here, we discover an O-glycoside β-eliminase (OGE) from Agrobacterium tumefaciens that converts the C3-oxidize
Externí odkaz:
https://doaj.org/article/716101903c9c4f90bd2ea47a7ac4134f
Publikováno v:
Microbial Cell Factories, Vol 22, Iss 1, Pp 1-15 (2023)
Abstract Background In whole-cell bio-catalysis, the biosystems engineering paradigm shifts from the global reconfiguration of cellular metabolism as in fermentation to a more focused, and more easily modularized, optimization of comparably short cas
Externí odkaz:
https://doaj.org/article/d72c0531657a48b3a431d6a016204d07
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-13 (2023)
Abstract As a crucial factor of their therapeutic efficacy, the currently marketed mRNA vaccines feature uniform substitution of uridine (U) by the corresponding C-nucleoside, pseudouridine (Ψ), in 1-N-methylated form. Synthetic supply of the mRNA b
Externí odkaz:
https://doaj.org/article/81978847c8294d6fb24accaa32dc37f7
Autor:
Katharina N. Schwaiger, Bernd Nidetzky
Publikováno v:
Microbial Cell Factories, Vol 21, Iss 1, Pp 1-16 (2022)
Abstract Background Continuous processing with enzyme reuse is a well-known engineering strategy to enhance the efficiency of biocatalytic transformations for chemical synthesis. In one-pot multistep reactions, continuous processing offers the additi
Externí odkaz:
https://doaj.org/article/fea2d08a30d4433b92260c1c594a9b54
Publikováno v:
Microbial Cell Factories, Vol 21, Iss 1, Pp 1-14 (2022)
Abstract Background Soluble cello-oligosaccharides (COS, β‐1,4‐D‐gluco‐oligosaccharides with degree of polymerization DP 2–6) have been receiving increased attention in different industrial sectors, from food and feed to cosmetics. Develop
Externí odkaz:
https://doaj.org/article/1645d6e9393e42a99449617814b7e671
Publikováno v:
BMC Biotechnology, Vol 21, Iss 1, Pp 1-13 (2021)
Abstract Background Published biocatalytic routes for accessing enantiopure 2-phenylpropanol using oxidoreductases afforded maximal product titers of only 80 mM. Enzyme deactivation was identified as the major limitation and was attributed to adduct
Externí odkaz:
https://doaj.org/article/7edc7aad74d54b7abec080f65829ed06
Publikováno v:
Biotechnology for Biofuels, Vol 14, Iss 1, Pp 1-15 (2021)
Abstract Background Cellodextrin phosphorylase (CdP; EC 2.4.1.49) catalyzes the iterative β-1,4-glycosylation of cellobiose using α-d-glucose 1-phosphate as the donor substrate. Cello-oligosaccharides (COS) with a degree of polymerization (DP) of u
Externí odkaz:
https://doaj.org/article/acac5fd0bdd446da8a24612858072cf5
Publikováno v:
Microbial Cell Factories, Vol 20, Iss 1, Pp 1-16 (2021)
Abstract Background Glucosylglycerol (2-O-α-d-glucosyl-sn-glycerol; GG) is a natural osmolyte from bacteria and plants. It has promising applications as cosmetic and food-and-feed ingredient. Due to its natural scarcity, GG must be prepared through
Externí odkaz:
https://doaj.org/article/37fd2515be984e9c8b66e8faaaad97e4