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pro vyhledávání: '"Bernard Mély-Goubert"'
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 601:315-327
Experiments have been designed to challenge the use of steady-state fluorescence polarization with 1,6-diphenyl-1,3,5-hexatriene as an evaluator of the fluidity of cell plasma membranes. We used paraffinic systems, of defined structure and compositio
Autor:
Murray H. Freedman, Barbara J. Trew-Marshall, Bernard Mély-Goubert, Nasim R. Khan, Claire G. Cupples
Publikováno v:
Cellular Immunology. 58:134-146
Normal quiescent T lymphocytes when activated with a nontoxic, mitogenic dose of A23187, a divalent cation ionophore, showed increased uptake of 45 Ca 2+ and [ 3 H]thymidine. When the T cells were labeled with a fluorescent apolar probe (1,6-diphenyl