Novel method for action potential measurements from intact cardiac monolayers with multiwell microelectrode array technology

Autor: Mike Clements, Jérome Chal, Denise D. Sullivan, Stacie A. Chvatal, Anthony M. Nicolini, James D. Ross, Bernard Fermini, Daniel C. Millard, Colin A. Arrowood, Hee Cheol Cho, David Wolfson, Heather Hayes

Publikováno v: Scientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)
Scientific Reports

The cardiac action potential (AP) is vital for understanding healthy and diseased cardiac biology and drug safety testing. However, techniques for high throughput cardiac AP measurements have been limited. Here, we introduce a novel technique for rel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::148042bae35424aec95d1a226ca06737
http://link.springer.com/article/10.1038/s41598-019-48174-5

Cross-site and cross-platform variability of automated patch clamp assessments of drug effects on human cardiac currents in recombinant cells

Autor: Georg Andrees Bohme, Jennifer B. Pierson, Sonja Stoelzle-Feix, Simon Hebeisen, Nina Brinkwirth, Carlos G. Vanoye, James Kramer, Gary Gintant, Jingsong Fan, Atsushi Ohtsuki, Dingzhou Li, Muthukrishnan Renganathan, Herbert M. Himmel, Bernard Fermini, Matthew Bridgland-Taylor, Edward Humphries, Khuram W. Chaudhary, John Imredy, Tim Strassmaier, Anders Lindqvist, Timm Danker, Liudmila Polonchuk

Publikováno v: Scientific Reports, Vol 10, Iss 1, Pp 1-15 (2020)
Scientific Reports

Automated patch clamp (APC) instruments enable efficient evaluation of electrophysiologic effects of drugs on human cardiac currents in heterologous expression systems. Differences in experimental protocols, instruments, and dissimilar site procedure

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::793dcd388f38431a41186ab3f240d140
https://doi.org/10.1038/s41598-020-62344-w

Cardiac sodium channel antagonism – Translation of preclinical in vitro assays to clinical QRS prolongation

Autor: Donglin Guo, Bernard Fermini, David S. Ramirez, Stephen Jenkinson, Asser Bassyouni, Jason Cordes, Todd Wisialowski, Sunny Z. Sun, David M. Potter, Jill Steidl-Nichols

Publikováno v: Journal of Pharmacological and Toxicological Methods. 89:9-18

Introduction Cardiac sodium channel antagonists have historically been used to treat cardiac arrhythmias by preventing the reentry of the electrical impulse that could occur following myocardial damage. However, clinical studies have highlighted a si

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8af8646dabdbc80e028ae938b088d883
https://doi.org/10.1016/j.vascn.2017.10.002

Use of automated patch clamp in cardiac safety assessment: Past, present & future perspectives

Autor: Damian C, Bell, Bernard, Fermini

Publikováno v: Journal of Pharmacological and Toxicological Methods. 111:107114

There is no doubt that automated patch clamp (APC) technology has revolutionized research in biomedical science. High throughput ion channel screening is now an integral part of the development and safety profiling of the majority of new chemical ent

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c9de29a0a9bb485b76845a803311804
https://doi.org/10.1016/j.vascn.2021.107114

Cardiac voltage-gated ion channels in safety pharmacology: Review of the landscape leading to the CiPA initiative

Autor: Simon Authier, Michael J. Curtis, John Koerner, Bernard Fermini, Michael K. Pugsley, Hai Huang, Michael V. Accardi

Publikováno v: Huang, H, Pugsley, M K, Fermini, B, Curtis, M J, Koerner, J, Accardi, M & Authier, S 2017, ' Cardiac voltage-gated ion channels in safety pharmacology: Review of the landscape leading to the CiPA initiative ', Journal of Pharmacological and Toxicological Methods . https://doi.org/10.1016/j.vascn.2017.04.002

Voltage gated ion channels are central in defining the fundamental properties of the ventricular cardiac action potential (AP), and are also involved in the development of drug-induced arrhythmias. Many drugs can inhibit cardiac ion currents, includi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::492a5ca754b0c7eb06d795dc6c4065fa
https://doi.org/10.1016/j.vascn.2017.04.002