Extending chemical perturbations of the ubiquitin fitness landscape in a classroom setting reveals new constraints on sequence tolerance.

Autor: Mavor D; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Barlow KA; Bioinformatics Graduate Group, University of California, San Francisco 94158, USA., Asarnow D; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Birman Y; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Britain D; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Chen W; Bioinformatics Graduate Group, University of California, San Francisco 94158, USA., Green EM; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Kenner LR; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Mensa B; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Morinishi LS; Bioinformatics Graduate Group, University of California, San Francisco 94158, USA., Nelson CA; Bioinformatics Graduate Group, University of California, San Francisco 94158, USA., Poss EM; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Suresh P; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Tian R; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Arhar T; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Ary BE; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Bauer DP; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Bergman ID; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Brunetti RM; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Chio CM; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Dai SA; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Dickinson MS; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Elledge SK; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Helsell CVM; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Hendel NL; Bioinformatics Graduate Group, University of California, San Francisco 94158, USA., Kang E; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Kern N; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Khoroshkin MS; Bioinformatics Graduate Group, University of California, San Francisco 94158, USA., Kirkemo LL; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Lewis GR; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Lou K; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Marin WM; Bioinformatics Graduate Group, University of California, San Francisco 94158, USA., Maxwell AM; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., McTigue PF; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Myers-Turnbull D; Bioinformatics Graduate Group, University of California, San Francisco 94158, USA., Nagy TL; Bioinformatics Graduate Group, University of California, San Francisco 94158, USA., Natale AM; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Oltion K; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Pourmal S; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Reder GK; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Rettko NJ; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Rohweder PJ; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Schwarz DMC; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Tan SK; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Thomas PV; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Tibble RW; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Town JP; Bioinformatics Graduate Group, University of California, San Francisco 94158, USA., Tsai MK; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Ugur FS; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Wassarman DR; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Wolff AM; Biophysics Graduate Group, University of California, San Francisco 94158, USA., Wu TS; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco 94158, USA., Bogdanoff D; Department of Biochemistry and Biophysics, University of California, San Francisco 94158, USA., Li J; Department of Chemistry Undergraduate Program, University of California, Davis 95616, USA., Thorn KS; Department of Biochemistry and Biophysics, University of California, San Francisco 94158, USA., O'Conchúir S; Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biology (QBI), San Francisco 94158, USA., Swaney DL; Department of Cellular and Molecular Pharmacology, California Institute for Quantitative Biology (QBI), San Francisco 94158, USA., Chow ED; Department of Biochemistry and Biophysics, University of California, San Francisco 94158, USA., Madhani HD; Department of Biochemistry and Biophysics, University of California, San Francisco 94158, USA., Redding S; Department of Biochemistry and Biophysics, University of California, San Francisco 94158, USA., Bolon DN; Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester 01655, USA., Kortemme T; Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biology (QBI), San Francisco 94158, USA., DeRisi JL; Department of Biochemistry and Biophysics, University of California, San Francisco 94158, USA., Kampmann M; Department of Biochemistry and Biophysics, University of California, San Francisco 94158, USA martin.kampmann@ucsf.edu jfraser@fraserlab.com.; Institute for Neurodegenerative Diseases, University of California, San Francisco 94158, USA., Fraser JS; Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biology (QBI), San Francisco 94158, USA martin.kampmann@ucsf.edu jfraser@fraserlab.com.

Publikováno v: Biology open [Biol Open] 2018 Jul 23; Vol. 7 (7). Date of Electronic Publication: 2018 Jul 23.